Influence of the therapy of laying hens with selected antibiotics on the presence of Salmonella enteritidis in the contents of the eggs

• Autorzy: Kolasa A , Rzedzicki J. , Skowron M.
• Języki publikacji: EN

Abstrakty

EN

One of the methods that can reduce the transmission of Salmonella from the reproductive tract to eggs can be the application of antibiotic therapy of infected hens. Studies characterizing the antibiotics effectiveness in salmonellosis therapy have shown that the therapy can only decrease the number of the bacterial population but it does not fully eliminate bacteria. The aim of the study was the assessment of the therapy of hens with selected antibiotics on the presence Salmonella Enteritidis in the content of eggs. The investigations were conducted in two stages. In the first stage the hens were infected experimentally with Salmonella Enteritidis. In the second stage the hens that had been infected were treated with selected antibiotics (enrofloxacine, norfloxacin, flumequine, amoxycillin and amoxicillin-clavulanic acid). In the present study S. Enteritidis was isolated from eggs that were laid during a 12 to 24 day interval post inoculation in all experimental groups but at individual days the quantity of infected eggs varied. On the 24th day post inoculation there was the highest amount (29.4%) of all contaminated eggs. After antibiotic therapy in some groups of hens there were no infected eggs. This pertained to the birds which were treated with enrofloxacine and norfloxacin. The result of the therapy with amoxycilln, amoxicillin-clavulanic acid and flumequine in the other groups was only the reduction of the quantity of infected eggs. The findings presented above demonstrate that the antibiotic therapy of hens infected with Salmonella Enteritidis has not always been effective in the elimination of these bacteria from the tissues of the reproductive tract but the therapy with enrofloxacine and norfloxacin can eliminate the possibility of salmonellas transmission into the contents of the eggs. Moreover, these examinations have shown the correlation between the infection of reproductive tract organs (ovary, oviduct) and the contents of eggs (yolk, white) that were laid by hens infected with Salmonella Enteritidis.

Słowa kluczowe

EN

hen; laying hen; therapy; antibiotic; egg; egg content; Salmonella enteritidis; antibiotic effectiveness; infected hen

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2007
• Tom: 63
• Numer: 10
• Strony: 1168-1171
• Opis fizyczny: p.1168-1171,fig.,ref.

Twórcy

autor

Kolasa A

• University of Agriculture in Lublin, Gleboka 30, 20-612 Lublin, Poland

autor

Rzedzicki J.

autor

Skowron M.

Bibliografia

• 1.Anon.: National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals Second Edition: Approved Standard M31-A2. NCCLS, Wayne. PA., USA 2002.
• 2.Chylak J.: Skutecznoœæ i farmakokinetyka wybranych chinolonów. Pol. Tyg. Lek. 1993, 48, 860-863.
• 3.De Buck J., Pasmans F., Van Immerseel F., Haesebrouck F., Ducatelle R.: Tubular glands of the isthmus are the predominant colonization site of Salmonella Enteritidis in the upper oviduct of laying hens. Poult. Sci. 2004, 83, 325-358.
• 4.Gustafson R. H., Bowen R. E.: Antibiotic use in animal agriculture. J. Appl. Microbiol. 1997, 83, 531-541.
• 5.Hoop R. K., Pospischil A.: Bacteriological, serological, histological and immunohistochemical findings in laying hens with naturally acquired Salmonella enteritidis phage type 4 infection. Vet. Rec. 1993, 133, 391-393.
• 6.Humphrey T. J., Baskerville A., Mawer S., Rowe B., Hopper S.: Salmonella enteritidis phage type 4 from the contents of intact eggs: a study involving naturally infected hens. Epidemiol. Infect. 1989, 103, 415-423.
• 7.Humphrey T. J., Cruickshank J. G., Rowe B.: Salmonella enteritidis phage type 4 and hens' eggs. Lancet i 1989, 281.
• 8.Kałużewski S.: Zasady i metodyka oznaczania wrażliwości bakterii na chemioterapeutyki w rutynowych badaniach diagnostycznych. PZH, Warszawa 1976.
• 9.Keller L. H., Benson C. E., Krotec K., Eckroade R. J.: Salmonella Enteritidis colonization of the reproductive tract and forming and freshly laid eggs. Infect. Immun. 1995, 63, 2443-2449.
• 10.Memish Z. A., Venkatesh S., Shibl A. M.: Impact of travel on international spread of antimicrobial resistance. Internat. J. Antimicrob. Agents 2003, 21, 135-142.
• 11.Miyamoto T., Baba E., Tanaka T., Sasai K., Fukata T., Arakawa A.: Salmonella Enteritidis contamination of eggs from hens inoculated by vaginal, cloacal and intravenous routes. Avian Dis. 1997, 41, 296-303.
• 12.Okamura M., Kamijima Y., Miyamoto T., Tani H., Sasai K., Baba E.: Differences among six Salmonella serovars in abilities to colonize reproductive organs and contaminate eggs in laying hens. Avian Dis. 2001, 45, 61-69.
• 13.Pacifici G. M., Viani A.: Methods of determining plasma and tissue binding of drugs: pharmacokinetic consequences. Clin. Pharmacokinet. 1992, 23, 449-468.
• 14.Rahn K., De Grandis S. A., Clarke R. C., McEven S. A., Galan J. E., Ginocchio C., Curtiss R. 3rd, Gyles C. L.: Amplification of an invA gene sequence of Salmonella typhimurium by polymerase chain reaction as a specific method of detection of Salmonella. Mol. Cell Probes 1992, 6, 271-279.
• 15.Timoney J. F., Shivaprasad H. L., Baker R. C., Rowe B.: Egg transmission after infection of hens with Salmonella Enteritidis phage type 4. Vet. Rec. 1989, 125, 600-601.
• 16.Trziszka T.: Odchylenia jakoœciowe w surowcu jajczarskim wywołane kontaminacją pasz mikotoksynami oraz wpływem kokcydiostatyków i antybiotyków. Medycyna Wet. 1994, 50, 26-29.