Improvement of an RNA purification method for grapevine (Vitis vinifera L.) suitable for cDNA library construction

• Autorzy: Daldoul S. , Chenenanoui S. , Mliki A. , Hofer M.
• Języki publikacji: EN

Abstrakty

EN

A method is described, which consistently yields high quality total RNA from grapevine. Dissolving of crude RNA pellets in borate-containing buffer, instead of normally used water before a selective lithium chloride precipitation, was found to be a critical step, leading to a 2.5-fold increase of yield. The resulting RNA preparations were suitable for standard downstream applications and also for cDNA library construction. The method worked efficiently and reproducibly and could easily be scaled from milligram to gram quantities of plant material grown in hydroponic culture, sandy soil or Perlite. It was applied to different kinds of grapevine tissues (leaves, stem) and, after additional adaptation of the protocol, to roots.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 4
• Opis fizyczny: p.871-875,fig.,ref.

Twórcy

autor

Daldoul S.

• Laboratorie de Physiologie Moleculaire de la Vigne, B.P.901, 2050 Hammam-Lif, Tunisia
• RLP AgroScience GmbH, AlPlanta, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany

autor

Chenenanoui S.

• Laboratorie de Physiologie Moleculaire de la Vigne, B.P.901, 2050 Hammam-Lif, Tunisia

autor

Mliki A.

• Laboratorie de Physiologie Moleculaire de la Vigne, B.P.901, 2050 Hammam-Lif, Tunisia

autor

Hofer M.

• RLP AgroScience GmbH, AlPlanta, Breitenweg 71, 67435 Neustadt an der Weinstrasse, Germany

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