Application of PCR technique in diagnosis of small ruminant lentivirus infection in sheep and goats

• Autorzy: Gil A. , Rola M. , Kuzmak J.
• Języki publikacji: EN

Abstrakty

EN

Detection of small ruminant lentiviruses (SRLVs) in sheep and goats usually relies on serological testing. In this study, we evaluated semi-nested PCR and nested PCR techniques applied as a diagnostic tool for detection of maedi-visna virus (MVV) and caprine arthritis-encephalitis virus (CAEV) in naturally infected sheep and goats, respectively. The examination of 193 ovine and 85 caprine serum samples by the ELISA revealed the presence of specific antibodies in 133 (69%) and 18 (21.2%) animals, respectively. Presence of proviral DNA was manifested in 103 (53.4%) sheep and 12 (14.2%) goats. Despite the relatively lower sensitivity of PCR, the fact of detection of proviral DNA in 19 out of 60 ovine samples and 7 out of 67 caprine samples collected from animals previously negative by ELISA was noteworthy. In conclusion, the data demonstrated that combinations of both ELISA and PCR might afford optimal detection of SRLVs infection.

Słowa kluczowe

EN

goat; chronic inflammatory lesion; brain; sheep; small ruminant; caprine arthritis encephalitis virus; serological testing; animal disease; maedi-visna virus; retrovirus; veterinary medicine; ELISA test; polymerase chain reaction; lung; mammary gland; lentovirus

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2006
• Tom: 09
• Numer: 4
• Opis fizyczny: p.213-217,fig.,ref.

Twórcy

autor

Gil A.

• National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland

autor

Rola M.

autor

Kuzmak J.

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