Relationship between the molecular typing of Campylobacter strains and the prevalence of their virulence genes

• Autorzy: Wieczorek K
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to evaluate and compare two genotyping methods for the examination of genetic diversity in C. jejuni and C. coli strains isolated from poultry. Moreover, the molecular identification of putative virulence factors was additionally included to establish a correlation between the isolates. Both typing methods, RAPD-PCR and ERIC-PCR, were found to have a high discriminatory power, D=0.95 and 0.94, respectively. The isolates were clustered to 28 groups by RAPD-PCR and to 29 by ERIC- PCR. Sometimes the ERIC or RAPD assays were able to further discriminate the isolates, which had the same virulence profile generated by the second technique. These PCR-based differentiation methods demonstrated the potential for initial rapid epidemiology studies, and when used in conjunction with distribution of virulence markers provide a high resolution strategy for accurately defining subtypes of Campylobacter.

Słowa kluczowe

EN

genotyping method; genetic diversity; Campylobacter jejuni; Campylobacter coli; poultry; molecular identification; putative factor; virulence gene; virulence marker; virulence profile

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 2
• Strony: 193-198
• Opis fizyczny: p.193-198,fig.,ref.

Twórcy

autor

Wieczorek K

• National Veterinary Research Institute, 24-100 Pulawy, Poland

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