Nuclear localization and binding affinity of STAT5b for the alpha2-macroglobulin gene promoter during rat liver development and the acute-phase response

• Autorzy: Mihailovic M. , Dinic S. , Bogojevic D. , Ivanovic-Matic S. , Uskokovic A. , Arambasic J. , Grigorov I. , Grdovic N. , Vidakovic M. , Martinovic V. , Petrovic M. , Poznanovic G.
• Języki publikacji: EN

Abstrakty

EN

Expression of the rat α2-macroglobulin (MG) gene undergoes dynamic changes throughout an individual's life and during the acute-phase (AP) response. Details of the participation of the STAT family of transcription factors in its control remain incompletely understood. Here we examined the involvement of STAT5b in MG gene expression during development and the AP response. Immuno-blot analysis revealed the highest nuclear level of STAT5b in the fetus and during postnatal development, whereas in the adult it decreased. Stimulation of MG expression during the AP response was accompanied by a decrease in STAT5b. Examination of STAT5b localization revealed that the relative concentrations of STAT5b were higher in the nuclear matrix than in the nuclear extract. Affinity chromatography with the extended promoter region of the MG gene (-825/+12), followed by immuno-blot analysis, revealed dynamic changes in STAT5b binding. The highest concentration of the promoter-binding form of STAT5b was observed in the fetus. As postnatal development progressed, the level of promoter-bound STAT5b decreased and in the adult liver it was the lowest. Stimulation of MG gene expression during the AP response in both the fetus and adult was accompanied by significantly decreased STAT5b binding to the MG promoter. The AP response was accompanied by lower levels of STAT5b serine and tyrosine phosphorylation in both fetus and adult. In the nuclear matrix derived from adult tissues, tyrosine phosphorylated species were completely absent. We conclude that developmental-stage differences in the mechanisms that determine STAT5b nuclear localization contribute to its activity in vivo.

Słowa kluczowe

EN

serum protein; gene promoter; phosphorylation; alpha2-macroglobulin; liver development; acute phase response; nuclear matrix; rat; nuclear extract

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2007
• Tom: 54
• Numer: 2
• Opis fizyczny: p.331-340,fig.,ref.

Twórcy

autor

Mihailovic M.

• Institute for Biological Research, Despot Stephen Blvd.142, 11060 Belgrade, Serbia

autor

Dinic S.

autor

Bogojevic D.

autor

Ivanovic-Matic S.

autor

Uskokovic A.

autor

Arambasic J.

autor

Grigorov I.

autor

Grdovic N.

autor

Vidakovic M.

autor

Martinovic V.

autor

Petrovic M.

autor

Poznanovic G.

Bibliografia

• Abbink JJ, Nuijens JH, Eerenberg AJ, Huijbregts CC, Strack van Schijndel RJ, Thijs LG, Hack CE (1991) Quantification of functional and inactivated alpha 2-macroglobulin in sepsis. Thromb Haemost 65: 32-39.
• Akira S, Nishio Y, Inoue M, Wang XJ, Wei S, Matsusaka T, Yoshida K, Sudo T, Naruto M, Kishimoto T (1994) Molecular cloning of APRF, a novel IFN-stimulated gene factor 3 p91-related transcription factor involved in the gp 130-mediated signaling pathway. Cell 77: 63-71.
• Aoki N, Matsuda T (2000) A cytosolic protein-tyrosine phosphatase PTP1B specifically dephosphorylates and deactivates prolactin-activated STAT5a and STAT5b. J Biol Chem 275: 39718-39726.
• Barrett AJ, Starkey PM (1973) The interaction of alpha2-macroglobulin with proteinases. Characteristics and specificity of the reaction, and a hypothesis concerning its molecular mechanism. Biochem J 133: 709-724.
• Baumann H, Gauldie J (1994) The acute phase response. Immunol Today 15: 74-80.
• Baumann H, Held WA, Berger FG (1984) The acute-phase response of mouse liver. Genetic analysis of major acute phase reactants. J Biol Chem 259: 566-573.
• Belgrader P, Siegel AJ, Berezney R (1991) A comprehensive study on the isolation and characterization of the HeLa S3 nuclear matrix. J Cell Sci 98: 281-291.
• Berezney R, Mortillaro MJ, Ma H, Wei X, Samarabandu J (1995) The nuclear matrix: A structural milieu for genomic function. Int Rev Cytol 162A: 1-65.
• Bidwell JP, van Wijnen AJ, Fey EG, Dworetzky S, Penman S, Stein JL, Lian JB, Stein GS (1993) Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components. Proc Natl Acad Sci USA 90: 3162-3166.
• Bode JG, Fischer R, Haussinger D, Graeve L, Heinrich PC, Schaper F (2001) The inhibitory effect of IL-1b on IL-6- induced a2-macroglobulin expression is due to activation of NF-kB. J Immunol 167: 1469-1481.
• Bogojevic D, Mihailovic M, Petrovic M, Dinic S, Ivanovic-Matic S, Poznanovic G (2003) Acute-phase-dependent binding affinity of C/EBPb from the nuclear extract and nuclear matrix towards the hormone response element of the a2- macroglobulin gene in rat hepatocytes. Gen Physiol Biophys 22: 279-285.
• Cox RA (1968) The use of guanidinium chloride in the isolation of nucleic acids. Methods Enzymol 12: 120-129.
• Dajee M, Fey GH, Richards JS (1998) Stat 5b and the orphan nuclear receptors regulate expression of the alpha2- macroglobulin (alpha2M) gene in rat ovarian granulosa cells. Mol Endocrinol 12: 1393-409.
• Darnell JEJ (1997) STATs and gene regulation. Science 277: 1630-1635.
• Dinic S, Ivanovic-Matic S, Bogojevic D, Mihailovic M, Poznanovic G (2000) C/EBPa and C/EBPb are persistently associated with the rat liver nuclear matrix throughout development and the acute phase response. Cell Biol Int 24: 691­698.
• Dinic S, Ivanovic-Matic S, Mihailovic M, Bogojevic D, Poznanovic G (2004) Expression of C/EBP8 in rat liver during development and the acute-phase response. Gen Physiol Biophys 23: 499-504.
• Dinic S, Bogojevic D, Petrovic M, Poznanovic G, Ivanovic-Matic S, Mihailovic M (2005) C/EBPa and C/EBPb regulate haptoglobin gene expression during rat liver development and the acute-phase response. Mol Biol Rep 32: 141-147.
• Fackelmayer FO (2004) Nuclear architecture and gene expression in the quest for novel therapeutics. Curr Pharm Des 10: 2851-60.
• Fletcher S, Thomas T, Schreiber G, Heinrich PC, Yeoh GCT (1988) The development of rat a2-macroglobulin. Eur J Biochem 171: 703-709.
• Fritz H, Jochum M, Geiger R, Duswald KH, Dittmer H, Kortmann H, Neumann S, Lang H (1986) Granulocyte proteinases as mediators of unspecific proteolysis in inflammation: a review. Folia Histochem Cytobiol24: 99-115.
• Fujitani Y, Nakajima K, Kojima H, Nakae K, Takeda T, Hirano T (1994) Transcriptional activation of the IL-6 response element in the junB promoter is mediated by multiple Stat family proteins. Biochem Biophys Res Commun 202: 1181­1187.
• Getzenberg RH (1994) Nuclear matrix and the regulation of gene expression: tissue specificity. J Cell Biochem 55: 22-31.
• Getzenberg RH, Coffey DS (1991) Interaction of tissue specific genes with tissue specific nuclear matrix proteins in rat sex accessory tissues. J Cell Biol 115: 121A.
• Glibetic M, Bogojevic D, Matic S, Sevaljevic Lj (1992) The expression of liver acute-phase protein genes during rat liver development and in response to inflammation of the dam. Differentiation 50: 35-40.
• Gonias SL (1992) a2-Macroglobulin: a protein at the interface of fibrinolysis and cellular growth regulation. Exp Hematol 20: 302-311.
• Gorski K, Carneiro M, Schibler U (1986) Tissue-specific in vitro transcription from the mouse albumin promoter. Cell 47: 767-776.
• Hendzel MJ, Sun JM, Chen HY, Rattner JB, Davie JR (1994) Histone acetyltransferase is associated with the nuclear matrix. J Biol Chem 269: 22894-22901.
• Herbst RS, Babbis LE (1990) Regulation of liver gene expression during development and regeneration. In Mechanisms of Differentiation (Fisher PB, Phil M, eds) pp 16-49. CRC Press, Boca Raton, FL.
• Hoel PG (1966) Introductions to Mathematical Statistics. pp 402-403. John Wiley and Sons, New York.
• Ito T, Tanahashi H, Misumi Y, Sakaki Y (1989) Nuclear factors interacting with an interleukin-6 responsive element of a2- macroglobulin gene. Nucleic Acids Res 17: 9425-9435.
• Ivanovic-Matic S, Dinic S, Vujosevic M, Poznanovic G (2000) The protein composition of the hepatocyte nuclear matrix is differentiation-stage specific. IUBMB Life 49: 511-517.
• Jackson DA (1997) Chromatin domains and nuclear compartments: establishing sites of gene expression in eukaryotic nuclei. Mol Biol Rep 24: 209-220.
• Kadonaga JT, Tjian R (1986) Affinity purification of sequence-specific DNA binding proteins. Proc Natl Acad Sci USA 83: 5889-5893.
• Kafatos FC, Jones CW, Efstradiadis A (1979) Determination of nucleic acid sequence homologies and relative concentration by a dot hybridization procedure. Nucleic Acids Res 7: 1541-1552.
• Kaufmann SH, Shaper JH (1984) A subset of non-histone nuclear proteins reversibly stabilized by the sulfhydryl cross- linking reagent tetrathionate. Exp Cell Res 155: 477-495.
• Khan MM, Shibuya Y, Kambara T, Yamamoto T (1995) Role of alpha2-macroglobulin and bacterial elastase in guinea-pig pseudomonal septic shock. Int J Exp Pathol 76: 21-28.
• Kunz D, Zimmermann R, Heisig M, Heinrich PC (1989) Identification of the promoter sequence involved in the interleukin- 6 dependent expression of the rat a2-macroglobulin gene. Nucleic Acids Res 17: 1121-1138.
• Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680­685.
• Laurell CB (1972) Electrophoretic and electroimmunochemical analysis of proteins. Scand J Clin Lab Invest 29: 21-37.
• Lemon B, Tjian R (2000) Orchestrated response: a symphony of transcription factors for gene control. Genes Dev 14: 2551­2569.
• Lindenmuth DM, van Wijnen AJ, Hiebert S, Stein JL, Lian JB, Stein GS (1997) Subcellular partitioning of transcription factors during osteoblast differentiation: Developmental association of the AML/CBFa/PEBP2a-related transcription factor-NMP-2 with the nuclear matrix. J Cell Biochem 66: 123-132.
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurements with the Folin phenol reagent. J Biol Chem 193: 265-275.
• Nickerson J (2001) Experimental observations of a nuclear matrix. J Cell Sci 114: 463-474.
• Noda C, Ichihara A (1993) Regulation of liver-specific gene expression. Cell Struct Funct 18: 189-194.
• Northemann W, Heisig M, Kunz D, Heinrich PC (1985) Molecular cloning of cDNA sequence for rat a2-macroglobulin and measurement of its transcription during experimental inflammation. J Biol Chem 260: 6200-6205.
• Okubo H, Miyanaga O, Nagano M, Ishibashi H, Kudo J, Ikuta T, Shibata K (1981) Purification and immunological determination of rat a2-macroglobulin in serum from injured rats. Biochim Biophys Acta 668: 257-267.
• Panrucker DE, Lai PCW, Lorscheider FL (1983) Distribution of acute-phase alpha2-macroglobulin in rat fetomaternal compartments. Am J Physiol 245: E138-E142.
• Reyes JC, Muchardt C, Yaniv M (1997) Components of the human SWI/SNF complex are enriched in active chromatin and are associated with the nuclear matrix. J Cell Biol 137: 263-274.
• Ripperger JA, Fritz S, Richter K, Hocke GM, Lottspeich F, Fey GH (1995) Transcription factor Stat 3 and Stat 5b are present in rat liver nuclei late in an acute phase response and bind interleukin-6 response elements. J Biol Chem 270: 29998­30006.
• Schaefer U, Brucker B, Elbers A, Neugebauer E (2004) The capacity of alpha2-macroglobulin to inhibit an exogenous protease is significantly increased in critically ill and septic patients. Shock 22: 16-22.
• Sehgal PB (2003) Plasma membrane rafts and chaperones in cytokine/STAT signaling. Acta Biochim Polon 50: 583-594.
• Sjakste NI, Sjakste TG (2001) Transcription factors and the nuclear matrix. Mol Biol 35: 627-635.
• Sottrup-Jensen L, Sand O, Kristensen L, Fey GH (1989) The alpha-macroglobulin bait region. Sequence diversity and localization of cleavage sites for proteinases in five mammalian alpha-macroglobulins. J Biol Chem 264: 15781-15789.
• Standke G, Meier V, Groner B (1994) Mammary gland factor activated by prolactin in mammary epithelial cells and acute- phase response factor activated by interleukin-6 in liver cells share DNA binding and transactivation potential. Mol Endocrinol 8: 469-477.
• Stein GS, Zaidi SK, Braastad CD, Montecino M, van Wijnen AJ, Choi JY, Stein JL, Lian JB, Javed A (2003) Functional architecture of the nucleus: Organizing the regulatory machinery for gene expression, replication, and repair. Trends Cell Biol 13: 584-592.
• Stuurman N, Meijne AML, van der Pol AJ, de Jong L, van Driel R, van Renswoude J (1990) The nuclear matrix from cells of different origin. Evidence for a common set of matrix proteins. J Biol Chem 265: 5460-5465.
• Sevaljevic Lj, Petrovic M, Bogojevic D, Savic J, Pantelic D (1989) Acute-phase response to scalding: changes in serum properties and acute-phase protein concentrations. Circ Shock 28: 293-307.
• Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
• van Wijnen AJ, Bidwell JP, Fey EG, Penman S, Lian JB, Stein JL, Stein GS (1993) Nuclear matrix association of multiple sequence-specific DNA binding activities related to SP-1, ATF, CCAAT, C/EBP, OCT-1, and AP-1. Biochemistry32: 8397-8402.
• Wegenka UM, Lutticken C, Buschmann J, Yuan J, Lottspeich F, Muller-Esterl W, Schindler C, Roeb E, Heinrich PC, Horn F (1994) The interleukin-6-activated acute-phase response factor is antigenically and functionally related to members of the signal transducer and activator of transcription (STAT) family. Mol Cell Biol 14: 3186-3196.
• Wen Z, Darnell JJE (1997) Mapping of Stat3 serine phosphorylation to a single residue (727) and evidence that serine phosphorylation has no influence on DNA binding of Stat1 and Stat3. Nucleic Acids Res 25: 2062-2067.
• Wen Z, Zhong Z, Darnell JJE (1995) Maximal activation of transcription by Stat 1 and Stat3 requires both tyrosine and serine phosphorylation. Cell 82: 241-250.
• Wesoly J, Szweykowska-Kulinska Z, Bluyssen HAR (2007) STAT activation and differential complex formation dictate selectivity of interferon responses. Acta Biochim Polon 54: 27-38.
• Zeng C, McNeil S, Pockwinse J, Nickerson J, Shopland L, Lawrence JB, Penman S, Lian JB, Stein GS, Hiebert SW (1998) Intranuclear targeting of AML/CBFa regulatory factors to nuclear matrix-associated transcriptional domains. Proc Natl Acad Sci USA 95: 1585-1589.
• Zhang X, Darnell JEJ (2001) Functional importance of Stat3 tetramerization in activation of the a2-macroglobulin gene. J Biol Chem 276: 33576-33581.
• Zhang Z, Fuller GM (2000) Interleukin 1b inhibits interleukin 6-mediated rat g-fibrinogen gene expression. Blood 96: 3466­3472.