Genetic structure and phylogenetic relationships of the Polish Heavy horse

• Autorzy: Iwanczyk E , Juras R , Cholewinski G , Cothran E G
• Języki publikacji: EN

Abstrakty

EN

In this study a wide range of genetic markers (12 microsatellites, 7 blood-group loci, 10 blood-protein loci) and mitochondrial DNA (mtDNA) were used to assess genetic diversity in Polish Heavy horses. Three random samples were sequenced for 421 bp of the mitochondrial D-loop region, but no clear phylogenetic patterns were seen in mtDNA variation. Both heterozygosity and diversity levels are fairly high in Polish Heavy horses. In phylogenetic analysis the draught horses form a distinct cluster that pairs with the true pony breeds. Within this ‘cold-blooded’ group, the Polish Heavy Horse clusters most closely with the Posavina breed from Croatia and the Breton breed from France. From the standpoint of genetic conservation, the Polish Heavy Horse does not appear to be in jeopardy.

Słowa kluczowe

EN

animal genetics; horse; genetic structure; genetic marker; genetic variation; blood group; phylogenetic relationship; genetic conservation; Polish Heavy horse; mitochondrial DNA; Equus caballus; horse breed; genetic diversity

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2006
• Tom: 47
• Numer: 4
• Opis fizyczny: p.353-359,fig.,ref.

Twórcy

autor

Iwanczyk E

• Horse Genetic Markers Laboratory, August Cieszkowski Agricultural University of Poznan, Wolynska 33, 60-637 Poznan, Poland

autor

Juras R

• Texas A&M University, College Station, Texas, USA

autor

Cholewinski G

• Horse Genetic Markers Laboratory, August Cieszkowski Agricultural University of Poznan, Wolynska 33, 60-637 Poznan, Poland

autor

Cothran E G

• Texas A&M University, College Station, Texas, USA

Bibliografia

• Aberle KS, Hamann H, Drogemuller C, Distl O, 2004. Genetic diversity in German draught horse breeds compared with the group of primitive, riding and wild horses by means of microsatellite DNA markers. Anim Genet 35: 270-277.
• Andersson L, 1985. The estimation of blood group gene frequencies: a note on the allocation method. Anim Blood Groups Biochem Genet 16: 1-7.
• Binns MM, Holmes NG, Holliman A, Scott AM, 1995. The identification of polymorphic microsatellite loci in the horse and their use in thoroughbred parentage testing. Br Vet J 151: 9-15.
• Bjørnstad G, Gunby E, Roed KH, 2000. Genetic structure of Norwegian horse breeds. J Anim Breed Genet 117: 307-317.
• Bowling AT, Clark RS, 1985. Blood group and protein polymorphism frequencies for seven breeds of horses in the United States. Anim Blood Groups Biochem Genet 16: 93-108.
• Bowling AT, Ryder OA, 1987. Genetic studies of blood markers in Przewalski’s horse. Heredity 78: 75-80.
• Bowling A, Del Valle A, Bowling M, 2000. A pedigree-based study of mitochondrial D-loop DNA sequence variation among Arabian horses. Anim Genet 31: 1-7.
• Braend M, 1973. Genetic variation in equine blood proteins. Karger, Basel: 394-406.
• Braend M, Johansen KE, 1983. Haemoglobin types in Norwegian horses. Anim Blood Groups Biochem Genet 14: 305-307.
• Breen M, Lindgren G, Binns MM, Norman J, Irvin Z, Bell K, et al. 1997. Genetical and physical assignments of equine microsatellites-first integration of anchored markers in horse genome mapping. Mamm Genome 8: 267-273.
• Cann PL, Brown WM, Wilson AC, 1984. Polymorphic sites and the mechanism of evolution in human mitochondrial DNA. Genetics 106: 479-499.
• Cañon J, Checa MI, Carlos C, Vega-Pla JL, Dünner S, 2000. The genetic structure of Spanish horse breeds inferred from microsatellite data. Anim Genet 31: 39-48.
• Cothran EG, Van Dyk E, 1998. Genetic analysis of three South African horse breeds. J S Afr Vet Assoc 69: 120-125.
• Ellegren H, Johansson M, Sandberg K, Andersson L, 1992. Cloning of highly polymorphic microsatellites in the horse. Anim Genet 23: 133-142.
• Felsenstein J, 1989. PHYLIP-Phylogeny inference package (version 3.4). Cladistics 5: 164-166.
• Guérin G, Bertaud M, Amiques Y, 1994. Characterization of seven new horse microsatellites: HMS1, HMS2, HMS3, HMS5, HMS6, HMS7 and HMS8. Anim Genet 25: 62.
• Henney PJ, Johnson EL, Cothran EG, 1994. A new buffer system for acid PAGE typing of equine protease inhibitor. Anim Genet 9: 363-364.
• Hughes SS, 2000. Strand Nucleic Acid Analysis Software. Available: http:/www.vgl.ucdaviess.edu/ STRand. Unversity of California, Davies, CA.
• Ishida N, Hasegawa T, Takeda K, Sakagami M, Onishi A, Inumaru S, et al. 1994. Polymorphic sequence in the D-loop region of the equine mitochondrial DNA. Anim Genet 25: 215-221.
• Jansen T, Forster P, Levine MA, Oelke H, Hurles M, Renfrew C, et al. 2002. Mitochondrial DNA and the origin of the domestic horse. PNAS 99: 10905-10910.
• Juneja R, K, Gahne B, Sandberg K, 1978. Genetic polymorphism of the vitamin D binding protein and other post-albumin protein in horse serum. Anim Blood Grps Biochem Genet 25: 29-36.
• Juras R, Cothran GE, Klimas R, 2003. Genetic analysis of three Lithuanian native horse breeds. Acta Agric Scand, Sec A, Animal Sci 53: 180-185.
• Kavar T, Habe F, Brem G, Dove P, 1999. Mitochondrial D-loop sequence variation among 16 maternal lines of the Lipizzan horse breed. Anim Genet 30: 423-430.
• Marklund S, Ellegren H, Eriksson S, Sandberg K, Andersson L, 1994. Parentage testing and linkage analysis in the horse using a set of highly polymorphic microsatellites. Anim Genet 25: 19-23.
• Nei M, 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583-590.
• Pollitt CC, Bell TK, 1980. Protease inhibitor system in horses: classification and detection of a new allele. Anim Blood Groups Biochem Genet 11: 235-244.
• Sandberg K, 1974. Blood typing of the horse: Current status and application to the identification problems. In: Proceedings of the 1st World Congress of Genetics Applied to Livestock Production. Madrid: 253-265.
• Stormont C, Suzuki Y, 1964. Genetic polymorphism of blood groups in horses. Genetics 50: 915-929.
• Stormont C, Suzuki Y, Rhodes EA, 1964. Serology of horse blood groups. Cornell Vet 54: 439-452.
• Tozaki T, Takezaki N, Hasegawa T, Ishida N, Kurosawa M, Tomita M, et al. 2003. Microsatellite variation in Japanese and Asian horses and their phylogenetic relationship using a European horse outgroup. J Hered 94: 374-380.
• Van Haeringen H, Bowling AT, Scott ML, Lenstra JA, Zwaagstra KA, 1994. A highly polymorphic horse microsatellite locus: VHL20. Anim Genet 25: 207.
• Vila C, Leonard JA, Gotherstrom A, Marklund S, Sandberg K, Linden K, et al. 2001. Widespread origins of domestic horse lineages. Science 291: 474-477.
• Walsh PS, Metzger DA, Higuchi R, 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513.
• Xu X, Arnason U, 1994. The complete mitochondrial DNA sequence of the horse, Equus caballus: extensive heteroplasmy of the control region. Gene 148: 657-662.