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Liczba wyników
2007 | 63 | 06 | 655-657
Tytuł artykułu

Evaluation of agreement of ELISA and complement fixation test in the diagnostics of Q fever in cattle

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Q fever is a worldwide zoonosis that is manifested as a reproductive failure in animals and by polymorphic, nonspecific symptoms in humans. The infection may be acquired through the respiratory or alimentary route or an arthropod bite. The diagnosis of Q fever relies mainly upon serology: indirect immunofluorescence assays (IFA), complement fixation (CF) tests or enzyme-linked immunosorbent assays (ELISA) are used to detect antibodies against Coxiella burnetii in sera of infected animals. The aim of our study was to evaluate the agreement of the commercially available ELISA kit and CF test using statistical methods (Kappa value). We used serum samples that were collected from 122 dairy cows from one herd in central Poland. The general health status of the herd was good, and the animals were clinically normal. Our results showed a low agreement (Kappa value = 0.376) between the commercially available ELISA and CF test.
Wydawca
-
Rocznik
Tom
63
Numer
06
Strony
655-657
Opis fizyczny
p.655-657,ref.
Twórcy
autor
  • Warsaw Agricultural University, Nowoursynowska 159C, 02-776 Warsaw, Poland
autor
autor
autor
autor
autor
Bibliografia
  • 1. Arricau-Bouvery N., Rodolakis A.: Is Q Fever an emerging or re-emerging zoonosis? Vet. Res. 2005, 36, 327-349.
  • 2. Field P. R., Santiago A., Chan S. W., Patel D. B., Dickeson D., Mitchell J. L., Devine P. L., Murphy A. M.: Evaluation of a novel commercial enzyme-linked immunosorbent assay detecting Coxiella burnetii-specific immunoglobulin G for Q fever prevaccination screening and diagnosis. J. Clin. Microbiol. 2002, 40, 3526-3529.
  • 3. Field P. R., Mitchell J. L., Santiago A., Dickeson D., Chan S. W., Ho D. W., Murphy A. M, Cuzzubbo A. J., Devine P. L.: Comparison of a commercial enzyme-linked immunosorbent assay with immunofluorescence and complement fixation tests for detection of Coxiella burnetii (Q fever) immunoglobulin M. J. Clin. Microbiol. 2000, 38, 1645-1647.
  • 4. Foley J. E., Biberstein E. L., Hirsh D. C.: Rickettsiae: Rickettsia, Coxiella and Orientia, [in:] Hirsh D. C. (ed.): Veterinary Microbiology. Blackwell Publishing, Oxford 2004.
  • 5. Fournier P. E., Marrie T. J., Raoult D.: Diagnosis of Q fever. J. Clin. Microbiol. 1998, 36, 1823-1834.
  • 6. Kim S. G., Kim E. H., Lafferty C. J., Dubovi E.: Coxiella burnetii in bulk tank milk samples, United States. Emerg. Infect. Diseas. 2005, 11, 619-621.
  • 7. Kloppert B., Wolter W., Zschock M., Kabisch D., Hamann H. P., Frost J. W.: Coxiella burnetii as zoonotic pathogen with special regard to food hygiene. Dtsch. Tierärztl. Wschr. 2004, 111, 321-323.
  • 8. Peter O., Dupuis G., Bee D., Luthy R., Nicolet J., Burgdorfer W.: Enzyme-linked immunosorbent assay for diagnosis of chronic Q fever. J. Clin. Microbiol. 1988, 26, 1978-1982.
  • 9. Slaba K., Skultety L., Toman R.: Efficiency of various serological techniques for diagnosing Coxiella burnetii infection. Acta Virol. 2005, 49, 123-127.
  • 10. Stein A., Raoult D.: Detection od Coxiella burnetii by DNA amplification using polymerase chain reaction. J. Clin. Microbiol. 1992, 30, 2462-2466.
  • 11. Sting R., Breitling N., Oehme R., Kimmig P.: The occurrence of Coxiella burnetii in sheep and ticks of the genus Dermacentor in Baden-Wirttemberg. Dtsch. Tierärztl. Wochenschr. 2004, 111, 390-394.
  • 12. Thrusfield M.: Veterinary epidemiology. Blackwell Science, Oxford 2005, 305-330.
  • 13. Willems H., Thiele D., Frolich-Ritter R., Krauss H.: Detection of Coxiella burnetii in cow's milk using the polymerase chain reaction. J. Vet. Med. B 1994, 41, 580-587.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-edc8547a-6093-4297-bdfe-04932859595e
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