In vitro regeneration of Punica granatum L. plants from different juvenile explants

• Autorzy: Deepika R , Kanwar K.

Warianty tytułu

PL

Regeneracja in vitro roslin Punica granatum L. z roznych eksplantatow uzyskanych z mlodych siewek

• Języki publikacji: EN

Abstrakty

EN

Reliable and reproducible protocols to get healthy and well formed plants from juvenile explants of the pomegranate (Punica granatum L.) cv. 'Kandhari Kabuli' have been developed. Calli were initiated from cotyledon, hypocotyl, leaf and internode sections excised from 30 days old in vitro germinated seedlings. The best media for callus induction from cotyledon, hypocotyl, internode and leaf explants were MS medium supplemented with 13.0 ^M NAA and 13.5 ^M BA, 13.0 ^M NAA and 18.0 ^M BA, 5.0 ^M IBA and 9.0 ^M BA, 8.0 ^M NAA and 9.0 ^M kinetin, respectively. The highest percentage of callus was obtained from cotyledon explants (85.50) followed by hypocotyl (79.67), internode (79.47) and leaf (75.48) explants. The calli thus obtained showed differentiation on MS medium supplemented with 9.0 ^M BA and 2.5 ^M NAA. Cotyledon derived callus showed the highest regeneration rate (81.97%, with mean number of 16.47 shoots per explant) followed by hypocotyl, internode and leaf derived calli. In vitro rooting was best on half strength MS medium containing 500 mg l-1 of activated charcoal. The plantlets with well formed root systems were transferred to plastic cups containing cocopeat followed by transfer to earthen pots containing soil and sand (1:1).

PL

Przedstawiono wiarygodny i odtwarzalny przebieg doświadczenia mającego na celu otrzymanie zdrowych i kształtnych roślin z eksplantatów uzyskanych z młodych siewek granatowca Punica granatum L. 'Kandhari Kabuli'. Kalusy otrzymano z wycinków liścienia, hypokotyla, liścia i łodygi z 30-dniowych sadzonek doprowadzonych do kiełkowania metodą in vitro. Najlepszą pożywką dla indukcji embriologicznej kalusa z eksplantatów pochodzących z liścienia, hypokotyla, łodygi i liścia były pożywki MS uzupełnione odpowiednio 13,0 ^M NAA i 13,5 ^M BA, 13,0 ^M NAA i 18,0 ^M BA, 5,0 ^M IBA i 9,0 ^M BA, 8,0 ^M NAA i 9,0 ^M kinetyny. Największy procent kalusów otrzymano z eksplantatów uzyskanych z liścienia (85,50), następnie z hypokotyla (79,67), łodygi (79,47) i liścia (75,48). Otrzymane w ten sposób kalusy wykazafy zróżnicowanie na pożywce MS uzupełnionej 9,0 ^M BA i 2,5 ^M NAA. Kalus uzyskany z liścienia wykazał najwyższy wskaźnik regeneracji (81,97% ze średnią liczbą 61,47 pędów na eksplantant), następnie uzyskany z hypocotyla, łodygi i liścia. Ukorzenianie metodą in vitro dało najlepsze wyniki na zmniejszonej o połowę pożywce MS i zawierającej 500 mg l-1 węgla aktywnego. Roślinki z dobrze ukształtowanym systemem korzeniowym byty przeniesione z plastikowych pojemników zawieraj ących włókna kokosowe do glinianych doniczek zawierających ziemię i piasek (1:1).

Słowa kluczowe

EN

in vitro; regeneration; Punica granatum; pomegranate; callus culture; juvenile explant; tropical region; subtropical region

• Wydawca: -
• Czasopismo: Journal of Fruit and Ornamental Plant Research
• Rocznik: 2010
• Tom: 18
• Numer: 1
• Strony: 5-22
• Opis fizyczny: p.5-22,fig.,ref.

Twórcy

autor

Deepika R

• University of Pretoria, Pretoria 0002, South Africa

autor

Kanwar K.

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