Proteomic analysis of seed viability in maize

• Autorzy: Wu X. , Liu H. , Wang W. , Chen S. , Hu X. , Li C.
• Języki publikacji: EN

Abstrakty

EN

To identify specific proteins related to maize seed viability, seeds of Zhengdan 958 (one of the highyield maize hybrids in China) were sorted based on viability evaluation with triphenyltetrazolium chloride (TTC) assay and used for comparative proteomic analysis. After TTC staining, embryos of high-viability seeds were deep red (R type), while embryos of dead seeds were white (W type). Proteomic analysis revealed that 28 protein spots identified were differently expressed significantly between R and W embryos, of which 20 were up-regulated and 8 down-regulated in R embryos. Among them were proteins involved in stress response, protein folding, and stabilization, as wells as proteins related to nutrient reservoir and metabolism. Prominently, small heat shock proteins, late embryogenesis abundant (LEA) proteins, and antioxidant enzymes were highly up-regulated, while two proteases were highly down-regulated in R embryos compared to W embryos. One of LEA proteins was EMB564, which declined in abundance during artificial aging of seeds. Our results suggested an association of EMB564 with maize seed viability. It would be of interest to use these small proteins to develop quick tests for seed quality.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 1
• Opis fizyczny: p.181-191,fig.,ref.

Twórcy

autor

Wu X.

• College of Life Science, Henan Agricultural University, Zhengzhou, China
• Huanghuaihai Regional Innovation Center for Maize Technology, Ministry of Agriculture, Zhengzhou, China

autor

Liu H.

• College of Life Science, Henan Agricultural University, Zhengzhou, China

autor

Wang W.

• College of Life Science, Henan Agricultural University, Zhengzhou, China
• Huanghuaihai Regional Innovation Center for Maize Technology, Ministry of Agriculture, Zhengzhou, China

autor

Chen S.

• College of Life Science, Henan Agricultural University, Zhengzhou, China

autor

Hu X.

• College of Life Science, Henan Agricultural University, Zhengzhou, China

autor

Li C.

• Huanghuaihai Regional Innovation Center for Maize Technology, Ministry of Agriculture, Zhengzhou, China

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Identyfikatory

DOI

10.1007/s11738-010-0536-4