Dicarboxylate platinum [II] complexes as inhibitors of plasma membrane Hplus-ATPase in the yeast Saccharomyces cerevisiae

• Autorzy: Oblak E , Lachowicz T.M. , Waszkiewicz K. , Kuduk-Jaworska J.
• Języki publikacji: EN

Abstrakty

EN

A series of cytotoxic neutral dicarboxylatoplatinum(II) complexes containing D(+), L(-) or DL-malate dianion and ethylenediamine or 1-ethylimidazole as ligands were examined using ATPase activity assays and the proton extrusion test. ATPase activity assays in vitro on plasma membrane H+- ATPase and on mitochondrial ATPase were carried out. The concentrations of compounds inhibiting enzyme activity to 50 per cent (J50) was determined. The new platinum complexes showed a stronger level of inhibition of both ATPases than the reference carboplatin; this inhibitory activity is related to a stereoisomeric form of anionic platinum ligands. ATPase inhibition in vivo was tested by glucose-stimulated proton extrusion and the influence of platinum compounds on this process in yeast cells was determined. Significant differences in activity levels were observed between those complexes with 1-ethylimidazole and those with ethylenediamine.

Słowa kluczowe

EN

yeast; drug resistance; plasma membrane; anticancer drug; cytotoxic platinum; carboplatin; dicarboxylate platinum; cisplatin; ATPase; platinum complex; inhibitor; Saccharomyces cerevisiae

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2003
• Tom: 08
• Numer: 4
• Opis fizyczny: p.1005-1011,fig.

Twórcy

autor

Oblak E

• University of Wroclaw, Przybyszewskiego 65-73, 51-148 Wroclaw, Poland

autor

Lachowicz T.M.

autor

Waszkiewicz K.

autor

Kuduk-Jaworska J.

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