Wykorzystanie techniki FISH w badaniach przebiegu mejozy u buhajow

• Autorzy: Rejduch B , Kozubska-Sobocinska A.
• Języki publikacji: PL

Abstrakty

EN

The aim of this work was to assess the meiosis process in bulls on the basis of observations of the X-Y bivalent. The assessment was made using FISH technique in successive stages of the meiotic division. Gonad specimens were taken postmortem from three Red-and-White Lowland bulls that had originated from the Experimental Station Ltd of the National Research Institute of Animal Production in Grodziec Śląski. The animals were chosen on account of their semen parameters, which were lower than herd average. Meiotic chromosome preparations were made as described by Evans in 1964. Hybridization was carried out using molecular probes that identify bull X and Y chromosomes. A total of 38 spermatogonia and 144 primary spermatocytes at different stages of the meiotic division were tested. The analysis of hybridization signals (red-purple indicating the Y chromosome and yellow indicating the X chromosome) in bull gonad cells did not show any abnormalities in the meiosis process. A 90% dissociated X-Y bivalent was only observed in five spermatocytes at metaphase I. It is therefore concluded that these minor changes did not lower the semen parameters of the investigated animals.

Słowa kluczowe

PL

technika FISH; bydlo; chromosomy mejotyczne; mejoza; buhaje; cytogenetyka

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2006
• Tom: 62
• Numer: 01
• Strony: 96-98
• Opis fizyczny: s.96-98,fot.,tab.,bibliogr.

Twórcy

autor

Rejduch B

• Instytut Zootechniki, ul. Krakowska 1, 32-083 Balice

autor

Kozubska-Sobocinska A.

Bibliografia

• 1. Beechey C. V.: X-Y chromosome dissociation and sterility in the mouse. Cytogenet. Cell Genet. 1973, 12, 60-67.
• 2. Evans E. P., Breckon G., Ford C. E.: An air drying method of meiotic preparation from mammalian testes. Cytogenetics 1964, 3, 289-295.
• 3. Guan X. Y., Meltzer P. S., Cao J., Trent J. M.: Rapid generation of region specific clones by chromosome microdissection: Isolation of DNA from a region frequently detect in malignant melanoma. Genomic 1992, 14, 680-684.
• 4. Hassanane M., Kovacs A., Laurent P., Linblad K., Gustavsson I.: Simultaneous detection of X- and Y- bearing bull spermatozoa by double colour fluorescence in situ hybridization. Hun. J. Anim. Prod. 1999, 48, s. 128.
• 5. Laurent P., Hayes H., Petit E., Levéziel H.: PL44, a cosmid containing microsatellite INRA242 (DXS39), maps to bovine chromosome Xq25. Anim. Genet. 1999, 30, s. 161.
• 6. Léotard D., Kirsch-Volders M., Alexandre H., Poma K., Susanne C.: Relationship between frequencies of univalents and meiotic segregation on different mouse strains and their hybrids. Genetica 1987, 75, 189-198.
• 7. Kałużewski B.: Czy chromosomy płciowe są nam potrzebne? Kosmos 1994, 43, 371-388.
• 8. Krzanowska H.: X-Y chromosome dissociation in mouse strains differing in efficiency of spermatogenesis: Elevated frequency of univalents in pubertal males. Gamete Res. 1989, 23, 356-357.
• 9. Pinkel D., Gray J. W., Trask B., van den Engh G., Fuscoe J., Van Dekken H.: Cytogenetic analysis by in situ hybridization with fluorescently labelled nucleic acid probes. Proc. Cold Spring Harbor Symposia on Quantitative Biology, Cold Spring Harbor Laboratory 1986, 1, 151-157.
• 10. Rejduch B., Słota E., Gustavsson I.: 60,XY/60,XX chimerism in the germ cell line of mature bulls born in heterosexual twinning. Theriogenology 2000, 54, 621-627.
• 11. Rejduch B.: Zjawisko chimeryzmu komórkowego XX/XY u zwierząt z rodziny Bovidae. Rocz. Nauk. Zoot. Rozprawy habilitacyjne, Instytut Zootechniki, Kraków 2001, 14.
• 12. Rejduch B., Kozubska-Sobociñska A., Słota E.: Wykorzystanie bydlęcej sondy malującej do identyfikacji heterosomu Y w plemnikach tryka. Rocz. Nauk. Zoot. 2005, 32, 5-9.
• 13. Świtoński M., Stranzinger G.: Studies of synaptonemal complexes in farm mammals - a review. J. Hered. 1998, 89, 473-480.
• 14. Villagomez D. A. F.: Zygotene-pachytene substaging and synaptonemal complex karyotyping of boar spermatocytes. Hereditas 1993, 118, 87-99.