Metoda cytometrii przepływowej w ocenie zdrowotności gruczołu mlekowego przeżuwaczy

• Autorzy: Lisiecka U. , Kostro K. , Wojtaszczyk P. , Zmuda A.

Warianty tytułu

EN

Flow cytometry method in the evaluation of the health status of mammary glands in ruminants

• Języki publikacji: PL

Abstrakty

EN

One of the practical applications of flow cytometry is the possibility of the analysis of leukocyte populations which participate in immunological phenomena in ruminant mammary glands. By use of the flow cytometry method the investigations can be conducted in a quick, precise and highly repeatable manner. Not only the quantitative assessment of immune cells present in milk is possible, but also the assessment of their qualitative variety and particular subpopulation affiliations. The differences in specific subpopulations content which are present in mammary glands in distinct physiological conditions (lactation and dry period) enable a quick and precise evaluation of immunological status and the recognition of mechanisms which take part in the immune resistance of the gland. With flow cytometry analysis of leukocyte surface antigens, apoptosis and phagocytosis, it is possible to understand the function of immune cells and immunological phenomena in mammary glands in conditions of health and disease. Flow cytometry could be successfully applied for monitoring the intensity of inflammation processes during the course of mastitis and causal and symptomatic therapy effectiveness assessment. Thus it creates new possibilities for developing more efficient treatment and prevention strategies for mastitis, which is of great significance in the dairy industry because of the possibility of preventing economic losses and increasing the safety of dairy products of animal origin.

Słowa kluczowe

PL

przezuwacze; krowy; kozy; owce; gruczoly mlekowe; okres laktacji; okres zasuszenia; mleko; wydzielina gruczolu mlekowego; leukocyty; aktywnosc bojcza; aktywnosc fagocytarna; cytometria przeplywowa; zdrowotnosc wymion; zapalenie gruczolu mlekowego; diagnostyka wczesna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2012
• Tom: 68
• Numer: 08
• Opis fizyczny: s.461-467,bibliogr.

Twórcy

autor

Lisiecka U.

• Katedra Epizootiologii i Klinika Chorób Zakaźnych, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Głęboka 30, 20-612 Lublin

autor

Kostro K.

autor

Wojtaszczyk P.

autor

Zmuda A.

Bibliografia

• 1.Asai K., Kai K., Rikiishi H., Sugawara S., Maruyama Y., Yamaguchi T., Ohta M., Kumagai K.: Variation in CD4+T and CD8+T lymphocyte subpopulations in bovine mammary gland secretions during lactating and non-lactating periods. Vet. Immunol. Immunopathol. 1998, 65, 51-61.
• 2.Asai K., Komine Y., Kozutsumi T., Yamaguchi T., Komine K., Kumagai K.: Predominant subpopulations of T lymphocytes in the mammary gland secretions during lactation and intraepithelial T lymphocytes in the intestine of dairy cows. Vet. Immunol. Immunopathol. 2000, 73, 233-240.
• 3.Bergonier D., DeCremoux R., Rupp R., Lagriffoul G., Berthelot X.: Mastitis of dairy small ruminants. Vet. Res. 2003, 34, 689-716.
• 4.Bischof R. J., Meeusen E. N. T.: Cellular kinetics of an allergic-type response in a sheep mammary gland model of inflammation. Clin. Exp. Allergy 2002, 32, 619-626.
• 5.Boutet P., Boulanger D., Gillet L., Vanderplasschen A., Closset R., Bureau F., Lekeux P.: Delayed neutrophil apoptosis in bovine subclinical mastitis. J. Dairy Sci. 2004, 87, 4104-4114.
• 6.Denis M., Parlane N. A., Lacy-Hulbert S. J., Summers E. L., Buddle B. M., Wedlock D. N.: Bactericidal activity of macrophages against Streptococcus uberis is different in mammary gland secretions of lactating and drying off cows. Vet. Immunol. Immunopathol. 2006, 114, 111-120.
• 7.Guiguen F., Greenland T., Pardo E., Panaye G., Mornex J. F.: Flow cytometric analysis of goat milk lymphocytes: subpopulations and adhesion molecule expression. Vet. Immunol. Immunopathol. 1996, 53, 173-178.
• 8.Koess C., Hamann J.: Detection of mastitis in the bovine mammary gland by flow cytometry at early stages. J. Dairy Res. 2008, 75, 225-232.
• 9.Leitner G., Eligulashvily R., Krifucks O., Perl S., Saran A.: Immune cell differentiation in mammary gland tissues and milk of cows chronically infected with Staphylococcus aureus. J. Vet. Med. 2003, 50, 45-52.
• 10.Owens M. A., Loken M. R.: Flow Cytometry Principles for Clinical Laboratory Practice. Quality assurance for quantitative immunophenotyping. Wiley-Liss, Inc., New York 1995.
• 11.Paape M. J., Bannerman D. D., Zhao X., Lee J. W.: The bovine neutrophil: structure and function in blood and milk. Vet. Res. 2003, 34, 597-627.
• 12.Park Y. H., Fox L. K., Hamilton M. J., Davis W. C.: Bovine mononuclear leukocyte subpopulations in peripheral blood and mammary gland secretions during lactation. J. Dairy Sci. 1992, 75, 998-1006.
• 13.Park Y. H., Joo Y. S., Park J. Y., Moon J. S., Kim S. H., Kwon N. H., Ahn J. S., Davis W. C., Davies Ch. J.: Characerization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows. J. Vet. Sci. 2004, 5, 29-39.
• 14.Peerson-Waller K., Colditz I. G.: Expression of surface antigens on blood and mammary leukocytes in lactating and dry ewes. Vet. Immunol. Immunopathol. 1998, 62, 273-278.
• 15.Redelmann D., Butler S., Robison J., Garner D.: Identification of inflammatory cells in bovine milk by flow cytometry. Cytometry 1988, 9, 4632-468.
• 16.Riollet P., Rainard P., Poutrel B.: Cell subpopulations and cytokine expression in cow milk in response to chronic Staphylococcus aureus mastitis. J. Dairy Sci. 2001, 84, 1077-1084.
• 17.Rivas A. L., Quimby F. W., Coksaygan O., Olmstead L., Lein D. H.: Longitudinal evaluation of CD4+ and CD8+ peripheral blood and mammary gland lymphocytes in cows experimentally inoculated with Staphylococcus aureus. Can. J. Vet. Res. 2000, 64, 232-237.
• 18.Rivas A. L., Tadevosyan R., Gorewit R. C., Anderson K. L., Lyman R., Gonzalez R. N.: Relationships between the phagocytic ability of milk macrophages and polymorphonuclear cells and somatic cell counts in uninfected cows. Can. J. Vet. Res. 2006, 70, 68-74.
• 19.Sladek Z., Rysanek D., Ryznarova H., Faldyna M.: The role of neutrophil apoptosis during experimentally induced Streptococcus uberis mastitis. Veterinarni Medicina Praha 2006, 9, 437-447.
• 20.Sordillo L. M., Shafer-Weaver K., DeRosa D.: Immunobiology of the mammary gland. J. Dairy Sci. 1997, 80, 1851-1865.
• 21.Smith E., Burvenich Ch., Guitry A. J., Heyneman R., Massart-Leen A.: Diapedesis across mammary epithelium reduces phagocytic and oxidative burst of bovine neutrophils. Vet. Immunol. Immunopathol. 1999, 68, 169-176.
• 22.Tian S. Z., Chang Ch. J., Chiang Ch. Ch., Peh H. Ch., Huang M. Ch., Lee J. W., Zhao X.: Comparison of morphology, viability and function between blood and milk neutrophils from peak lactating goats. Can. J. Vet. Res. 2005, 69, 39-45.
• 23.Winnicka A., Kluciński W., Hoser G., Sikora J., Kawiak J.: Flow cytometry analysis of milk and peripheral blood cells from goats during lactation. J. Vet. Med. 1999, 46, 459-464.
• 24.Van Oostveldt K., Vangroenweghe F., Dosogne H., Burvenich Ch.: Apoptosis and necrosis of blood and milk polymorphonuclear leukocytes in early and midlactating healthy cows. Vet. Res. 2001, 32, 617-622.
• 25.Vermes I., Haanen C., Steffens-Nakken H., Reutelingsperger C.: A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled annexin V. J. Immunol. Methods 1995, 184, 39-51.
• 26.Zeman K.: Wykorzystanie cytometrii przepływowej do badań granulocytów obojętnochłonnych. Mat. I Konf. Szkolen. "Przeciwciała monoklonalne w immunologii i diagnostyce". Puławy, 5-7 czerwca 1995, s. 99-117.