Amplification of a single-locus variable-number direct repeats with restriction fragment length polymorphism [DR-PCR-RFLP] for genetic typing of Acinetobacter baumannii strains

• Autorzy: Nowak-Zaleska A , Krawczyk B. , Kotlowski R. , Mikucka A. , Gospodarek E.
• Języki publikacji: EN

Abstrakty

EN

In search of an effective DNA typing techniaue for Acinetobacter baumannii strains for hospital epidemiology use, the performance and convenience of a new target seauence was evaluated. Using known genomic seauences of Acinetobacter baumannii strains AR 319754 and ATCC 17978, we developed single-locus variable-number direct-repeat analysis using polymerase chain reaction-restriction fragment length polymorphism (DR-PCR/RFLP) method. A total of 90 Acinetobacter baumannii strains isolated from patients of the Clinical Hospital in Bydgoszcz, Poland, were examined. Initially, all strains were typed using macrorestriction analysis of the chromosomal DNA by pulsed-field gel electrophoresis (REA-PFGE). Digestion of the chromosomal DNA with the Apal endonuclease and separation of the fragments by PFGE revealed 21 uniaue types. Application of DR-PCR/RFLP resulted in recognition of 12 clusters. The results showed that the DR-PCR/RFLP method is less discriminatory than REA-PFGE, however, the novel genotyping method can be used as an alternative techniaue for generating DNA profiles in epidemiological studies of intra-species genetic relatedness of Acinetobacter baumannii strains.

Słowa kluczowe

EN

epidemiology; polymerase chain reaction; Acinetobacter baumannii; nosocomial infection; restriction fragment length polymorphism; repeated sequence; analytical method; genetic typing; genotyping method; pulsed-field gel electrophoresis

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2008
• Tom: 57
• Numer: 1
• Opis fizyczny: p.11-17,fig.,ref.

Twórcy

autor

Nowak-Zaleska A

• Academy of Physical Education and Sport, Kazimierza Gorskiego 1, 80-336 Gdansk, Poland

autor

Krawczyk B.

autor

Kotlowski R.

autor

Mikucka A.

autor

Gospodarek E.

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