Vitamin A deficiency alters the bioelectric parameters and RNA content of rat gastric mucosa in vitro

• Autorzy: Ventura U. , Ceriani T. , Montini E. , Romano M. , Ricci V.
• Języki publikacji: EN

Abstrakty

EN

The present study was aimed to investigate the mechanisms by which vitamin A plays a role in maintaining the efficiency of gastric mucosal barrier. Particularly, we measured electrical parameters and the RNA/DNA ratio of gastric mucosa isolated in vitro from the stomach of rats in which vitamin A-deficiency was induced by means of a vitamin A-free diet and then abolished by means of a massive vitamin A supplementation. Pair-fed vitamin A-nondepleted rats and normal rats fed ad libitum on a standard diet served as controls. Vitamin A status was assayed for each group of rats by measuring the hepatic content of vitamin A. We found that in gastric mucosa vitamin A-deficiency induced: 1) a decrease in both transmucosal potential difference and short-circuit current; 2) an increase in transmucosal electrical resistance; 3) a decrease in RNA content resulting in a decreased RNA/DNA ratio. Abolishment of vitamin A-deficiency restored both electrical parameters and RNA content of rat gastric mucosa. Our results stress the role of vitamin A in maintaining the efficiency of the gastric mucosal barrier. Vitamin A seems to act by stabilizing gastric electrical parameters and by controlling the protein synthesis/turnover in the surface gastric mucosal cells.

Słowa kluczowe

EN

vitamin A; bioelectrical parameter; gastric mucosa; DNA; in vitro; rat; RNA content

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2003
• Tom: 54
• Numer: 4
• Opis fizyczny: p.523-532,fig.,ref.

Twórcy

autor

Ventura U.

• University of Pavia, Via Forlanini 6, I-27100 Pavia, Italy

autor

Ceriani T.

autor

Montini E.

autor

Romano M.

autor

Ricci V.