A new anti-counterfeiting method: fluorescent labeling by safranine T in tobacco seed

• Autorzy: Guan Y. , Hu J. , Li Y. , Ma W. , Zheng Y.
• Języki publikacji: EN

Abstrakty

EN

Counterfeit seeds cause a large loss in seed industry and crop production. Present study was undertaken to find a new anti-counterfeiting technology by labeling seeds with a fluorescent compound, safranine T (ST). Response surface analysis (RSA) experiment with two factors (ST concentration and seed soaking time) of 14 combinations was performed to investigate the ST effects on seed vigor and fluorescence showing in seedlings of two tobacco varieties, MS Yunyan 85 and Honghua Dajinyuan. After soaking in ST solutions, tobacco seeds were germinated for 16 days, then the germination index (GI), vigor index (VI), length of roots and shoots were determined. The optimum combinations of ST concentration and soaking time on GI, VI and length of shoots and roots were 0.57–0.69 mg/ml and 6.77–9.34 h for MS Yunyan 85 and 0.51–0.80 mg/ml and 5.81–7.39 h for Honghua Dajinyuan, respectively. Meanwhile, tobacco seedlings, treated with ST solutions from 0.3 to 1.5 mg/ml showed obviously fluorescence under illumination of green light. It suggests that soaking tobacco seeds in safranine T solution of suitable concentration can be used as a labeling technique for anti-counterfeiting in tobacco seeds.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 4
• Opis fizyczny: p.1271-1276,fig.,ref.

Twórcy

autor

Guan Y.

• Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China

autor

Hu J.

• Seed Science Center, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China

autor

Li Y.

• China Tobacco Breeding Research Southern Center, Yunnan Tobacco Science Research Institute, Yuxi, 653100, China

autor

Ma W.

• China Tobacco Breeding Research Southern Center, Yunnan Tobacco Science Research Institute, Yuxi, 653100, China

autor

Zheng Y.

• China Tobacco Breeding Research Southern Center, Yunnan Tobacco Science Research Institute, Yuxi, 653100, China

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