The influence of LTS-4, a saponoside from Lysimachia thyrsiflora L., on human skin fibroblasts and human melanoma cells

• Autorzy: Galanty A , Michalik M. , Sedek L. , Podolak I.
• Języki publikacji: EN

Abstrakty

EN

We investigated the effect of a triterpene saponoside from Lysimachia thyrsiflora L. upon the viability, proliferation, morphology and cell motility of human melanoma HTB-140 cells and human skin fibroblasts (HSFs). The compound, denoted LTS-4, decreased the viability and rate of cell growth of both cell types in a time-and dose-dependent manner, and proved cytotoxic against cancer cells at significantly lower concentrations than for fibroblasts. LTS-4 also affected the morphology of the examined cells, causing vacuolisation and actin cytoskeleton disintegration, and had an inhibitory effect on the tumour cell motility.

Słowa kluczowe

EN

triterpene saponoside; cytotoxicity; Lysimachia thyrsiflora; melanoma; fibroblast; secondary metabolite; triterpenoid saponin

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2008
• Tom: 13
• Numer: 4
• Strony: 585-598
• Opis fizyczny: p.585-598,fig.,ref.

Twórcy

autor

Galanty A

• Jagiellonian University, Medyczna 9, 30-688 Krakow, Poland

autor

Michalik M.

autor

Sedek L.

autor

Podolak I.

Bibliografia

• 1.Xiao, K., Yi, Y.H., Wang, Z.Z., Tang, H.F., Li, Y.Q. and Lin, H.W. A cytotoxic triterpene saponin from the root bark of Aralia dasyphylla. J. Nat. Prod. 62 (1999) 1030-1032.
• 2. Barthomeuf, C., Debiton, E., Mshvildadze, V., Kemertelidze, E. and Balansard, G. In vitro activity of hederacolchisid A1 compared with other saponins from Hedera colchica against proliferation of human carcinoma and melanoma cells. Planta Med. 68 (2002) 672-675.
• 3. Marquina, S., Maldonado, N., Garduno-Ramirez, M.L., Aranda, E., Villarreal, M.L., Navarro, V., Bye, R., Delgado, G. and Alvarez, L. Bioactive oleanolic acid saponins and other constituents from the roots of Viguiera decurrens. Phytochemistry 56 (2001) 93-97.
• 4. Podolak, I., Galanty, A., Trojanowska, D. and Janeczko, Z. Evaluation of cytotoxic and antimicrobial activity of Lysimachia thyrsiflora. 3rd International Symposium on Natural Drugs Proceedings, Naples, 2003, 199-200.
• 5. Karpova, V.I., Kintya, P.K. and Chirva, V.Y. Structure of saponin A from Naumburgia thyrsiflora. Khim. Prir. Soedin. 11 (1975) 364-366.
• 6. Kintya, P.K., Karpova, V.I. and Chirva, V.Y. Saponin from Naumburgia thyrsiflora. Khim. Prir. Soedin. 11 (1985) 520-521.
• 7. Yasukawa, K., Taira, T. and Takido, M. Flavonol gycosides from Lysimachia thyrsiflora. Biochem. Syst. Ecol. 25 (1997) 73-74.
• 8. Podolak, I., Janeczko, Z., Galanty, A., Michalik, M. and Trojanowska, D. A triterpene saponin from Lysimachia thyrsiflora L. Acta Pol. Pharm. – Drug Res. 64 (2007) 39-43.
• 9. Drukała, J., Rajwa, B. and Pietrzkowski, Z. Effect of daunomycin on expression of CD44H protein in HTB140 melanoma cells in vitro. Anticancer Res. 21 (2001) 3793-3798.
• 10. Szydłowska, H., Zaporowska, E., Kuszlik-Jochym, K., Korohoda, W. and Branny, J. Membranolytic activity of detergents as studied with cell viability tests. Folia Histochem. Cytochem. 16 (1978) 69-78.
• 11. Korohoda, W. and Madeja, Z. Contact of sarcoma cells with aligned fibroblasts accelerates their displacement, computer assisted analysis of tumour cell locomotion in co-culture. Biochem. Cell. Biol. 75 (1997) 263-276.
• 12. Friedl, P., Noble, P.B. and Zänker, K.S. Lymphocyte locomotion in three dimensional collagen gels. Comparison of three quantitative methods for analysis cell trajectories. J. Immunol. Methods 165 (1993) 157-165.
• 13. Sroka, J., Madeja, Z., Galanty, A., Michalik, M., Przestalski, S., Rakoczy, L. and Korohoda, W. Trimethyltin inhibits the chemotaxis of Dictyostelium discoideum amoebae. Europ. J. Protistol. 37 (2001) 313-326.
• 14. Sroka, J., Kamiński, R., Michalik, M., Madeja, Z., Przestalski, S. and Korohoda, W. The effect of triethyllead on the motile activity of walker 256 carcinosarcoma cells. Cell. Mol. Biol. Lett. 9 (2004) 15-30.
• 15. Seeman, P., Cheng, D. and Iles, G.H. Structure of membrane holes in osmotic and saponin hemolysis. J. Cell. Biol. 56 (1974) 519-527.
• 16. Shany, S., Bernheimer, A.W., Grushoff, P.S. and Kim, K.S. Evidence for membrane cholesterol as common binding site for cereolysin, streptolysin O and saponin. Mol. Cell. Biochem. 3 (1974) 179-186.
• 17. Galeotti, T., Borello, S., Minotti, G. and Masotti, L. Membrane alternations in cancer cells: the role of oxy radicals. Ann. NY Acad. Sci. 488 (1986) 468-480.
• 18. Tugnoli, V., Trinchero, A. and Tosi, M.R. Evaluation of the lipid composition of human healthy and neoplastic renal tissues. Ital. J. Biochem. 53 (2004) 169-182.
• 19. Pierzchalska, M., Michalik, M., Stępień, E. and Korohoda, W. Changes in morphology of human skin fibroblasts induced by local anaesthetics: role of actomyosin contraction. Eur. J. Pharm. 358 (1998) 235-244.
• 20. Michalik, M., Pierzchalska, M., Pabiańczyk-Kulka, A. and Korohoda, W. Procaine-induced enhancement of fluid-phase endocytosis and inhibition of exocytosis in human skin fibroblasts. Eur. J. Pharm. 475 (2003) 1-10.
• 21. Pollard, T.D. and Cooper, J.A. Actin and actin-binding proteins: a critical evaluation of mechanisms and functions. Annu. Rev. Biochem. 55 (1986) 987-1035.
• 22. Osborn, M., Born, T., Koitsch, H.J. and Weber, K. Stereo immunofluorescence microscopy: I. Three-dimensional arrangement of microfilaments, microtubules and tonofilaments. Cell 14 (1978) 477-488.
• 23. Buendia, B., Bre, M.-H. and Karsenti, E. Cytoskeletal control of centrioles movement during the establishment of polarity in Madin-Darby canine kidney cells. J. Cell. Biol. 110 (1990) 1123-1135.
• 24. Baumann, E., Stoya, G., Völkner, A., Richter, W., Lemke, C. and Linss, W. Hemolysis of human erythrocytes with saponin affects the membrane structure. Acta Histochem. 102 (2000) 21-35.
• 25. Shinkai, K., Akedo, H., Mukai, M., Imamura, F., Isoai, A., Kobayashi, M. and Kitagawa, I. Inhibition of in vitro tumor cell invasion by ginsenoside Rg3. Jpn. J. Cancer Res. 87 (1996) 357-362.
• 26. Wakabayashi, C., Hasegawa, H., Murata, J. and Saiki, I. In vivo antimetastatic action of ginseng protopanaxadiol saponins is based on their intestinal bacterial metabolites after oral administration. Oncol. Res. 9 (1997) 411-417.
• 27. Wei-Wei, Ch., Chia-Yu, Y., Tzu-Wen, L., Peng-Hui, W. and Ying-Chieh, T. Soyasaponin I decreases the expression of α2,3-linked sialic acid on the cell surface and supresses the metastatic potential of B16F10 melanoma cells. Biochem. Biophys. Res. Commun. 341 (2006) 614-619.