Structural polymorphism of telomeres studied by photon correlation spectroscopy

• Autorzy: Wlodarczyk A. , Gapinski J. , Patkowski A. , Dobek A.
• Języki publikacji: EN

Abstrakty

EN

Photon Correlation Spectroscopy (PCS) was used to study the dynamics and structure of Tetrahymena telomeric sequence d(5'-TGGGGT-3')4. Two different modes were observed, corresponding to the following structures: intermolecular (tetramolecular) G-quadruplex and intramolecular (monomeric) G-quartet. Experimental values of translational diffusion coefficients DT were obtained for each structural form. The value of DT for the monomer equals to 1.4 × 106(cm2/s), while for the tetramolecular structure, to 0.8 × 106(cm2/s). The relative weight concentrations of these two forms were analyzed versus the concentration of NaCl varied from 10 mM to 500 mM. The values of experimentally determined diffusion coefficients were compared with those calculated assuming the "bead model" and with the atomic coordinates from the NMR and X-ray crystallographic data. For both structures the experimental and calculated values of DT were in reasonable agreement. In the entire NaCl concentration range studied, the contribution of the relative weight concentration of the monomeric telomere form changed from 85% for 10 mM NaCl to 60% for 500 mM NaCl.

Słowa kluczowe

EN

DNA structure; polymorphism; photon correlation spectroscopy; telomere; Tetrahymena; eukaryotic chromosome

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 3
• Opis fizyczny: p.609-613,fig.

Twórcy

autor

Wlodarczyk A.

• A.Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland

autor

Gapinski J.

autor

Patkowski A.

autor

Dobek A.

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