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The purpose of the presented study was the analysis of post-ethanol fibrogenesis in the pancreas, being one of the causes of exo- and endocrine pancreatic insufficiency. The study was performed on rats classified into a control and experimental group. The animals from the experimental group were given 20% ethyl alcohol solution ad libitum for 4 weeks. After decapitation of the animals, pancreatic segments were collected and histological sections of the segments stained with haematoxylin and eosin and with Masson's method for connective tissue were prepared. In the sections from the experimental group, a significant increase in the number of connective tissue fibres was observed. They were observed not only around the lumen of glandular ducts (as it was the case in the control group), but also among some vesicles and on the border of the pancreatic lobules. Their accumulation was also observed around the cells forming the pancreatic islets.
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p.449-451,fig.,ref.
Twórcy
autor
- Medical University of Lublin, 20-080 Lublin, Poland
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Bibliografia
- 1. Apte M.V., Pirola R.C., Wilson J.S.: Battle-scarred pancreas: role of alcohol and pancreatic stellate cells in pancreatic fibrosis. J Gastroenterol Hepatol 2006, Suppl 3, 97-101.
- 2. Conde de la Rosa L., Moshage H., Nieto N.: Hepatocyte oxidant stress and alcoholic liver disease. Rev Esp Enferm Dig 2008, 100, 156-163.
- 3. De Minicis S., Brenner D.A.: Oxidative stress in alcoholic liver disease: role of NADPH oxidase complex. J Gastroenterol Hepatol 2008, 23, S98-S103.
- 4. Den X., Wang L., Elm M.S., Gabazadeh D., Diorio G.J., Eagon P.K., Whitcomb D.C.: Chronic alcohol consumption accelerates fibrosis in response to cerulein - induced pancreatitis in rats. Am J Pathol 2005, 166, 93- 106.
- 5. Gukovsky I., Lugea A., Shahsahebi M., Cheng J.H., Hong P.P., Jung Y.J., Deng Q.G., French B.A., Lungo W., French S.W., Tsukamoto H., Pandol S.J.: A rat model reproducing key pathological responses of alcoholic chronic pancreatitis. Am J Physiol Gastrointest Liver Physiol 2008, 294, G68-G79.
- 6. Jaster R., Emmrich J.: Crucial role of fibrogenesis in pancreatic diseases. Best Pract Res Clin Gastroenterol 2008, 22, 17-29.
- 7. Masamune A., Kikuta K., Satoh M., Satoh A., Shimosegawa T.: Alcohol activated activator protein-1 and mitogen-activated protein kinases in rat pancreatic stellate cells. J Pharmacol Exp Ther 2002, 302, 36-42.
- 8. Stasiewicz J.: Podstawy patogenetyczne leczenia chorób przewodu pokarmowego. Ed. PZWL, Warszawa, 1992.
- 9. Uesugi T., Froh M., Gabele E., Isayama F., Bradford B.U., Ikai I., Yamaoka Y., Arteel G.E.: Contribution of angiotensin II to alcohol-induced pancreatic fibrosis in rats. J Pharmacol Exp Ther 2004, 311, 921-928.
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Bibliografia
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bwmeta1.element.agro-article-e85f6d3c-2e36-4065-81a7-fcd8b95f2a73