Acquisition of iron by enterococci: some properties and role of assimilating ferric iron reductases

• Autorzy: Lisiecki P , Mikucki J.
• Języki publikacji: EN

Abstrakty

EN

Ferric iron reductases activities have been occurred in 91% of investigated enterococci strains. Maximum activity occurred with coenzyme NADH as the reductant and the presence of cofactor FMN was necessary. Mg(II) ions has not stimulated reductases activity. Treatment of cells with proteolytic enzymes had not effect on iron reduction. The whole cells and cell fraction - cytoplasmic membrane and cytoplasm showed Fe(III) - reducing activity. The highest specific activity was associated with cytoplasm. The activity in cytoplasmic membrane was not related to iron concentration in the growth medium. In cytoplasm the activity was stimulated after growth in low-iron medium. Ferric iron reductases of enterococci characterized the broad substrate specificity. The iron in form of ferric ammonium citrate, lactoferrin and ferrioxamine B were the best iron sources for enterococcal ferric iron reductases.

Słowa kluczowe

EN

iron; growth medium; iron concentration; activity; ferric ammonium citrate; ferrioxamine B; ferric iron reductase; iron acquisition; Enterococcus; iron source; lactoferrin

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2006
• Tom: 55
• Numer: 4
• Opis fizyczny: p.271-277,ref.

Twórcy

autor

Lisiecki P

• Medical University, Pomorska 137, 90-235 Lodz, Poland

autor

Mikucki J.

Bibliografia

• Arceneaux J.E. and B.R. Byers. 1980. Ferrisiderophore reductase activity in Bacillus megaterium. J. Bacteriol. 141: 715-721.
• Barchini E. and R.E. Cowart. 1996. Extracellular iron reductase activity produced by Listeria monocytogenes. Arch. Microbiol. 166: 51-57.
• Clarke T.E, L.W. Tari and H.J. Vogel. 2001. Structural biology of bacterial iron uptake systems. Curr. Top. Med. Chem. 1: 7-30.
• Cowart R.E., F.L. Singleton and J.S. Hind. 1993. A comparison of bathophenanthrolinedisulfonic acid and ferrozine as chelators of iron(II) in reduction reactions. Anal. Biochem. 211: 151-155.
• Cowart R.E. 2002. Reduction of iron by extracellular iron reductases: implications for microbial iron acquisition. Arch. Biochem. Biophys. 400: 273-281.
• Coves J. and M. Fontecave. 1993. Reduction and mobilization of iron by a NAD(P)H: flavin oxidoreductase from Escherichia coli. Eur. J. Biochem. 211: 635-641.
• Dixon W.J. and F.J. Massey. 1951. Introduction to statistical analysis. McGraw-Hill Book Co., New York.
• Deneer H.G., V. Healey and I. Boychuk. 1995. Reduction of exogenous ferric iron by a surface-associated ferric reductase of Listeria spp. Microbiology 141: 1985-1992.
• Fontecave M., R. Eliasson and P. Reichard. 1987. NAD(P)H: flavin oxidoreductase of Escherichia coli. A ferric iron reductase participating in the generation of the free radical of ribonucleotide reductase. J. Biol. Chem. 262: 12325-12331.
• Gadia M.K. and M.C. Mehra. 1977. Rapid spectrophotometric analysis of total and ionic iron in the pg range. Microchemica Acta (Wien) 11: 413-418.
• Halle F. and J.M. Meyer. 1992. Ferrisiderophore reductases of Pseudomonas. Purification, properties and cellular location of the Pseudomonas aeruginosa ferripyoverdine reductase. Eur. J. Biochem. 209: 613-620.
• Homuth M., P. Valentin-Weigand, M. Rohde and G.F. Gerlach. 1998. Identification and characterization of a novel extracellular ferric reductase from Mycobacteriumparatuberculosis. Infect. Immun. 66: 710-716.
• Johnson W., L. Varner and M. Poch. 1991. Acquisition of iron by Legionella pneumophila: role of iron reductase. Infect. Immun. 59: 2376-2381.
• Le Faou A.E. and S.A. Morse. 1991. Characterization of a soluble ferric reductase from Neisseria gonorrhoeae. Biol. Met. 4: 126-131.
• Lindberg M. 1981. Genetic studies in Staphylococcus aureus using protoplast: cell fusion and transformation, p. 535-541. In: J. Jeljaszewicz (ed.), Staphylococcus and staphylococcal infection, Zentralbl. Bakteriol. Suppl. 10, G. Fischer Verlag, Stuttgard, New York.
• Lisiecki P. and J. Mikucki. 2005. Assimilatory ferric reductases in enterococci (in Polish). Med. Dośw. Mikrobiol. 57: 359-368.
• Lowry H., N.J. Rosebrough, A. Lewis Farr and R.J. Randall. 1951. Protein measurment with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
• Mazoy R. and M. Lemos. 1999. Ferric-reductase activities in Vibrio vulnificus biotype 1 and 2. FEMS Microbiol. Lett. 172: 205-211.
• Ratledge C. and L.G. Dover. 2000. Iron metabolism in pathogenic bacteria. Annu. Rev. Microbiol. 54: 881-941.
• Schröder I., E. Johnson and S. de Vries. 2003. Microbial ferric iron reductases. FEMS Microbiol. Rev. 27: 427-447.
• Silver S. and M. Walderhaug. 1992. Gene regulation of plasmid - and chromosome-determined inorganic ion transport in bacteria. Microbiol. Rev. 56: 195-228.
• Stookey L.L. 1970. Ferrozine - a new spectrophotometric reagent for iron. Anal. Biochem. 42: 779-781.
• Vartivarian S.E. and R. Cowart. 1999. Extracellular iron reductases: identification of anew class of enzymes by siderophore-producing microorganisms. Arch. Biochem. Biophys. 364: 75-82.
• Zorzi W., X.Y. Zhou, O. Dardeune, J. Lamotte, D. Raze, J. Pierre, L. Gutmann and J. Coyette. 1996. Structure of the low-affinity Penicillin-Binding Protein 5 PBP fm in wild-type and highly penicillin-resistant strains of Entero-coccus faecium. J. Bacteriol. 178: 4948-4957.