Suppression of growth of Fusarium verticillioides Niren. using strains of Trichoderma harzianum from maize [Zea mays] plant parts and its rhizosphere

• Autorzy: Sobowale A A , Odebode A.C. , Cardwell K.F. , Bandyopadhyay R.

Warianty tytułu

PL

Ograniczenie wzrostu Fusarium verticillioides Niren. przy wykorzystaniu szczepow Trichoderma harzianum z czesci roslin kukurydzy [Zea mays] i ich rizosfery

• Języki publikacji: EN

Abstrakty

EN

Three strains of Trichoderma harzianum (strain 1: IMI 380934; strain 2: IMI 380935; strain 3: IMI 380938) were compared for their ability to suppress radial growth of Fusarium verticillioides in vitro. Each Trichoderma strain was paired with the pathogen F. verticillioides on 9 cm Petri plates of acidified potato dextrose agar using three pairing methods. Varying growth suppression of pathogen by Trichoderma strains was rated and ratings were analysed using GLM Procedure of SAS. Growth inhibition of F. verticillioides by each of the T. harzianum strains was significantly different from control irrespective of pairing method (p = 0.01, R2 = 0.96). Higher inhibition of F. verticillioides was obtained by inoculating antagonist before pathogen even at p = 0.01. Mode of suppression includes mycoparasitism and competition for space and nutrients. Growth inhibition of pathogen differed significantly among (p > 0.0001) and within (p > 0.026) pairing methods. T. harzianum strain 1 had better suppression of pathogen than the other two strains when it was inoculated before the pathogen while T. harzianum strain 3 was better when pathogen and antagonist were inoculated simultaneously (p = 0.05). Different strains of T. harzianum could thus be employed as promising antagonists of F. verticillioides.

PL

Porównano trzy szczepy Trichoderma harzianum (szczep 1: IMI 380934; szczep 2: IMI 380935 i szczep 3: IMI 380938) pod względem ich zdolności ograniczania odśrodkowego wzrostu Fusarium verticillioides in vitro. Każdy szczep Trichoderma z zaszczepiono na płytki Petriego o średnicy 9 cm, z zakwaszoną pożywką agarowo-ziemniaczaną, stosując 3 metody. Stosując Procedurę GLM SAS uzyskano zróżnicowane ograniczenie wzrostu patogena przez szczepy Trichoderma. Inhibicja wzrostu prze każdy przez każdy ze szczepów T. harzianum różniła się istotnie od kontroli, niezależnie od wykorzystanej metody (p = 0,01, R2 = 0,96). Wyższą inhibicję F. verticillioides uzyskano w przypadku wcześniejszego zaszczepienia antagonisty niż patogena, nawet przy p = 0,01. Mechanizm ograniczenia wzrostu obejmował mykopasożytnictwo oraz konkurencję o miejsce i składniki pokarmowe. Inhibicja wzrostu patogena była różna pomiędzy (p > 0.0001) i w ramach (p > 0.026) zastosowanych metod. T. harzianum szczep 1 lepiej ograniczał wzrost patogena niż dwa pozostałe szczepy wtedy, gdy był zaszczepiony wcześniej niż patogen, podczas gdy T. harzianum szczep 3 okazał się lepszy, gdy patogena i antagonistę zaszczepiono jednocześnie (p = 0,005). Wobec powyższego różne szczepy T. harzianum mogą być wykorzystywane jako obiecujący antagoniści grzyba F. verticillioides.

Słowa kluczowe

PL

antagonist; growth; suppression; Fusarium verticillioides; Trichoderma harzianum maize; Zea mays; plant part; rhizosphere; pathogen

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2009
• Tom: 49
• Numer: 4
• Strony: 452-459
• Opis fizyczny: p.452-459,fig.,ref.

Twórcy

autor

Sobowale A A

• Olabisi Onabanjo University, P.M.B. 2002, Ago-Iwoye, Ogun State, Nigeria

autor

Odebode A.C.

autor

Cardwell K.F.

autor

Bandyopadhyay R.

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