Fenotyp i genotyp szczepow z rodzaju Clostridium wytwarzajacych neurotoksyny botulinowe

• Autorzy: Augustynowicz E. , Gzyl A. , Gniadek G. , Slusarczyk J.
• Języki publikacji: PL

Abstrakty

PL

W pracy podjęto określenie fenotypu i genotypu szczepów C. botulinum. Poszukiwano również genów kodiyących BoNT typu А, В oraz E wśród szczepów rodzaju Clostridium. Uzyskane wyniki wskazują na powszechność występowania genu kodującego BoNT/E wśród szczepów rodząju Clostridium. Analiza fenotypu i genotypu szczepów C. botulinum wykazała występowanie szczepów fenotypu В o genotypie be jak również szczepów fenotypu A o genotypie ae.

EN

Neurotoxins produced by strains of Clostridium sp. are belonging to the most toxic biological substances. In the study phenotypes and genotypes of C. botulinum strains in animal studies in Vivo and on the DNA level were evaluated, respectively. Additionally, the presence of genes encoding BoNT toxins of A, B, and E types among strains of Clostridium sp. were identified. In case of C. botulinum DNA was isolated from vegetative bacterial cells and from spores. Two different genes encoding two different neurotoxins harboured by three strains of Ae biotype/ae genotype, and by two strains of B biotype/be genotype were detected. Additionally, above E type C. botulinum strains, the presence of gene encoding E type neurotoxin, was found in genome of two C. baratii, two C. butyricum, and C. bifidobacterium, and C. oedematicum strains. C. bifidobacterium and C. oedematicum strains positive for presence of gene encoding E type neurotoxin, were found negative for E neurotoxin production in vivo in TN test. The study indicates that genes encoding BoNT/E neurotoxins are very common among Clostridium species. Phenotype and genotype analysies indicated co-presence of В phenotype together with be genotype and A phenotype together with ae genotype among C. botulinum strains.

Słowa kluczowe

PL

neurotoksyny botulinowe; genotyp; szczepy bakteryjne; neurotoksyny; fenotyp; bakterie; Clostridium; toksycznosc

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2003
• Tom: 55
• Numer: 3
• Strony: 244-252
• Opis fizyczny: s.244-252,tab.,bibliogr.

Twórcy

autor

Augustynowicz E.

• Panstwowy Zaklad Higieny, Warszawa

autor

Gzyl A.

autor

Gniadek G.

autor

Slusarczyk J.

Bibliografia

• 1. Aranda E, Rodriguez MM, Asensio MA i inni. Detection of Clostridium botulinum types А, В, E, and F in foods by PCR and DNA probes. Lett Appl Microbiol 1997; 25: 186-90.
• 2. Bhandari M, Campbell KD, Collins MD i inni. Molecular characterization of the clusters of genes encoding the botulinum neurotoxin complex in Clostridium botulinum (Clostridium argentinense) type G and nonproteolytic Clostridium botulinum type B. Curr Microbiol 1997; 35: 207-14.
• 3. Cordoba JJ, Collins ML, East AK. Studies on the genes encoding botulinum neurotoxin type A of Clostridium botulinum from a variety of sources. Syst Appl Microbiol 1995; 18: 13-22.
• 4. Fach P, Gilbert M, Griffais R i inni. Investigation of animal botulism outbreaks by PCR and standard methods. FEMS Immunol Med Microbiol 1996; 13: 279-85.
• 5. Franciosa G, Fenicia L, Caldiani C i inni. PCR for detection of Clostridium botulinum type C in avian and environmental samples. J Clin Microbiol 1996; 34: 882-85.
• 6. Franciosa G, Ferreira JL, Hatheway ChL. Detection of type A, B, and E botulism neurotoxin genes in Clostridium botulinum and other Clostridium species by PCR: evidence of unexpressed type В toxin genes in type A toxigenic organisms. J Clin Microbiol 1994; 32: 1911-17.
• 7. Franciosa G, Hatheway C, Aureli P. The detection of a deletion in the type В neurotoxin gene of Clostridium botulinum A (B) strains by a two-step PCR. Lett Apel Microbiol 1998; 26: 442-6.
• 8. Hall JD, McCroskey LM, Pincomb BJ i inni. Isolation of an organism resembling Clostridium baratii which produces type F botulinal toxin from an infant with botulism. J Clin Microbiol 1985; 21: 654-5.
• 9. Hatheway CH, McCroskey LM, Lombard GL i inni. Atypical toxin variant of Clostridium botulinum type В associated with infant botulism. J Clin Microbiol 1981;14: 607-11.
• 10. Henderson I, Davis T, Elmore MI inni. The genetic basis of toxin production in Clostridium botulinum and Clostridium tetani. In: Rood JI, McClane BA, Songer JG, Titball RW, editors. The Clostridia. Molecular Biology and Pathogenesis Red. J.I. Rood, B.A. McClane, J.G. Songer, R.W. Titball, Academic Press, San Diego, London, Boston, New York, Sydney, Tokyo, Toronto; 1997.p. 261-94.
• 11. McCroskey LM, Hatheway ChL, Woodruff BA i inni. Type F botulism due to neurotoxigenic Clostridium baratii from an unknown source in an adult. J Clin Microbiol 1991; 29: 2618-20.
• 12. Minton NP. Molecular genetics of Clostridial neurotoxins. Curr Top Microbiol 1995; 195: 161-94.
• 13. Rymkiewicz D, Sawicki J. Drobnoustroje z rodzaju Clostridium. Biologia i różnicowanie. Wydawnictwo Państwowego Zakładu Higieny 1988.
• 14. Suen JC, Hatheway CL, Steigerwalt AG i inni. Clostridium argentinense sp. nov.: a genetically homogenous group compoused of all strains of Clostridium botulinum toxin type G and some nontoxigenic strains previously identified as Clostridium subterminale or Clostridium hastiforme. Int J Syst Bacteriol 1988; 38: 375-81.
• 15. Szabo EA, Pemberton JM, Desmarchelier PM. Detection of the genes encoding botulinum neurotoxin types A to E by the polymerase chain reaction. Appl Environ Microbiol 1993; 59: 3011-20.
• 16. Szabo EA, Pemberton JM, Gibson AM i inni. Application of PCR to a clinical and environmental investigation of a case of equine botulism. J Clin Microbiol 1994; 32:1986-91.
• 17. Takeshi K, Fujinaga Y, Inoue K i inni. Simple method for detection of Clostridium botulinum type A to F neurotoxin genes by PCR. Microbiol Immunol 1996; 40: 5-11.