Induction of ornithine decarboxylase in normal and protein kinase C - depleted human colon carcinoma cells

• Autorzy: Ostrowski J , Szczepiorkowski Z. , Trzeciak L. , Rochowska M. , Skurzak H. , Butruk E.
• Języki publikacji: EN

Abstrakty

EN

To examine the role of protein kinase С (PKC) in induction of human colon adenocarcinoma cell line, DETA/W, by polypeptide growth- promoting factors, ornithine decarboxylase activity (ODC) and DNA synthesis were determined in cells depleted of PKC. PKC depletion was achieved by prolonged cultivation (more than 30 passages) with 10⁻⁶ M phorbol 12-myristate 13-acelate. Lack of PKC in studied cells was proved by measurements of PKC activity and immunoreactivity. Although ODC activities and DNA syntheses in PKC-depleted cells were decreased by about 40-50% compared to normal DETA/W cells, the percentage increase of these mitogen-responsive reactions was quantitatively similar in both cell sublines. These results raise the possibility that not all of the biological responses to growth factors are connected with the activation of calcium-dependent PKC.

Słowa kluczowe

EN

carcinoma cell; adenocarcinoma cell; doexyribonucleic acid; ornithine decarboxylase; DNA synthesis; human colon; protein kinase C

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 1992
• Tom: 43
• Numer: 4
• Opis fizyczny: p.373-382,fig.

Twórcy

autor

Ostrowski J

• Medical Center of Postgraduate Education, 01-813 Warszawa, Marymoncka 99, Poland

autor

Szczepiorkowski Z.

autor

Trzeciak L.

autor

Rochowska M.

autor

Skurzak H.

autor

Butruk E.

Bibliografia

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