Isoglucose production from raw starchy materials based on a two-stage enzymatic system

• Autorzy: Gromada A , Fiedurek J. , Szczodrak J.
• Języki publikacji: EN

Abstrakty

EN

A new low-cost glucoamylase preparation for liquefaction and saccharification of starchy raw materials in a one-stage system was developed and characterized. A non-purified biocatalyst with a glucoamylase activity of 3.11 U/mg, an α-amylase activity of 0.12 WU/mg and a protein content of 0.04 mg protein/mg was obtained from a shaken-flask culture of the strain Aspergillus niger C-IV-4. Factors influencing the enzymatic hydrolysis of starchy materials such as reaction time, temperature and enzyme and substrate concentration were standardized to maximize the yield of glucose syrup. Thus, a 90% conversion of 5% starch, a 67.5% conversion of 5% potato flour and a 55% conversion of 5% wheat flour to sweet syrups containing up to 87% glucose was reached in 3 h using 1.24 glucoamylase U/mg hydrolyzed substrate. The application of such glucoamylase preparation and a commercially immobilized glucose isomerase for the production of glucose-fructose syrup in a two-stage system resulted in high production of stable glucose/fructose blends with a fructose content of 50%. A high concentration of fructose in obtained sweet syrups was achieved when isomerization was perfonned both in a batch and repeated batch process.

Słowa kluczowe

EN

starch; isomerization; raw material; glucose-fructose syrup; glucoamylase preparation; isoglucose production; Aspergillus niger; enzyme technology; glucoamylase

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2008
• Tom: 57
• Numer: 2
• Opis fizyczny: p.141-148,fig.,ref.

Twórcy

autor

Gromada A

• Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland

autor

Fiedurek J.

autor

Szczodrak J.

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