DNA damage and repair in lymphocytes of normal individuals and cancer patients: studies by the comet assay and micronucleus tests

• Autorzy: Palyvoda O. , Polanska J. , Wygoda A. , Rzeszowska-Wolny J.
• Języki publikacji: EN

Abstrakty

EN

A population study is reported in which the DNA damage induced by y-radiation (2 Gy) and the kinetics of the subsequent repair were estimated by the comet and micronucleus assays in isolated lymphocytes of 82 healthy donors and patients with head and neck cancer before radiotherapy. The parameters of background and radia­tion-induced DNA damage, rate of repair, and residual non-repaired damage were measured by comet assay, and the repair kinetics for every donor were com­puter-fitted to an exponential curve. The level of background DNA damage before ir­radiation measured by comet assay as well as the level of micronuclei were signifi­cantly higher in the head and neck cancer patient group than in the healthy donors, while the parameters of repair were widely scattered in both groups. Cancer patient group contained significantly more individuals, whose irradiated lymphocytes showed high DNA damage, low repair rate and high non-repaired DNA damage level. Lymphocytes of donors belonging to this subgroup showed significantly lower inhibi­tion of cell cycle after irradiation.

Słowa kluczowe

EN

ionizing radiation; normal patient; cancer patient; human lymphocyte; lymphocyte; micronucleus test; DNA repair; neck; DNA damage; head; tumour

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2003
• Tom: 50
• Numer: 1
• Opis fizyczny: p.181-190,fig.

Twórcy

autor

Palyvoda O.

• Center of Oncology, 44-100 Gliwice, Poland

autor

Polanska J.

autor

Wygoda A.

autor

Rzeszowska-Wolny J.

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