A study of antioxidant properties from Garcinia mangostana L. pericarp extract

• Autorzy: Zarena A. S. , Sankar K.U.

Warianty tytułu

PL

Ocena wlasciwosci przeciwutleniajacych ekstraktow owocow mangostanu wlasciwego - smaczeliny [Garcinia mangostana L.]

• Języki publikacji: EN

Abstrakty

EN

The present work was undertaken to assess the radical scavenging and antioxidant properties of the mangosteen (Garcinia mangostana L.) pericarp extracts obtained by various solvents and its mixtures of different polarity. The extracts were evaluated by FRAP (Ferric reducing antioxidant power), ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulfonicacid) diammonium salt], DPPH (1,1-diphenyl-2-picrylhydrazyl), reducing power and chelating ability on ferrous ion. Among the different extracts studied, ethyl acetate and acetone extract were observed to have good antioxidant activity. The FRAP values for ethyl acetate and acetone extract were 1.30 and 1.01 mM TEAC respectively at 1 mg/ml. The TEAC values for ABTS assay were 38.21 and 38.15 μM respectively at 100 μg/ml. The IC50 values to quench the DPPH free radicals of these two extracts were 30.01 μg/ml and 33.32 μg/ml, but the chelating assay was observed to be better in aqueous fractions of Acetone: water (80:20) at 12 mg/ml. From the above results it can be inferred that ethyl acetate and acetone were suitable solvent to extract the antioxidant compounds form mangosteen.

PL

W pracy podjęto próbę oznaczenia aktywności przeciwrodnikowej i prze-ciwutleniającej ekstraktów owoców mangostanu właściwego – smaczeliny (Garcinia mangostana L.) uzyskanych na drodze ekstrakcji rozpuszczalnikami o różnej polarności. Ocena obejmowała oznaczenie FRAP, ABTS+°, DPPH°, siły redukującej oraz zdolności chelatowania jonów żelaza. Spośród badanych ekstraktów największą aktywność przeciwutleniającą stwierdzono w próbach ekstrahowanych octanem etylu i acetonem. Wartości oznaczeń FRAP dla stężenia 1 mg/ml ekstraktów octanu etylu i acetonu wynosiły odpowiednio: 1,30 i 1,01 mM TEAC, natomiast dla oznaczeń ABTS wynosiły 38,21 i 38,15 mM. Stwierdzono, że wartości IC50 zmiatania rodników DPPH obu wymienionych ekstraktów wynosiły odpowiednio 30,01 i 33,32 μ/ml. Analiza wyników dotyczących zdolności chelatowania jonów żelaza wskazała na większą aktywność frakcji wodnej ekstraktów aceton – woda (80:20). Na podstawie uzyskanych wyników badań można stwierdzić, że zarówno octan etylu, jak i aceton są odpowiednimi rozpuszczalnikami wykorzystanymi do ekstrakcji związków przeciwutleniających z owocni mangostanu.

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Technologia Alimentaria
• Rocznik: 2009
• Tom: 08
• Numer: 1
• Strony: 23-34
• Opis fizyczny: p.23-34,fig.,ref.

Twórcy

autor

Zarena A. S.

• Central Food Technological Research Institute in Mysore, India

autor

Sankar K.U.

Bibliografia

• Akao Y., Nakagawa Y., Iinuma M., Nozawa Y., 2008. Anticancer effects of xanthones from pericarps of mangosteen. Int. J. Mol. Sci. 9, 355-370.
• Benzie I.F., Szeto Y.T., 1999. Total antioxidant capacity of teas by the ferric reducing/antioxidant power assay. J. Agric. Food Chem. 47, 633-636.
• Chairungsrilerd N., Furukawa K., Ohta T., Nozoe S., Ohizumi Y., 1996. Pharmacological properties of alpha-mangostin, a novel histamine H1 receptor antagonist. Eur. J. Pharm. 314, 351- -356.
• Dubois M., Gilles K.A., Hamilton J.K, Rebers P.A., Smith F., 1956. Colorimetric method for determination of sugar and related substances. Anal. Chem. 28, 350-356.
• Etherton K.P.M., Hecker K.D., Bonanome A., Coval S.M., Binkoshi A.E., Hilpert K.D., Griel A.E., Etherton T.D., 2002. Bioactive compound in foods their role in preventation of cardio-vascular disease and cancer. Am. J. Med. 113, 71-88.
• Jung H.A., Su B.N., Keller W.J., Mehta R.G., Kinghorn A.D., 2006. Antioxidant xanthones from the pericarp of Garcinia mangostana (Mangosteen). J. Agric. Food Chem. 54, 2077-2082.
• Ka¨hko¨nen M.P., Hopia A.I., Vuorela H.J., Rauha J.P., Pihlaja K., Kujala T.S. Heinonen M., 1999. Antioxidant activity of plant extracts containing phenolic compounds. J. Agric. Food Chem. 47, 3954-3962.
• Ka¨hko¨nen M.P., Hopia A.I., Heinonen M., 2001. Berry phenolics and their antioxidant activity. J. Agric. Food Chem. 49, 4076-4082.
• Kosema N., Hanb H.Y., Moongkarndia P., 2007. Antioxidant and cytoprotective activities of methanolic extract from Garcinia mangostana hulls. Sci. Asia. 33, 283-292.
• Kujala T.S., Loponen J.M., Klika K.D., Pihlaja K., 2000. Phenolics and betacyanins in red beet-root (Beta vulgaris) root: distribution and effect of cold storage on the content of total phenolics and three individual compounds. J. Agric. Food Chem. 48, 5338-5342
• Lafka T.I., Sinanoglou V., Lazos E.S., 2007. On the extraction and antioxidant activity of phenolic compounds .from winery wastes. Food Chem. 104, 1206-1214.
• Mahabusarakam W., Proudfoot J., Taylor W., 2000. Inhibition of lipoprotein oxidation by prenylated xanthones derived from mangostin. Free Radical Res. 33, 643-659.
• Marinova E.M., Yanishlieva, N.V., 1997. Antioxidative activity of extracts from selected species of the family lamiaceae in sunflower oil. Food Chem. 5, 245-248.
• Maisuthisakul P., Suttajit M., Pongsawatmait R., 2007. Assessment of phenolic content and free radical scavenging capacity of some Thai indigenous plants. Food Chem. 100, 1409-1418.
• Moongkarndi P., Kosem N., Kaslungka S., Luanratana O., Pongpan N., Neungton N., 2004. Antiproliferation, antioxidation and induction of apoptosis by Garcinia mangostana (mangosteen) on SKBR3 human breast cancer cell line. J. Ethnopharmacol. 90, 161-166.
• Nakatani K., Nakahata N., Arakaw, T., Yasuda H., Ohizumi Y., 2002. Inhibition of cyclooxy-genase and prostaglandin E2 synthesis by gamma-mangostin, a xanthone derivative in man-gosteen, in C6 rat glioma cells. Biochem. Pharmacol. 63, 73-79.
• Official Analytical Method of the American spice trade Association. 1985. Englewood Clliffes, New Jersey.
• Re R.E., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol. Med. 26, 1231-1237.
• Rice-Evans C.A., Miller N.J., Bolwell P.G., Bramley P.M., Pridham J.B., 1995. The relative antioxidant activities of plant derived polyphenolic flavonoids. Free Radical Res. 22, 375-383.
• Sherwin E.R., 1978. Oxidation and antioxidants in fat and oil processing. J. Am. Oil Chem. Soc. 55, 809-814.
• Suksamrarn S., Suwannapoch N., Phakhodee W., Thanuhiranlert J., Ratananukul P., Chimnoi N., Suksamrarn A., 2003. Antimycobacterial activity of prenylated xanthones from the fruits of Garcinia mangostana. Chem. Pharm. Bull. (Tokyo) 51, 857-859.
• Suter M., Richter C., 2000. Anti and prooxidative properties of PADMA 28, a Tibetan herbal formulation. Redox Rep. 5, 17-22.
• Tachakittirungrod S., Okonogi S., Chowwanapoonpohn S., 2007. Study on antioxidant activity of certain plants in Thailand: Mechanism of antioxidant action of guava leaf extract. Food Chem. 103, 381-388.
• Wang L., Yen J.H., Ling H.L., Wu M.J., 2003. Antioxidant effect of methanol extracts from lotus plumaged and Blossom (Velum nucefera gertn). J. Food Drug Anal. 11, 60-66.
• Yen G.C, Duh P.D., Chuang D.Y., 2000. Antioxidant activity of anthraquinones and anthrone. Food Chem. 70, 437-441.
• Yu L., Zhao M., Yang B., Zhao Q., Jiang., Y. 2007. Phenolics from hull of Garcinia mangostana fruit and their antioxidant activities. Food Chem. 104, 176-18.