Inhibition of GA3-induced antheridiogenesis in Anemia phyllitidis by peptidic extracts from male sex organs of Chara

• Autorzy: Kazmierczak A , Maszewski J.
• Języki publikacji: EN

Abstrakty

EN

Low molecular weight peptidic component extracted from maturing male sex organs of Chara tomentosa, capable of inducing increased condensation of chromosomes and profound changes in the cell cycle progression, was applied to gametophytes of Anemia phyllitidis. Morphogenetic effects were studied with regard to cell divisions and GA₃-induced antheridiogenesis. As compared with both the GA⁻₃ and GA⁺₃ control samples, the extract-treated prothallia exhibited considerably lowered number of cells and altered morphology. Antheridial differentiation in prothallia of A. phyllitidis was severely inhibited when peptidic extract was added to medium containing GA₃. Considering endocytotic uptake, evidenced in root meristems, and those effects which have been observed in plant and human cells, the activity of extracts obtained from male sex organs of Chara may be interpreted as inhibitory influence acting via repression or modification of the genetic device of the cells rather, than a direct consequence of the retardation of cell division cycles.

Słowa kluczowe

EN

Anemia phyllitidis; Chara tomentosa; Chara; male; antheridium; sex organ; peptidic extract; spermatogenesis

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 1997
• Tom: 19
• Numer: 3
• Opis fizyczny: p.269-276,fig.

Twórcy

autor

Kazmierczak A

• University of Lodz, 90-231 Lodz, Pilarskiego 14, Poland

autor

Maszewski J.

Bibliografia

• Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the protein-dye binding. Anal. Biochem. 72: 248–254.
• Corey E.J., Myers A.G., Takahashi N., Yamane H., Schraudolf H. 1986. Constitution of antheridium-inducing factor of Anemia phyllitidis. Tetrahedron Letters 27: 5083–5084.
• Fechner A., Schraudolf H. 1986. Aphidicolin inhibition of DNA synthesis and germination in spores of Anemia phyllitidis L. Sw. Plant Physiol. 81: 714–716.
• Godlewski M., Olszewska M.J. 1973. Comparison of the duration of the cell cycle in successive generations of synchronously dividing antheridial filaments of Chara vulgaris L. as measured with 3H-thymidine. Acta Soc. Bot. Pol. 42: 121–131.
• Huttly A.K., Phillips A.L. 1995. Gibberellin-regulated plant genes. Physiol. Plant 95: 310–317.
• Kaźmierczak A., Maszewski J. 1995. Inhibition of root elongation in antheridial chromatin condensation factor-treated seedlings of Allium cepa and Melandrium noctiflorum. Biol. Bull. Poznań, Supplement 32: 14.
• Kwiatkowska M., Maszewski J. 1979. Changes in the activity of RNA polymerase detected in situ and the intesity of 3H-uridine incorporation into the nucleolus and the nucleus of interphase cells in antheridial filaments of Chara vulgaris. Folia Histochem. Cytochem. 17: 275–286.
• Kwiatkowska M., Maszewski J. 1985. Nucleolar size and the activity of rRNA transport in the course of morphogenetic reduction of cell dimensions. Folia Histochem. Cytobiol. 23: 11–19.
• Maszewski J. 1981. Synthesis of nucleic acids and proteins after dark-induced blocking of mitotic divisions in antheridial filaments of Chara vulgaris L. Folia Histochem. Cytochem. 19: 55–62.
• Maszewski J. 1994. Nucleolar patterns in antheridial filaments of mono- and dioecious Chara spp. (Characeae) with different DNA C-values. Folia Histochem. Cytobiol. 32: 51–57.
• Maszewski J. 1995. Chromatin condensation factor controls proliferation → spermiogenesis transition of antheridial filaments in Chara. The Wilhelm Bernhard Workshop on the Cell Nucleus, Spa, Belgium. Abstract, pp 39.
• Miller J.H., 1968. Fern gametophytes as experimental material. The Bot. Rev. 34: 361–440.
• Mohr H. 1956. Die Abhängigkeit des Protonemawachstums und der Protonemapolarität bei Farnen vom Licht. Planta 47: 127–158.
• Näf U. 1959. Control of antheridium formation in the fern Anemia phyllitidis. Nature (London) 184: 798–800.
• Näf U., Nakanishi K., Endo M. 1975. On the physiology and chemistry of fern antheridiogens. Bot. Review 41: 315–359.
• Olszewska M.J. 1978. Chromatin condensation in late G2 phase as a factor related to the earlier initiation of mitosis in successive generations of antheridial filaments of Chara vulgaris L. Microscopica Acta 80: 115–126.
• Olszewska M.J., Marciniak K. 1980. Role of SS bridges in mitotic condensation of chromatin. Folia Histochem. Cytochem. 18: 23–32.
• Raghavan V. 1993. Differential expression of nuclear and plastid genes induced by red light and gibberellic acid during germination of spores of the fern Anemia phyllitidis. Am. J. Bot. 80: 385–390.
• Schraudolf H. 1962. Die Wirkung von Phytohormonen auf Keimung und Entwicklung von Farnprothallien. I. Auslosung der Antheridienbildung und Dunkelkeimung bei Schizaeaceen durch Gibberellinsäure. Biol. Zentralbl. 81: 731–740.
• Schraudolf H. 1964. Relative activity of the gibberellins in the antheridium induction in Anemia phyllitidis. Nature 20: 98–99.
• Schraudolf H. 1966. Die Wirkung von Phytohormonen auf Keimung und Entwicklung von Farnprothallien II. Analyse der Wechselbeziehung und physiologischem Alter der Prothalliumzellen in Anemia phyllitidis. Planta (Berl.) 68: 335–352.
• Schraudolf H. 1967. Wirkung von Terpenderivaten (Helminthosporol, Helminthosporsäure, Dihydrohelminthosporsäure und Steviol) auf Die Antheridienbildung in Anemia phyllitidis. Planta (Berl.) 74: 188–193.
• Schraudolf H. 1987a. The effect of gabaculine on germination and gamethophyte morphogenesis of Anemia phyllitidis. Plant Cell. Physiol. 28: 53–60.
• Schraudolf H. 1987b. Antagonistic effects of abscisic acid and ABA-analogues on hormone-induced antheridium differentiation. J. Plant. Physiol. 131: 433–439.