Occurrence of Toxoplasma gondii in water from wells located on farms

• Autorzy: Sroka J. , Wojcik-Fatla A. , Dutkiewicz J.
• Języki publikacji: EN

Abstrakty

EN

Among various species of parasitic protozoans which may contaminate drinking water, Toxoplasma gondii is of a special importance due to the high incidence of infections with this parasite noted in animals and humans. The objective of this study was to determine the frequency of occurrence of T. gondii in drinking water on farms in the area of the Lublin province (eastern Poland) with respect to health risk among the inhabitants, and to assess the role of water in the transmission of Toxoplasma infections in the rural environment. Studies were conducted on 87 farms located in the Lublin province, 14 of which were classified as possessing a good hygienic state, and 73 as possessing a poor hygienic state. A total number of 114 drinking water samples were taken, 80 samples from shallow household wells with a windlass, 16 from deep wells with a pump, and 18 from the water supply system. In microscopic and PCR examinations of 114 water samples, T. gondii was found in 15 (13.2%) and 31 (27.2%) of samples, respectively. The presence of T. gondii DNA detected by PCR test was found significantly more frequently in water samples from the shallow windlassoperated wells than in those from deep wells (p<0.05) and water supply system (p<0.01). Water samples collected from shallow wells located on farms of poor hygienic state contained significantly more frequently DNA of T. gondii than samples from shallow wells located on farms of good hygienic state (43.1% vs. 13.3%, p<0.05). In 26.3% of water samples, oocysts of other protozoans were found belonging to Isospora, Eimeria, and Cryptosporidium. Serologic examinations for the presence of anti-Toxoplasma antibodies conducted among 99 inhabitants of the farms where household wells were used showed 64.6% of seropositive results in IgG class antibodies and 1.0% in IgM class antibodies. Clinical cases of toxoplasmosis were also noted. In the total population examined, a positive correlation was observed between the consumption of unboiled well water and the presence of antibodies against T. gondii (p<0.05), this correlation being especially strong on farms of poor hygienic state enclosing shallow wells (p<0.001). In conclusion, the recorded presence of T. gondii in well water provides an evidence of the potential risk of waterborne infection for humans and animals. Therefore, it seems necessary to implement prophylactic actions on the endangered farms.

Słowa kluczowe

EN

toxoplasmosis; water-borne zoonosis; human disease; serological test; Toxoplasma gondii; polymerase chain reaction; farmer; Lublin region; oocyst

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2006
• Tom: 13
• Numer: 1
• Opis fizyczny: p.169-175,fig.,ref.

Twórcy

autor

Sroka J.

• Institute of Agricultural Medicine, P.O.Box 185, 20-950 Lublin, Poland

autor

Wojcik-Fatla A.

autor

Dutkiewicz J.

Bibliografia

• 1. Bahia-Oliveira LMG, Jones JL, Azevedo-Silva J, Alves CCF, Orefice F, Addiss DG: Highly endemic, waterborne toxoplasmosis in North Rio de Janeiro State, Brazil. Emerg Infect Dis 2003, 9, 55-62.
• 2. Benenson MW, Takafuji ET, Lemon SM, Greenup RL, Sulzer AJ: Oocyst-transmitted toxoplasmosis associated with ingestion of contaminated water. AT Engl J Med 1982, 307, 666-669.
• 3. Bowie WR, King AS, Werker DH, Isaac-Renton JL, Bell A, Eng SB, Marion SA: Outbreak of toxoplasmosis associated with municipal drinking water. The BC Toxoplasma Investigation Team. Lancet 1997, 350, 173-177.
• 4. Dawson D: Foodborne protozoan parasites. Int J Food Microbiol 2005,103, 207-227.
• 5. Dubey JP, Beattie CP: Toxoplasmosis of Animals and Man. CRC Press Inc., Boca Raton 1988.
• 6. Ertug S, Okyay P, Turkmen M, Yuksel H: Seroprevalence and risk factors for Toxoplasma infection among pregnant women in Ay din province, Turkey. BMC Public Health 2005, 5, 66.
• 7. Hall SM, Pandit A, Golwilkar A, Williams TS: How do Jains get Toxoplasma infection? Lancet 1999, 354, 486-487.
• 8. Isaac-Renton J, Bowie WR, King A, Irwin GS, Ong CS, Fung CP, Shokeir MO, Dubey JP: Detection of Toxoplasma gondii oocysts in drinking water. Appl Environ Microbiol 1998, 64, 2278-2280.
• 9. Kellogg J, McDevitt S, McDevitt JJ: Development of a PCR- Enzyme Immunoassay Oligoprobe Detection Method for Toxoplasma gondii oocysts, incorporating PCR controls. Appl Environ Microbiol 2003,69,5819-5825.
• 10. Kourenti C, Heckeroth A, Tenter A, Karanis P: Development and application of different methods for the detection of Toxoplasma gondii in water. Appl Environ Microbiol 2003, 69, 102-106.
• 11. Lopez-Castillo CA, Diaz-Ramirez J, Gomez-Marin JE: Risk factors for Toxoplasma gondii infection in pregnant women in Armenia, Colombia. Rev Salud Publica 2005, 7, 180-90.
• 12. Majewska AC, Kasprzak W: Wodnopochodne epidemie Giardia. Wiad Parazytol 1995, 41, 25-31.
• 13. Majewska AC: Pasożytnicze pierwotniaki w wodzie. MNil. Przegląd Eureka 2003, 8.
• 14. Paul M: Potencjalne źródła zarażenia Toxoplasma gondii w przypadkach badanych w krótkim czasie po zarażeniu. Przegl Epidemiol 1998, 52, 447-454.
• 15. Pozio E: Foodborne and waterborne parasites. Acta Microbiol Pol 2003, 52, Suppl., 83-96.
• 16. Siński E: Environmental contamination with protozoan parasite infective stages: biology and risk assessment. Acta Microbiol Pol 2003, 52, Suppl., 97-107.
• 17. Sroka J: Badania nad występowaniem Toxoplasma gondii u zwierząt hodowlanych i dzikich z terenu województwa lubelskiego w aspekcie zagrożenia zdrowia ludności wiejskiej. Doctoral Thesis. The Faculty of Veterinary Medicine, Agricultural University of Lublin, Lublin 2005.
• 18. Sroka J: Seroepidemiology of toxoplasmosis in the Lublin region. Ann Agric Environ Med 2001, 8, 25-31.
• 19. Tenter AM, Heckeroth AR, Weiss LM: Toxoplasma gondii: from animals to humans. Int J Parasitol 2000, 30, 1217-1258.
• 20. Villena I, Aubert D, Gomis P, Ferté H, Inglard JC, Denis-Bisiaux H, Dondon JM, Pisano E, Ortis N, Pinon JM: Evaluation of a strategy for Toxoplasma gondii oocyst detection in water. Appl Environ Microbiol 2004, 70, 4035-4039.