Lymphocyte DNA damage in rats challenged with a single bout of strenuous exercise

• Autorzy: Wierzba T.H. , Olek R.A. , Fedeli D. , Falcioni G.
• Języki publikacji: EN

Abstrakty

EN

Exercise induces extensive generation of reactive oxygen species, which are responsible for tissue damage: enzymes inactivation, lipid peroxidation and single strand breaks in DNA. Defense system against free radicals is consisting of enzymes such as superoxide dismutase, catalase, glutathione peroxidase, and numerous non-enzymatic antioxidants. The study was performed to evaluate the effect of a single bout of submaximal running exercise, on the lymphocyte DNA strand breaks and also to test how supplementation with tempol – a membrane-permeable SOD-mimetic (0.2 mmol/kg/day) influences the eventually evoked damage. Male, Wistar rats were challenged with graded 50 min. running on treadmill at intensity up to 75-85% of predicted O2 max. The DNA strand breaks in individual lymphocytes were determined by using a gel electrophoretic technique – “comet” assay. We found substantial lymphocyte DNA damage 60 min. after the exercise. Tempol failed to prevent from oxidative damage in rats challenged with exercise. Moreover tempol by itself induced higher DNA damage than the exercise bout.

Słowa kluczowe

EN

lymphocyte; DNA damage; rat; strenuous exercise; reactive oxygen species; tissue damage; enzyme inactivation; lipid peroxidation; single stand break; free radical; superoxide dismutase; catalase; glutathione peroxidase; enzymatic antioxidant; comet assay

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology. Supplement
• Rocznik: 2006
• Tom: 57
• Numer: 10
• Strony: 115-131
• Opis fizyczny: p.115-131,fig.,ref.

Twórcy

autor

Wierzba T.H.

• Medical University of Gdansk, 1 Debinki Str., 80-211 Gdansk, Poland

autor

Olek R.A.

autor

Fedeli D.

autor

Falcioni G.