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2012 | 56 | 4 |
Tytuł artykułu

Genotypic markers of Yersinia enterocolitica O:9 isolated from cows positive in serological examination for bovine brucellosis

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of the study was to perform a molecular investigations for the presence of pathogenicity genotypie markers of Y. enterocolitica 0:9 isolated from cattle, in which initially positive serological reactions for brucellosis were observed. Almost all isolates were αil-, ystA- and myfA-positive (n=19). On the other hand, one isolate, which harboured plasmid encoding gene yadA was αil-, ystA- and myfA-negative. The plasmid encoding yadA marker was present in half of the isolates tested. None of the examined isolates was ystß-positive. The results of the investigations revealed that the Y. enterocolitica O:9 isolates, related to false positive serological results for brucellosis, may be also potentially pathogenic for humans, due to the presence of chromosomal and plasmid- encoded molecular markers.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
56
Numer
4
Opis fizyczny
p.479-482,ref.
Twórcy
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, 24-100 Pulawy, Poland
Bibliografia
  • 1. Garin-Bastuji B., Hummel N., Gerbier G., Cau C., Pouillot R., Da Costa M., Fontaine J.J.: Nonspecific serological reactions in the diagnosis of bovine brucellosis: experimental oral infection of cattle with repeated doses of Yersinia enterocolitica O:9. Vet Microbiol 1999, 66, 223-233.
  • 2. Gerbier G., Garin-Bastuji B., Pouillot R., Very P., Cau C., Berr V., Dufour B., Moutou F.: False positive serological reactions in bovine brucellosis: evidence of the role of Yersinia enterocolitica serotype O:9 in a field trial. Vet Res 1997, 28, 375-383.
  • 3. Grattarola C., Gennero M.S., Zoppi S., Bergagna S., Chiavacci L., Dondo A.: Yersinia enterocolitica and its role in bovine brucellosis monitoring : a statistical analysis. Epidémiol Santé Anim 2006, 50, 153-155.
  • 4. Harnett N., Lin Y.P., Krishahn C.: Detection of pathogenic Yersinia enterocolitica using the multiplex polymerase chain reaction. Epidemiol Infect 1996, 117, 59-67.
  • 5. Hilbink F., Fenwick S.G., Thompson E.J., Kittelberger R., Penrose M., Ross G.P.: Non-specific seroreactions against Brucella abortus in ruminants in New Zealand and the presence of Yersinia enterocolitica O:9. N Z Vet J 1995,43, 175-178.
  • 6. Instruction No. 401/BB-16/2010 of the Chief Veterinary Officer, Warsaw, 2010.
  • 7. Iriarte M., Cornelis G.R.: MyfF, an element of the network regulating the synthesis of fibriallae in Yersinia enterocolitica. J Bacteriol 1995, 177, 738-744.
  • 8. Kapperund G., Vardund T., Skjerve E., Hornes E., Michaelsen T.E.: Detection of pathogenic Yersinia enterocolitica in food and water by immunomagnetic separation, nested polymerase chain reactions and colorimetric detection of amplified DNA. Appl Environ Microbiol 1993, 59, 2938-2944.
  • 9. Kot B., Piechota M., Jakubczak A.: Analysis of occurrence of virulence genes among Yersinia enterocolitica isolates belonging to different biotypes and serotypes. Pol J Vet Sci 2010, 13, 13-19.
  • 10. Kot B.: Chromosomally and plasmid-encoded virulence determinants of Y. enterocolitica. Medycyna Wet 2010, 66, 294-298.
  • 11. Lubeck P.S., Skurnik M., Akrens., Hoorfar J.: A Multiplex PCR-detection assay for Yersinia enterocolitica serotype O:9 and Brucella spp. based on perosamine syntethase gene. Adv Exp Med Biol 2003, 529, 451-455.
  • 12. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, OIE, Paris, 2010 http://www.oie.int/international-standard- setting/terrestrial-manual/access-online/
  • 13. Nielsen K., Smith P., Widdison J., Gall D., Kelly L., Kelly W., Nicoletti P.: Serological relationship between cattle exposed to Brucella abortus, Yersinia enterocolitica O:9 and Escherichia coli O:157:H7. Vet Microbiol 2004, 100, 25-30.
  • 14. Platt-Samoraj A., Ugorski M., Szweda W., Szczerba- Turek A., Wojciech K., Procajło Z.: Analysis of the presence of αil, ystA and ystB genes in Yersinia enterocolitica strains isolated from aborting sows and aborted fetuses. J Vet Med B 2006, 53, 341-346.
  • 15. Riber U., Jungersen G.: Cell-mediated immune responses differentiate infections with Brucella suis from Yersinia enterocolitica serotype O:9 in pigs. Vet Immunol Immunopathol 2007, 116, 13-25.
  • 16. Scientific Opinion of the Panel on Biological Hazard (BIOHAZ) on monitoring and identification of human enteropathogenic Yersinia spp. EFSA J 2007, 595, 1-30.
  • 17. Scientific Report of EFSA and ECDC. The European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks in 2010. EFSA J 2012, 3, 2597.
  • 18. Szulowski K., Iwaniak W., Weiner M., Złotnicka J., Szymajda M., Zaręba Z., Czępińska H.: Diagnosis and epidemiological condition of bovine brucellosis in Poland. Medycyna Wet 2012, 68, 110-113.
  • 19. Thoerner P., Bin Kingombe C.I., Bogli-Stuber K., Bissig- Choisant B., Wassenaar T.M., Frey J., Jemmi T.: PCR detection for virulence genes in Yersinia enterocolitica and Yersinia pseudotuberculosis and investigation of virelence gene distribution. Appl Environ Microbiol 2003, 69, 1810-1816.
  • 20. Wannet W.J., Reessink M., Brunings H.A., Maas H.M.: Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR. J Clin Microbiol 2001, 39, 4483-4486.
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  • 22. Weiner M., Złotnicka J., Iwaniak W., Szulowski K.: Development of a multiplex PCR for identification of Brucella sp. and cross-reacting Yersinia enterocolitica O:9. Bull Vet Inst Pulawy 2011, 55, 603-607.
  • 23. Weynants V., Tibor A., Denoel P.A., Saegerman C., Godfroid J., Thiange P., Letesson J.J.: Infection of cattle with Yersinia enterocolitica O:9 a cause of the false positive serological reactions in bovine brucellosis diagnostic tests. Vet Microbiol 1996, 48, 101-112.
Typ dokumentu
Bibliografia
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