Molecular cloning, expression and characterization of Bmserpin-2 gene from Bombyx mori

• Autorzy: Pan Y. , Xia H. , Lu P. , Chen K. , Yao Q. , Chen H. , Gao L. , He Y. , Wang L.
• Języki publikacji: EN

Abstrakty

EN

Serpins are a broadly distributed family of protease inhibitors. In this study, the gene encoding Bombyx mori serpin-2 (Bmserpin-2) was cloned and expressed in E. coli. The Bmserpin-2 cDNA contains a 1125 bp open reading frame (ORF). The deduced protein has 374 amino-acid residues, contains a conserved SERPIN domain and shares extensive homology with other invertebrate serpins. RT-PCR analysis showed that Bmserpin-2 was expressed in all developmental stages of B. mori larvae and various larval tissues. Subcellular localization analysis indicated that Bmserpin-2 protein was located in the cytoplasm. Interestingly, real-time quantitative PCR revealed that the expression of Bmserpin-2 in the midgut of susceptible B. mori strain 306 significantly increased at 72 hours post inoculation (hpi) when infected with BmNPV. However, there was no significant increase of the Bmserpin-2 expression in resistant strain NB infected with BmNPV. Thus, our data indicates that Bmserpin-2 may be involved in B. mori antiviral response.

Słowa kluczowe

EN

gene expression; serine protease inhibitor; molecular cloning; serpin; Bmserpin-2 gene; subcellular location; bioinformatics; Bombyx mori; serpin-2

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2009
• Tom: 56
• Numer: 4
• Strony: 671-677
• Opis fizyczny: p.671-677,fig.,ref.

Twórcy

autor

Pan Y.

• Jiangsu University, 301 Xuefu Road, Zhenjiang 212013, P.R.China

autor

Xia H.

autor

Lu P.

autor

Chen K.

autor

Yao Q.

autor

Chen H.

autor

Gao L.

autor

He Y.

autor

Wang L.

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