Wystepowanie i charakterystyka szczepow opornych na antybiotyki oksy-imino-betalaktamowe wsrod szczepow Salmonella enterica subsp. enterica izolowanych w Polsce

• Autorzy: Szych J. , Gierczynski R. , Wardak S. , Cieslik A.

Warianty tytułu

EN

The occurrence and characterisation of oxy-imino-beta-lactams resistant strains among Salmonella enterica subsp. enterica isolated in Poland

• Języki publikacji: PL

Abstrakty

PL

Scharakteryzowano 68 szczepów pałeczek Salmonella opornych na antybiotyki oksy-imino-betalaktamowe należących do 6 typów serologicznych, wybranych spośród 239 opornych na ampicylinę szczepów pałeczek Salmonella izolowanych w Polsce w latach 1999-2004 z próbek materialu klinicznego. Stwierdzono, że większość badanych szczepów wytwarzała p-Iaktamazę TEM-1 o pl=5,4 oraz p-laktamazę o rozszerzonym spektrum substratowym CTX-M-3 o pl=8,4. Wszystkie badane szczepy S. Thompson i jeden ze szczepów S. Typhimurium wytwarzał enzym SHV-5 o pl=8,2. Jest to pierwsze doniesienie o produkcji SHV-5 przez pałeczki Salmonella w Polsce.

EN

Extended - spectrum ß-lactamases (ESBLs) are enzymes manifesting considerable hydrolyzing activity on a wide variety of ß-lactam antibiotics including oxyiminocephalosporins and aztreonam. In the study reported here we investigated the types of ESBL produced by Salmonella enterica subsp. enterica strains isolated from clinical samples in the microbiological laboratories of sanitary-epidemiological units in Poland from 1999 to 2004. Among 239 ampicilline-resistant Salmonella enterica subsp. enterica strains isolated from clinical samples in the microbiological laboratories of sanitary-epidemiological units in Poland, 68 isolates of oximino-beta-lactams resistant of 6 serovars were found. There were 16 epidemiological unrelated strains (6 isolates of S.Enteritids, S isolates of S.Thompson, 3 isolates of S.Typhimurium, onefold isolate of S.Muenster and S. enterica 1,9,12:-:-) coming from different areas of country and 52 epidemiologically related isolates of S. Oranienburg, coming from a prolonged outbreak in an orphanage in Łódź. All the strains were identified as the ESBLs producers . The molecular analysis relevead that most of them expressed CTX-M-3 ESBL which is widely observed in Poland and additional enzyme TEM-1. All tested isolates of S.Thompson and one of three S. Typhimurium isolates were found to produce SHV-5 ESBL. This is the first report regarding the presence of SHV-5 in the genus Salmonella in Poland.

Słowa kluczowe

PL

Salmonella enterica subsp.enterica; Polska; wystepowanie; szczepy bakteryjne; izolacja; opornosc na antybiotyki; ampicylina; antybiotyki oksy-imino-beta-laktamowe; beta-laktamaza; lancuchowa reakcja polimerazy; analiza sekwencyjna

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2005
• Tom: 57
• Numer: 2
• Strony: 115-130
• Opis fizyczny: s.115-130,rys.,tab.,bibliogr.

Twórcy

autor

Szych J.

• Panstwowy Zaklad Higieny w Warszawie, ul.Chocimska 24, 00-791 Warszawa

autor

Gierczynski R.

autor

Wardak S.

autor

Cieslik A.

Bibliografia

• 1. Baraniak A, Fiett J, Sulikowska A, Hryniewicz W, Gniadkowski M. Countrywide spread of CTX- М-3 extended-spectrum ß-lactamase (ESBL)-producing microorganism of the family Enterobacteriaceae in Poland. Antimicrob Agents Chemother 2002; 46: 151-9.
• 2. Baraniak A, Sadowy E, Hryniewicz W, Gniadkowski M. Two dilferent extended spectrum b-lactamases (ESBLs) in One of the first ESBL-producing Salmonella isolates in Poland. J Clin Microbiol 2002; 40: 1095-7.
• 3. Barnaud G, Arlet G, Verdet C i wsp. Salmonella enteritidis: AmpC plasmid-mediated inducible b- lactamase (DHA-1) with an ampR gene from Morganella morganii. Antimicrob Agents Chemother. 1998; 42: 2352-8.
• 4. Bauernfeind A, Stemplinger I, Jungwirth R і wsp. Characterization of b-lactamase gene blaPER-2 which encodes an extended spectrum class A ß-lactamase. Antimicrob Agents Chemother 1996; 40: 616-20.
• 5. Bradford PA, Yang Y, Sahm D і wsp. CTX-M-5, a novel cefotaxime-hydrolyzing ß-lactamase from an outbreak of Salmonella typhimurium in Latvia. Antimicrob Agents Chemother 1998; 42: 1980-84.
• 6. Choroby zakaźne i zatrucia w Polsce w 2003 roku. Państwowy Zakład Higieny, Zakład Epidemiologii, Główny Inspektor Sanitarny, Warszawa 2004 www.pzh.gov.pl/epimeld/index_p.html
• 7. Gierczyński R, Szych, J Rastawicki W, Jagielski M. The molecular characterisation of the extended spectrum ß-lactamase (ESBL) producing strain of Salmonella enterica serovar Mbandaka isolated in Poland. Acta Microbiol Polonica 2003; 52: 183-90.
• 8. Gierczyński R, Szych J, Rastawicki W, Jagielski M. The occurance of the first two CTX-M-3 and ТЕМІ producing isolates of Salmonella enterica serovar Oranienburg in Poland. Int J Antimicrob Agents; 2003: 498-9.
• 9. Fisher I ST і wsp. International trends in Salmonella serotypes 1998-2003 - a surveillance report from the Enter-net International Surveillance Network. Eurosurveillance 2004; 9: 45-7.
• 10. GniadkowskiM, Schneider I, Jungwirth R і wsp. Ceftazidime-resistant Enterobacteriaceae isolates from three polish hospital: Identification of three novel ТЕМ- and SHV-5-type extended-spectrum b-lactamases Antimicrob Agents Chemother 1998; 42: 514-20.
• 11. Gniadkowski M, Schneider I, Palucha A i wsp. Cefotaxime-resistant Enterobacteriaceae isolates from a hospital in Warsaw, Poland: Identification of a new CTX-M-3 cefotaxime-hydrolysing ß-lactamase that is closely related to the CTX-M-l/MEN-1 enzyme. Antimicrob Agents Chemother 1998; 42: 827-32.
• 12. Hryniewicz W, Sulikowska A, Szczypa K, Krzysztoń-Russjan J i wsp. Rekomendacje doboru testów do oznaczania wrażliwości bakterii na antybiotyki i chemioterapeutyki. Mikrobiologia Medycyna 2004; 40: 3-28.
• 13. Liebana E, Batchelor M, Torres C, Brińas L i wsp. Pediatrie infection due to multiresistant Salmonella enterica serotype Infantis in Honduras. J Clin Microb 2004; 42: 4885-8.
• 14. Livermore DM. ß-Lactamases in laboratory and clinical resistance. Clin Microb Rev. 1995; 8: 557-84.
• 15. Miriagou V, Filip R, Coman G, Tzouvelekis LS. Expanded-spectrum cephalosporin-resistant Salmonella strains in Romania. J Clin Microb 2002; 40: 4334-6.
• 16. Miriagou V, Tassios PT.Legakis NJ, Tzouvelekis LS. Expanded-spectrum cephalosporin resistance in non-typhoid Salmonella Int J Antimicrob Agents. 2004; 23: 547-55.
• 17. National Committee for Clinical Laboratory Standards. Performance Standards for antimicrobial susceptibility testing; Eleventh informational supplement NCCLS, 2001.
• 18. Naumiuk Ł, Baraniak A, Gniadkowski M i wsp. Molecular epidemiology of Serratia marcescens in two hospitals in Danzig, Poland, over a 5-year period. J Clin Microbiol 2004; 42: 3108-16.
• 19. Payne DJ, Farmer TH. Biochemical and enzyme kinetic applications for the characterization of ß-lactamases w Methods in Molecular Medicine, Vol. 15: Molecular Bacteriology: Protocols and Clinical Applications Ed Woodford N, Johnson AP © Humana Press Inc., Totowa, NJ.
• 20. Revathi G, Shannon KP, Stapleton PD i wsp. An outbrea of extended-spectrum, ß-lactamase - producing Salmonella senftenberg in a burns ward. J Hosp Inf 1998; 40: 295-302.
• 21. Sambrook J, Fritsch E F, Maniatis T. Molecular cloning: a laboratory manual, 2nd ed. New York: Cold Spring Harbor Laboratory Press, 1989.
• 22. Szych J, Cieślik A, Paciorek J, Kałużewski S. Antibiotic resistance in Salmonella enterica subsp enterica strains isolated in Poland from 1998-1999. Int J Antimicrob Agents 2001,18:37-42.
• 23. Thompson KS. Controversis about extended-spectrum and AmpC beta-lactamases. Emerging Inf Dis. 2001;7:333-6.
• 24. Weldhagen GF. Integrons and ß-lactamases - a novel perspective on resistance. Int J Antimicrob Agents 2004; 23: 556-62.
• 25. Wu TL, Siu K, Su LH і wsp. Outer membrane protein change combined with co-existing TEM-1 and SHV-1 ß-lactamases lead to false identification of ESBL-producing Klebsiella pneumoniae. J Antimicrob Chemother 2001;47:755-61.
• 26. Van JJ, Ko WC, Chiu CH i wsp. Emergence of ceftriaxone-resistat Salmonella isolates and rapid spread of plasmid-encoded CMY-2- like cephalosporinase, Taiwan. Emerging Inf Dis, 2003; 9: 323-8.