Inhibition of plasminogen activator inhibitor release in endothelial cell cultures by antisense oligodeoxyribonucleotides with a 5' -end lipophilic modification

• Autorzy: Kobylanska A. , Pluskota E. , Swiatkowska M. , Wojcik M. , Cierniewska-Cieslak A. , Krakowiak A. , Boczkowska M. , Pawlowska Z. , Okruszek A. , Koziolkiewicz M. , Cierniewski C.S. , Stec W.J.
• Języki publikacji: EN

Abstrakty

EN

A series of conjugates containing residues of lipophilic alcohols covalently bound to 5'-end of oligodeoxyribonucleotides targeted against human plasminogen activator inhibitor (PAI-1) mRNA was synthesized via the oxathiaphospholane approach. The highest anti-PAI-1 activity in EA.hy 926 endothelial cell cultures was found for conjugates containing menthyl or heptadecanyl groups linked with an oligonucleotide complementary to a segment of human PAI-1 mRNA. The phosphodiester antisense oligonucleotides, which otherwise exhibit only limited anti-PAI-1 activity, were found to be more active than phosphorothioate oligonucleotides when conjugated to lipophilic alcohol residues. For menthyl conjugates an evidence of antisense mechanism of inhibition was found.

Słowa kluczowe

EN

oligodeoxyribonucleotide; plasminogen activator inhibitor; lipophilic alcohol; human plasminogen; oligonucleotide conjugate; antisense inhibition

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 3
• Opis fizyczny: p.679-691,fig.

Twórcy

autor

Kobylanska A.

• Polish Academy of Sciences, H.Sienkiewicza 112, 90-363 Lodz, Poland

autor

Pluskota E.

autor

Swiatkowska M.

autor

Wojcik M.

autor

Cierniewska-Cieslak A.

autor

Krakowiak A.

autor

Boczkowska M.

autor

Pawlowska Z.

autor

Okruszek A.

autor

Koziolkiewicz M.

autor

Cierniewski C.S.

autor

Stec W.J.

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