Warianty tytułu
The influence of antibiotics on the appearance of magnolia stunting symptoms
Języki publikacji
Abstrakty
Treatment of diseased magnolia plants with oxytetracycline, baytril and tylan did not reduce the number of symptomatic plants. However, the sprays with antibiotics promoted the shoot growth, development of symptomless leaves and flower buds. The most efficient were baytril at the concentration of 500 ppm, tylan 200 ppm and oxytetracycline at the concentration of 500 or 1000 ppm. Lower concentrations of baytril and oxytetracycline were not so much effective; higher 'concentration of tylan decreased the magnolia shoot growth. The effect of antibiotics lasted one season. Ali the antibiotic treated and control plants, randomly sted by PCR-RFLP, showed the presence of A Y (16SrI) phytoplasma and some f them were affected with phytoplasma related with apple proliferation hytoplasma group (16SrX). The obtained results indicated that l) magnolia is a natural host plant of phytoplasmas belonging to aster yellows phytoplasma group d apple proliferation group and 2) they support the suggestion that phytoplasmas the casual agents of magnolia stunting disease.
Traktowanie chorych magnolii roztworami oksytetracykliny, baytrilu i tylanu miało dodatni wpływ na wzrost pędów, liczbę liści bez symptomów oraz zawiązywanie pąków kwiatowych. Największy wpływ miały baytril w stężeniu 500 lub 1000 ppm, tylan 200 ppm i oksytetracyklina 500 lub 1000 ppm. Niższe stężenia baytrilu i oksytetracykliny nie były tak efektywne, zaś wyższa dawka tylanu hamowała rozwój pędów. Pozytywny wpływ antybiotyków obserwowano tylko w pierwszym roku ich stosowania. Wszystkie losowo wybrane i testowane przy pomocy analizy PCR-RFLP magnolie, zarówno traktowane antybiotykami jak i kontrolne, wykazały obecność fitoplazm z grupy żółtaczki astra (16SrI). Niektóre krzewy były porażone także fitoplazmą z grupy proliferacji jabłoni (16SrX). Wyniki niniejszych badań wykazały więc, że 1). magnolia jest naturalnym gospodarzem fitoplazm z grup żółtaczki astra i proliferacji jabłoni, oraz 2). potwierdzają przypuszczenie, że fitoplazmy są przyczyną choroby karłowatości magnolii.
Słowa kluczowe
Wydawca
Czasopismo
Rocznik
Tom
Numer
Strony
107-117
Opis fizyczny
s.107-117,rys.,fot.,bibliogr.
Twórcy
autor
- Instytut Sadownictwa i Kwiaciarstwa, ul.Pomologiczna 18, 100 Skierniewice
autor
Bibliografia
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- Andersen M.T., Вeever R.E., Sutherland P.W., Forster R.L., 2001. Association of "Candidatus Phytoplasma australiense" with sudden decline of cabbage tree in New Zealand. Plant Dis. 85: 462-469.
- Вradel B.G., Preil W., Jeske H., 2000. Remission of the free-branching pattern of Euphorbia pulcherrima by tetracycline treatment. J. Phytopathol. 148: 587-590.
- Davies R.E., Whitсomb R.F., Steere R.L., 1968. Remission of aster yellows disease by antibiotics. Science 161: 793-794.
- Doi Y., Teranaka M., Yоrа K., Asuуamа Н., 1967. Mycoplasma- or PLT grouplike microorganisms found in the phloem elements of plants infected with mulberry dwarf, potato witches' broom, aster yellows, or paulownia witches' broom. Ann. Phytopathol. Soc. Japan 33: 259-266.
- Freitag J.H., Smith S.H., 1969. Effects of tetracyclines on symptom expression and leafhopper transmission of aster yellows. Phytopathology 59: 1820-1823.
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- Kamińska M., Śliwa H., Rudzińska-Langwald A., 2001. The association of phytoplasma with stunting, leaf necrosis and witches, broom symptoms in magnolia plants. J. Phytopathol. 149: 719-724.
- Kirkpatrick В., Smart C., Blomquist C., Guerra L., Harrison N., Ahrens U., Lorenz K.H., Schneider В., Seemüller E., 1994. Identification of MLO strain-specific primers obtained from 16/23S spacer sequences. Int. Org. Mycoplasmol. Lett. 3: 261-262.
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- Lee R.F., 1987. Chemotherapy of cherry buckskin and peach yellow leafroll disease: an evaluation of two tetracycline formulations and methods of application. Plant Dis. 71: 119-121.
- Liао C.H., Chen T.A., 1981. In vitro susceptibility and resistance of two spiroplasmas to antibiotics. Phytopathology 71: 442-445.
- Marcone C., Ragozzino, Seemüller E., 1997. Witches' broom of Sarothamnus scoparius: a new disease associated with a phytoplasma related to the spartium witches' broom agent. J. Phytopathol. 145: 159-161.
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- Nуland G., Mоller W., 1973. Control of pear decline with a tetracycline. Plant Dis. Reptr. 57: 634-637.
- Schneider В., Seemüller E., Smart C.D., Kirkpatrick B.C., 1995. Phylogenic classification of plant pathogenic mycoplasmalike organisms or phytoplasmas. W: Razin S. and Tully J.G. (eds.) Molecular and Diagnostic Procedures in Mycoplasmology, vol. I: 369-380. Academic Press, San Diego.
- Seemüller E., Marcone C., Lauer U., Ragozzino A., Göschl M., 1998. Current status of molecular classification of the phytoplasmas. J. Plant Pathol. 80: 3-26.
- Shikata E., Teng W., Matsumoto T., 1969. Mycoplasma or PLT like microorganisms detected in leaves of sugarcane plants infected with white-leaf disease and the suppression of the disease symptoms the antibiotics of tetracycline group. J. Fac. Agr. Hokkaido Univ., Sapporo. 56: 79-90.
- Zawadzkа В., Kamińska M., 1975. The influence of oxytetracycline on the appearance of apple proliferation symptoms. Acta Hort.. 44: 19-22.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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