Changes in free fatty acid composition in Chlorella vulgaris at various stages of the life cycle

• Autorzy: Lodowska J. , Tam I. , Mazurek U. , Kurkiewicz S. , Pytel A. , Wilczok A. , Wilczok T.
• Języki publikacji: EN

Abstrakty

EN

The aim of the presented work was to determine and calculate the free fatty acid (FFA) total concentration at various stages of Chlorella vulgaris cells cultivated synchronously. The FFA were isolated from cells harvested at given life cycle stages by the use of the SPE method, methylated to methyl esters and analyzed qualitatively and semiquantitavely by GC/MS. The total FFA concentration decreased during the time course of cultivation, the amount of unsaturated FFA decreased, and the concentration of saturated FFA increased. The highest concentration was shown for C16:0. Statistical analysis showed that FFA at various stages of the Chlorella vulgaris life cycle may be grouped in classes which demonstrate similar changes in concentrations when observed during the whole life cycle.

Słowa kluczowe

EN

statistical analysis; synchronic cultivation; concentration; life cycle; methyl ester; fatty acid; Chlorella vulgaris; free fatty acid

• Wydawca: -
• Czasopismo: Acta Biologica Cracoviensia. Series Botanica
• Rocznik: 1997
• Tom: 39
• Opis fizyczny: p.91-97,fig.

Twórcy

autor

Lodowska J.

• Silesian Medical Academy, ul.Jagiellonska 4, 41-200 Sosnowiec, Poland

autor

Tam I.

autor

Mazurek U.

autor

Kurkiewicz S.

autor

Pytel A.

autor

Wilczok A.

autor

Wilczok T.

Bibliografia

• Ahlgren, G., I. Gustafsson, and M. Boberg. 1992. Fatty acid content and chemical composition of freshwater microalgae. Journal of Phycology 28: 37-50.
• Tan, C. K., and M. R. Johns. 1991. Fatty acid production by heterotrophic Chlorella saccharophila. Hydrobiologica 215: 13-19.
• Cranwell, P. A., and G. H. M. Bickley. 1990. Hydrocarbons, sterols, esters and fatty acids in six freshwater chlorophytes. Phytochemistry 29: 145-151.
• Guckert, J. B., and K. E. Cooksey. 1990. Triglyceride accumulation and fatty acid profile changes in Chlorella (Chlorophyta) during high pH-induced cell cycle inhibition. Journal of Phycology 26: 72-79.
• Kinsella, J. E., S. Broughton, and J. W. Whelan. 1990. Dietary unsaturated fatty acids: interactions and possible needs in relation to eicosanoid synthesis. Journal of Nutritional Biochemistry 1: 123-141.
• Mazurek, U. 1993. Changes of C18 fatty acids content in phospholipids of synchronously cultured Chlorella vulgaris. Acta Biochemica Polonica 40: 120-122.
• Mayzand, P., H. Claustre, and P. Augier. 1990. Effect of variable nutrient supply on fatty acid composition of phytoplankton grown in an enclosed experimental ecosystem. Marine Ecology Progress Series 60: 123-140.
• Nichols, B. W. 1965. Light induced changes in the lipids of Chlorella vulgaris. Biochimica et Biophysica Acta 106: 274-279.
• Otsuka, H. 1961. Changes of lipid and carbohydrate contents in Chlorella cells during the sulfur starvation, as studied by the technique of synchronous culture. Journal of General and Applied Microbiology 7: 72-77.
• Otsuka, H. 1963. Contents of sterols in Chlorella cells at different developmental stages. Plant and Cell Physiology 4: 293-297.
• Rezanka, T., P. Mares, P. Husek, and M. Podojil. 1986. Gas chromatography - mass spectrometry and desorption chemical jonization mass spectrometry of triacylglycerols from the green alga Chlorella kessleri. Journal of Chromatography 355: 265-271.
• Sargent, J. R., R. J. Parkes, I. Mueller-Harvey, and R. J. Henderson. 1987. Lipid biomarkers in marine ecology. In: M. A. Sleigh [ed.], Microbes in the sea, 119-138. Ellis Horwood Ltd., Chichester.
• Suen, Y., J. Hubbard, G. Holzer, and T. G. Tornabene 1987. Total lipid production of the green alga Nannochloropsis sp. QH under different nitrogen regimes. Journal of Phycology 23: 289-296.
• Thomson, P. A., P. J. Harrison, and J. N. C. Whyte. 1990. The influence of irradiance on the fatty acid composition of phytoplankton. Journal of Phycology 26: 278-288.
• Wilczok, A., and U. Mazurek. 1987. The role of water in size and shape characteristics of newly formed aplanospores of Chlorella vulgaris. Studia Biophysica 122: 237-248.