Application of PCR for the detection of adenovirus type 1 [CAV-1] in internal organs of dogs

• Autorzy: Mizak B , Rzezutka A.
• Języki publikacji: EN

Abstrakty

EN

The aim of presented preliminary studies was the application of PCR for rapid and accurate diagnosis of CAV-1 infection in dogs. The 301 bp PCR product of CAV-1 was demonstrated in two lung and liver homogenates collected post-mortem from two dogs. The comparable studies of cytopathic effect evaluated at the 10th passage of homogenates in MDCK cells and PCR revealed 100% accordance of the results obtained by both tests. The simple and fast isolation of viral DNA with the use of Chelex was used in the presented studies. This modification of PCR procedure could also be used in field veterinary laboratories as a valuable method for detection of CAV-1 infection in dogs.

Słowa kluczowe

EN

Adenoviridae; disease; adenovirus type 1; infection; canine adenovirus; internal organ; dog; laboratory diagnosis; Rubarth's disease; polymerase chain reaction; adenovirosis

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1998
• Tom: 42
• Numer: 2
• Opis fizyczny: p.119-123,fig.

Twórcy

autor

Mizak B

• National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Rzezutka A.

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