Effect of vitamin E derivative [U83836E] on membranes of rat liver cells after methanol intoxication

• Autorzy: Skrzydlewska E , Dobrzynska I. , Kasacka I. , Figaszewski Z.
• Języki publikacji: EN

Abstrakty

EN

Methanol oxidation in vivo to formaldehyde and next to formate, which takes place mainly in the liver, is accompanied by free radicals generation. The goal of this paper is to desribe the effect of vitamin E derivative (U83836E) on rat liver cell membranes during methanol intoxication (3.0 g/kg body weight). This study has been concerned with measurement of lipid peroxidation and surface charge density of liver cells and activity of lysosomal enzyme - cathepsin B - in cytosol of liver cells and the blood serum. An ultrastructural study of liver cells has also been conducted. It has been proven that methanol administration causes an increase in lipid peroxidation products (by 33%) as well as in surface charge density (by 64%) of the liver cells. This might have resulted from the membrane liver cell damage visible in the electron microscope and in leak of cathepsin B into cytosol (increase in activity by 28%) as well as the leak of cathepsin B from cytosol into the blood (increase in serum activity by 27%). Administration of U83836E to rats intoxicated with methanol partially prevented the above changes. Obtained results suggest that U83836E acts as an effective antioxidant in methanol intoxication.

Słowa kluczowe

EN

cell; vitamin E derivative; membrane; methanol; liver; intoxication; rat; lipid peroxidation

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2001
• Tom: 10
• Numer: 2
• Opis fizyczny: p.95-100,fig.,ref.

Twórcy

autor

Skrzydlewska E

• Medical Academy of Bialystok, 15-230 Bialystok 8, Poland

autor

Dobrzynska I.

autor

Kasacka I.

autor

Figaszewski Z.

Bibliografia

• 1. LIESIVUORI J., SAVOLAINEN H. Methanol and formic acid toxicity: biochemical mechanisms. Pharmacol. Toxicol. 69,157, 1991.
• 2. BOLT H.M. Experimental toxicology of formaldehyde. J. Cancer Res. Clin. Oncol. 113, 305, 1987.
• 3. KATO S, KAWASE T., ALDERMAN J., INATORI N., LIEBER C.S. Role of xanthine oxidase in ethanol – induced lipid peroxidation. Gastroenterology 98, 203, 1990.
• 4. CHIU D., KUYPERS F., LUBIN B. Lipid peroxidation in human red cell. Seminars in Hematol. 26, 257, 1989.
• 5. SKRZYDLEWSKA E., FARBISZEWSKI R. Antioxidant status of liver, erythrocytes and blood serum of rats in acute methanol intoxication. Alcohol 14, 431, 1997.
• 6. BENGA G., HOLMES R.P. Interactions between components in biological membranes and their implications for membrane function. Prog. Biophys. Molec. Biol. 43, 195, 1984.
• 7. DOLOWY K. Bioelectrochemistry of cell surfaces. Prog. Surf. Sci. 15, 245,1984.
• 8. HALL E.D. Novel inhibitors of iron - dependent lipid per oxidation for neurode generative disorders. Ann. Neurol. 32, 137,1992.
• 9. MCMARTIN K.E., MAKAR A.B, MARTIN-AMAT G, PALESE M., TEPHLY T.R. Methanol poisoning. The role of formic acid in the development of metabolic acidosis in the monkey and the reversal by 4-methylpyrazole. Biochem. Med. 13, 319, 1975.
• 10. TAWATARI T., KAWABATA Y, KATUNUMA N. Crystalization and properties of cathepsin B from rat liver. Eur. J. Biochem. 102, 279, 1979.
• 11. MCCORD J.M., FRIDOVICH I. The generation of superoxide radicals during the autooxidation of hemoglobin. J. Biol. Chem. 247, 6960, 1972.
• 12. ESTERBAUER H., LANG J, ZADRAVEC S., SLATER T.F. Detection of malonaldehyde by high-performance liquid chromatography. Methods Enzymol. 105, 319, 1984.
• 13. KRYSINSKI P., TIEN H.T. Membrane electrochemistry. Prog. Surf. Sci. 23(4), 317, 1986.
• 14. MINC S, KRYSINSKI P. Electrophoretic studies on adsorption of tetramethylamonium cation (TMA+) on human erythrocytes in vitro. Bioelectrochem. Bioenerg. 5, 247, 1978.
• 15. SKRZYDLEWSKA E., DOBRZYNSKA I., FIGASZEWSKI Z. Oxidative processes and changes of surface charge density of erythrocytes in rat during methanol intoxication. Cell. Mol. Biol. Lett. 2, 345, 1997.
• 16. KLEMM W.R. YURTTAS L. The dehydration theory of alcohol intoxication. In: Watson, R.R. ed.: Drug and Alcohol Abuse Reviews, vol. 3. The Human Press Inc. pp 169-185, 1992.
• 17. TOME D., NAULET N, KOZLOWSKI A. C13-n.m.r. characterization of formaldehyde bonds in model mixtures and proteins containing lysine. Int. J. Peptide Protein Res. 25, 258,1985.
• 18. GALEMBECK F, RYAN J.R., FEENEY R.E. Reaction of proteins with formaldehyde in the presence and absence of sodium borohydride. J. Agric. Food Chem. 25, 238, 1977.
• 19. SKRZYDLEWSKA E., FARBISZEWSKI R. Diminished antioxidant defense potential of liver, erythrocytes and serum from rats with subacute methanol intoxication. Vet. Hum. Toxicol. 38, 429, 1996.
• 20. ESTERBAUER H., SCHAUR R.J., ZOLLER J. Chemistry and biochemistry of 4 hydroxynonenal, malondialdehyde and related aldehydes. Free Radic. Biol. Med. 11, 81, 1991.
• 21. HEGNER D. Age-dependence of molecular and functional changes in biological membrane properties. Mech. Ageing Dev. 14, 101, 1980.
• 22. BENEDETTI A. COMPORT! M., ESTERBAUER H. Identification of 4-hydroxynonenal as a cytotoxic product originating from the peroxidation of liver microsomal lipid. Biochem. Biophys. Acta. 620, 281, 1980.
• 23. KASACKA I., SKRZYDLEWSKA E. Parenchymal cell mitochondria in the rats after methanol intoxication. Folia Histoch. Et. Cytobiol. 37(2), 111, 1999.
• 24. SKRZYDLEWSKA E., FARBISZEWSKI R. Trolox-derivative antioxidant protects against methanol-induced damage. Fundam. Clin. Pharmacol. 11, 460, 1997.
• 25. BARCLAY L.R., VINQVIST M.R. Membrane peroxidation: inhibiting effects of water-soluble antioxidants on phospholipids of different charge types. Free Radic. Biol. Med. 16(6), 779, 1994.