Interaction between indocyanine green and gadobutrol in cerebellar granule neurons cultures

• Autorzy: Zieminska E. , Toczylowska B. , Goch G. , Liebert S.
• Języki publikacji: EN

Abstrakty

EN

In experiments we used indocyanine green (ICG), and gadobutrol (Gad) contrast dyes. There is no information about parallel application of ICG and Gad, therefore we decided to study their toxicity using primary cerebellar granule cells culture (CGC). The aim of study was to assess the minimal ICG concentration which evokes neurotoxicity. 30 min exposition to 75 and 125 μM ICG resulted in neurotoxicity. We observed imbalance in calcium homeostasis (extra- and intracellular) after addition of ICG, which can be one of the mechanisms of ICG neurotoxicity. We measured absorbance and NMR spectra for 25–125 µM ICG concentrations in three solvents. Gad contrast media mixed with ICG were also measured and neurotoxicity of this mixture was examined. The shape of absorption and NMR spectra show differences between water, water with 2.3 mM Ca2+ and Locke25 for all analyzed ICG concentrations. Other possible mechanism of ICG neurotoxicity can be dose dependent oligomerization of ICG. We did not observe any toxic effect of Gad on CGC. Protective effect on surviving neurons after treatment of ICG, dependent on Gad dose and sequence of its administration (before > simultaneously > after addition of ICG) was observed. However, the mechanism of this phenomenon remains not clear. Supported by grant 2011/03/B/ST7/02576.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: Suppl.1
• Opis fizyczny: p.29

Twórcy

autor

Zieminska E.

• MossakowskiMedical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Toczylowska B.

• Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland
• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

autor

Goch G.

• Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

autor

Liebert S.

• Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Warsaw, Poland