Effect of bioreactor angle and aeration rate on growth and hydromechanics parameters in bioreactor culture of ginseng suspension cells

• Autorzy: Dong Y , Gao W.-Y. , Man S. , Zuo B. , Wang J. , Huang L. , Xiao P.
• Języki publikacji: EN

Abstrakty

EN

Suspension cells of Panax ginseng C.A. Meyer were cultivated in 3-L balloon-type bubble bioreactors and the bioreactor with the angle of 90º at the bottom side was optimized. The gaseous composition in plant cell and tissue cultures is regarded as an important factor affecting the plant growth. Gas hold-up was remarkably higher in the bioreactor with an angle of 90º than the other ones. Aeration rates impacted on the growth ratio, the specific O2 uptake rate (SOUR) of ginseng cells were investigated. 0.4 vvm was selected as the optimal aeration rate with a dry weight of 6.45 g L-1. The specific O2 uptake rate in the culture time was detected and reached the top value at the maximum growth ratio.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 05
• Opis fizyczny: p.1497-1501,fig.,ref.

Twórcy

autor

Dong Y

• Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China

autor

Gao W.-Y.

• School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin 300072, China

autor

Man S.

• Key Laboratory of Industrial Fermentation Microbiology, Tianjin University of Science and Technology, Ministry of Education, Tianjin 300457, China

autor

Zuo B.

• Key Laboratory of Industrial Fermentation Microbiology, Tianjin University of Science and Technology, Ministry of Education, Tianjin 300457, China

autor

Wang J.

• School of Pharmaceutical Science and Technology, Tianjin University, No. 92 Weijin Road, Nankai District, Tianjin 300072, China

autor

Huang L.

• Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China

autor

Xiao P.

• Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100193, China

Bibliografia

• Ali MB, Yu KW, Hahn EJ, Paek KY (2005) Differential responses of anti-oxidants enzymes, lipoxygenase activity, ascorbate content and the production of saponins in tissue cultured root of mountain Panax ginseng C.A. Mayer and Panax quinquefolium L. in bioreactor subjected to methyl jasmonate stress. Plant Sci 169:83–92
• Angelova N, Kong HW, van der Heijden R, Yang SY, Choi YH, Kim HK, Wang M, Hankemeier T, van der Greef J, Xu G, Verpoorte R (2008) Recent methodology in the phytochemical analysis of ginseng. Phytochem Anal 19:2–16
• Azechi S, Hashimoto T, Murata A, Miyakita I, Ishii H (1985) Dynamic measurement of volumetric oxygen transfer coefficient. Jpn Tobacco Cult Pulb Crop 20:73–85
• Ballica R, Ryu DD (1993) Effects of rheological properties and mass transfer on plant cell bioreactor performance: production of tropane alkaloids. Biotechnol Bioeng 42:1181–1189
• Cascaval D, Galaction A, Oniscu C (2003) Studies on oxygen mass transfer in stirred bioreactors. Chem Ind 57:276–287
• Chinese Pharmacopoeia, 2010 (edition), p 8 Han J, Zhong JJ (2003) Effects of oxygen partial pressure on cell growth and ginsenoside and polysaccharide production in high density cell cultures of P. Notoginseng. Enzym Microb Technol 32:498–503
• Huang T, Gao WY, Wang J, Cao Y, Zhao YX, Huang LQ, Liu CX (2010) Selection and optimization of a high-production tissue culture of Panax ginseng C.A. Meyer. Acta Physiol Plant 32:765–772
• Jeong GT, Park DH (2005) Comparative evaluation of modified bioreactors for enhancement of growth and secondary metabolite biosynthesis using Panax ginseng hairy roots. Biotechnol Bioprocess Eng 10:528–534
• Jeong CS, Chakrabarty D, Hahn EJ, Lee HL, Paek KY (2006) Effects of oxygen, carbon dioxide and ethylene on growth and bioactive compound production in bioreactor culture of ginseng adventitious roots. Biochem Eng J 27:252–263
• Jurascık M, Blažej M, Annus J, Markos J (2006) Experimental measurements of volumetric mass transfer coefficient by the dynamic pressure-step method in internal loop airlift reactors of different scale. Chem Eng J 125:81–87
• Kieran PM, MacLoughlin PF, Malone DM (1997) Plant cell suspension cultures: some engineering considerations. J Biotechnol 59:39–52
• Leckie F, Scragg AH, Cliffe KC (1991) An investigation into the role of initial KLA on the growth and alkaloid accumulation by cultures of Catharanthus roseus. Biotechnol Bioeng 37:364–370
• Naval MV, Go´mez-Serranillos MP, Carretero ME, Villar AM (2007) Neuroprotective effect of a ginseng (Panax ginseng) root extract on astrocytes primary culture. J Ethnopharmacol 112:262–270
• Paek KY, Hahn EJ, Son SH (2001) Application of bioreactors for large-scale micropropagation systems of plants. In Vitro Cell Dev Biol Plant 37:149–157
• Paek KY, Murthy HN, Hahn EJ, Zhong JJ (2009) Large scale culture of ginseng adventitious roots for production of ginsenosides. Adv Biochem Eng Biotechnol 113:151–176
• Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153
• Shin KS, Murthy HN, Ko JY, Paek KY (2002) Growth and betacyanin production by hairy roots of Beta vulgaris in airlift bioreactors. Biotechnol Lett 24:2067–2069
• Su W, Sun AJ, Xu DL, Zhang HQ, Yang L, Yuan LY, Jia JG, Zou YZ, Wu YL, Wang KQ, Ge JB (2010) Inhibiting effects of total saponins of Panax ginseng on immune maturation of dendritic cells induced by oxidized-low density lipoprotein. Cell Immunol 263:99–104
• Sun JH, Hu SH, Song XM (2007) Adjuvant effects of protopanaxadiol and protopanaxatriol saponins from ginseng roots on the immune responses to ovalbumin in mice. Vaccine 25:1114–1120
• Tang W, Eisenbrand G (1992) Panax ginseng C A. Meyer Chinese drugs of plant origin. Springer, Berlin, pp 710–737
• Thimmaraju R, Bhagyalakshmi N, Narayan MS, Ravishankar GA (2003) Kinetics of pigment release from hairy root cultures of Beta vulgaris under the influence of pH, sonication, temperature and oxygen stress. Process Biochem 38:1069–1076
• Wang B, Wang W, Han H, Hu H, Zhuang H (2012) Nitrogen removal and simultaneous nitrification and denitrification in a fluidized bed step-feed process. J Environ Sci 24:303–308
• Wu J, Zhong JJ (1999) Production of ginseng and its bioactive components in plant cell culture: current technological and applied aspects. J Biotechnol 68:89–99
• Zhou H, Hou SZ, Luo P, Zeng B, Wang JR, Wong YF, Jiang ZH, Liu L (2011) Ginseng protects rodent hearts from acute myocardial ischemia–reperfusion injury through GR/ER-activated RISK pathway in an endothelial NOS-dependent mechanism. J Ethnopharmacol 135(2):287–298

Uwagi

EN

rekord w opracowaniu