Immunohistochemical characterization of choline acetyltransferase-containing nerve fibres supplying the vas deferens in the pig

• Autorzy: Kaleczyc J. , Klimczuk M. , Wasowicz K. , Majewski M. , Calka J. , Schemann M. , Lakomy M.
• Języki publikacji: EN

Abstrakty

EN

Single-, double- and triple-labelling immunohistochemistry were applied to study the distribution and neurochemical properties of cholinergic nerve fibres supplying the vas deferens in juvenile and adult pigs. Cholinergic nerves were identified using an antibody against choline acetyltransferase. Immunoblotting was applied to verify the specificity of choline acetyltransferase immunostaining. Western blotting performed on vas deferens tissue homogenates detected single immunoreactive protein with a molecular weight matching this of acetylcholine transferase (71,5 kDa). The majority of choline acetyltransferase-containing nerves innervating the organ are distributed in the lamina propria and coexpress immunoreactivities of up to 4 other biologically active substances including nitric oxide synthase (a marker of nitrergic structures) and neuropeptides, vasoactive intestinal polypeptide, neuropeptide Y and somatostatin. A comparison of data previously collected with the present results reveals that in the pig, neurochemical characteristics of choline acetyltransferase-containing pelvic neurons and nerve fibres supplying the vas deferens are very similar leading to conclusion that cholinergic innervation of the organ largely derives from pelvic ganglia.

Słowa kluczowe

EN

pig; neuropeptide; nerve fibre; immunohistochemistry; cholinergic innervation; choline acetyltransferase

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2000
• Tom: 03
• Numer: 2
• Opis fizyczny: p.111-118,fig.

Twórcy

autor

Kaleczyc J.

• University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-719 Olsztyn-Kortowo, Bl.105J, Poland

autor

Klimczuk M.

autor

Wasowicz K.

autor

Majewski M.

autor

Calka J.

autor

Schemann M.

autor

Lakomy M.