Heterogeneity of galF and gnd of the cps region for capsule synthesis in clinical isolates of Klebsiella pneumoniae

• Autorzy: Gierczynski R
• Języki publikacji: EN

Abstrakty

EN

The capsular polysaccharide (CPS) plays important role in Klebsiella spp pathogenesis. Capsular types Kl and K.2 of Klebsiella pneumoniae are considered most virulent for humans. The capsule biosynthesis region flanking genes galF and gnd from clinical isolates and reference strains of K. pneumoniae were screened for polymorphism. Nucleotide sequence analysis of galF and gnd revealed a high heterogeneity. However, deduced amino acid sequences demonstrated that the majority of mutations were silent implying GalF and Gnd are strongly conserved. This may suggest importance of these loci in the CPS biosynthesis and may argue for their potential usefulness in Klebsiella genotyping.

Słowa kluczowe

EN

pathogenesis; isolate; clinical isolate; capsule synthesis; pathogen; capsular polysaccharide region; Klebsiella pneumoniae; heterogeneity

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2007
• Tom: 56
• Numer: 2
• Opis fizyczny: p.83-88,fig.,ref.

Twórcy

autor

Gierczynski R

• National Institute of Hygiene, Chocimska 24, Warsaw, Poland

Bibliografia

• Altschul S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller and D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402.
• Arakawa Y., R. Wacharotayankun, T. Nagatsuka, H. Ito, N. Kato and M. Ohta. 1995. Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid. Infect. Immun. 177: 1788-1796.
• Astorza B., G. Cortes, C. Crespi, C. Saus, J.M. Rojo and S. Alberti. 2004. C3 promotes clearance of Klebsiella pneumoniae by A549 epithelial cells. Infect. Immun. 72: 1767-1774.
• Brisse S., S. Issenhuth-Jeanjean and P.A.D. Grimont. 2004. Molecular serotyping of Klebsiella species isolates by restriction of the amplified capsular antigen gene cluster. J. Clin. Microbiol. 42: 3388-3398.
• Fung C.-P., F.-Y. Chang, S.-C. Lee, B.-S. Hu, B. I.-T. Kuo, C.-Y. Liu, M. Ho and L.K. Siu. 2002. A global emerging disease of Klebsiella pneumoniae liver abscess: is serotype K1 an important factor for complicated endophthalmitis? Gut 50: 420-424.
• Gierczyński R., S. Kałużewski, A. Rakin, M. Jagielski, A. Zasada, A. Jakubczak, B. Borkowska-Opacka and W. Rastawicki. 2004. Intriguing diversity of Bacillus anthracis in eastern Poland - the molecular echoes of the past outbreaks. FEMS Microbiol. Lett. 239: 235-240.
• Gierczyński R., S. Kałużewski, A.A. Zasada, W. Rastawicki and M. Jagielski. 2005a. Occurrence of selected loci of the cps region for capsule K1 or K2 synthesis in epidemic strains of Klebsiella pneumoniae isolated from infants in Poland, (in Polish) Med. Dośw. Mikrobiol. 57: 51-63.
• Gierczyński R., S. Kałużewski, A.A. Zasada, W. Rastawicki and M. Jagielski. 2005b. Single strand conformation polymorphism (SSCP) of selected loci of the cps region for capsule synthesis in epidemic and occasionaly isolated strains of Klebsiella pneumoniae in Poland (in Polish). Med. Dośw. Mikrobiol. 57: 153-161.
• Kałużewski S. 1965. Biochemical properties of Klebsiella pneumoniae applied to strain typing. Exp. Med. Microbiol. 17: 1-8.
• Kałużewski S. 1968. Some partial agents of unencapsulated variants of group 02 Klebsiella. I. Characteristics of strains and antigen O preparations. Exp. Med. Microbiol. 20:16-32.
• Kumar S., K. Tamura, LB. Jakobsen and M. Nei. 2001. MEGA2: Molecular Evolutionary Genetics Analysis software. Bio-informatics 17: 1244-1245.
• Lai, Y.-Ch., H.-L. Peng and H.-Y. Chang. 2003. RmpA2, an activator of capsule biosynthesis in Klebsiella pneumoniae CG43, regulates K2 cps gene expression at the transcriptional level. J. Bacteriol. 185: 788-800.
• Nelson K. and R.K. Selander. 1994. Intergeneric transfer and recombination of the 6-phosphogluconate dehydrogenase gene (gnd) in enteric bacteria. Proc. Natl. Acad. Sci. USA 91: 10227-10231.
• Podschun R. and U. Ulmann. 1998. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin. Microbiol. Rev. 11: 589-603.
• Rahn A., J. Drummelsmith and C. Whitfield. 1999. Conserved organisation of the cps gene clusters for expression of Escherichia coli group 1K antigens: relationship to colanic acid biosynthesis locus and the cps genes from Klebsiella pneumoniae. J. Bacteriol. 181: 2307-2313.