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Czasopismo
2004 | 63 | 2 |
Tytuł artykułu

The detection of Helicobacter pylori in paraffin sections using the PCR technique and various primers as compared to histological techniques

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Helicobacter pylori is thought to represent a significant etiopathogenic factor in diseases of the upper gastrointestinal tract. It seems, therefore, important to elaborate effective techniques for its detection. The aim of the present study was to evaluate the effectiveness of Helicobacter pylori detection using the PCR technique on paraffin sections with various pairs of primers and to compare the results with those of a histological appraisal. Material for the studies involved 50 paraffin blocks with gastric mucosa biopsies fixed in 4% buffered formalin. In this material 4 tests were performed with the aim of diagnosing Helicobacter pylori infection: 1) H+E staining, 2) staining by the Giemsa technique, 3) an immunocytochemical technique with antibodies against H. pylori and 4) the PCR technique with various primers. In the present study the most reliable results for H. pylori detection as well as the most pronounced correlation were obtained by using the PCR technique with primers for the ureC gene, immunohistochemistry and staining according to Giemsa. Less compatible results were obtained employing the two PCR techniques which utilise various primers. The experiments confirmed the usefulness of the PCR technique in the detection of Helicobacter pylori in paraffin sections by using a suitable pair of primers, and also indicated that Giemsa staining and immunohistochemistry should be taken into account.
Wydawca
-
Czasopismo
Rocznik
Tom
63
Numer
2
Opis fizyczny
p.229-231,fig.,ref.
Twórcy
autor
  • Medical University, Chalubinskiego 6a, 50-368 Wroclaw, Poland
autor
autor
autor
Bibliografia
  • 1. Andersen LP (2001) Pathogenesis and host response in Helicobacter infection. FEMS Immunol Med Microbiol, 30: 85.
  • 2. Goosen C, Theron J, Ntsala M, Maree FF, Olckers A, Botha SJ, Lastovica AJ, van der Merwe SW (2002) Evaluation of a novel heminested PCR assay based on the phosphoglucosamine mutase gene for detection of H. pylori in saliva and dental plaque. J Clin Microbiol, 40: 205–209.
  • 3. Gościniak G (2002) Presence of Helicobacter pylori antigens in stools of children with chronic gastritis (in Polish). Pol Merk Lek, 12, 68: 104–107.
  • 4. Lu J-J, Perng Ch-L, Shyu R-Y, Chen Ch-H, Lou Q, Chong SK, Lee CH-H (1999) Comparison of five PCR methods for detection of Helicobacter pylori DNA in gastric tissues. J Clin Microbiol, 37: 772–774.
  • 5. Megraud F (1997) How should Helicobacter infection be diagnosed? Gastroenterology, 112: 93–98.
  • 6. Roosendaal R, Kuipers EJ, van der Brule AJC, Pena AS, Uyterlinde AM, Walboomers LMM, Meuwissen SGM, de Graaf J (1994) Importance of the fiberoptic endoscope cleaning procedure for detection of Helicobacter pylori in gastric biopsy specimens by PCR. J Clin Microbiol, 32: 1123–1126.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-eb69135a-d64b-46be-82a5-e668c7b26217
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