Correlation of tests for detection of Borrelia burgdorferi sensu lato infection in patients with diagnosed borreliosis

• Autorzy: Chmielewska-Badora J. , Cisak E. , Wojcik-Fatla A. , Zwolinski J. , Buczek A. , Dutkiewicz J.
• Języki publikacji: EN

Abstrakty

EN

A group of 180 patients with diagnosed Lyme borreliosis were examined for the presence of infection with Borrelia burgdorferi sensu lato (B. burgdorferi s.l.) by serologic tests with B. burgdorferi s.l. antigens (IgM-ELISA, IgG-ELISA, IgM-immunoblot, IgG-immunoblot) and by polymerase chain reaction (PCR, nested-PCR) for detection of B. burgdorferi s.l. DNA in peripheral blood. A total of 61.7%, 53.9%, 62.2%, and 59.4% of the examined patients’ sera showed positive or borderline results in the serologic tests IgM-ELISA, IgG-ELISA, IgM-immunoblot, and IgG immunoblot, respectively. The results of the tests IgM-ELISA and IgM-immunoblot were significantly correlated (p<0.001). A higher degree of the correlation (p<0.000001) was found at the comparison of results obtained with IgG-ELISA and IgG-immunoblot. The correlation between the positive findings in the IgM-ELISA and detection with IgM-immunoblot the diagnostically important B. burgdorferi s.l. OspC surface protein was relatively low but statistically significant (0.01B. burgdorferi s.l. antigen, the VlsE protein (p<0.000001). The presence of B. burgdorferi s.l. DNA was found by PCR in 20 out 180 examined blood samples (11.1%). No correlation was found to exist between the PCR results and the results of any of the serologic tests for detection of anti B. burgdorferi s.l. antibodies of IgM class. PCR results correlated significantly at a relatively low level (0.01Borrelia burgdorferi sensu lato.

Słowa kluczowe

EN

correlation; serological test; Lyme borreliosis; VlsE protein; polymerase chain reaction; Borrelia burgdorferi; OspC protein

• Wydawca: -
• Czasopismo: Annals of Agricultural and Environmental Medicine
• Rocznik: 2006
• Tom: 13
• Numer: 2
• Opis fizyczny: p.307-311,ref.

Twórcy

autor

Chmielewska-Badora J.

• Institute of Agricultural Medicine, Jaczewskiego 2, 20-090 Lublin, Poland

autor

Cisak E.

autor

Wojcik-Fatla A.

autor

Zwolinski J.

autor

Buczek A.

autor

Dutkiewicz J.

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