Expression of genes coding for animal virus glycoproteins in heterologous systems

• Autorzy: Bienkowska-Szewczyk K. , Szewczyk B.
• Języki publikacji: EN

Abstrakty

EN

The outermost layers of animal viruses are usually composed of glycoproteins. They are responsible not only for the entrance of viruses into, and release from host cells but also for the initial interaction of a viral particle with immunological defense of the host. It is therefore not surprising that many laboratories devote a lot of effort to study viral glycoproteins at the molecular level. Very often such studies are possible only after the introduction of a glycoprotein gene into a heterologous system. Expression of glycoprotein genes is usually obtained in mammalian or insect cells. Expression in mammalian cells yields viral glycoproteins with glycan chains indistinguishable from the original counterparts in virion particles but the level of synthesis of glycoproteins is very low. Vaccinia virus is the most common vector for expression in mammalian cells. It is easy to grow, the introduction of foreign genes is relatively simple and, due to the size of the vaccinia genome, it can accept large pieces of foreign DNA. Glycosylation in insect cells is not as complex as in mammalian cells and usually glycoproteins produced in insect cells are of slightly lower molecular mass than those produced in mammalian cells. The most common vector for expression of glycoproteins in insect cells is a baculovirus, Autographa californica nuclear polyhedrosis virus (AcNPV). The great advantage of this system is a very high level of expression of foreign genes.

Słowa kluczowe

EN

glycoprotein; animal virus; vaccinia; gene expression; baculovirus; gene coding

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 1999
• Tom: 46
• Numer: 2
• Opis fizyczny: p.325-339,fig.

Twórcy

autor

Bienkowska-Szewczyk K.

• University of Gdansk, Kladki 24, 80-822 Gdansk, Poland

autor

Szewczyk B.

Bibliografia

• Brown, C.S., van Lent, J.W., Vlak, J.M. & Spaan, W.J. (1991) Assembly of empty capsids by us­ing baculovirus recombinants expressing hu­man parvovirus B19 structural proteins. J. Vi­rol 65, 2702-2706.
• Chakrabarti, S., Brechling, K. & Moss, B. (1985) Vaccinia virus expression vector: Coexpres- sion of /J-galactosidase provides visual screen­ing of recombinant virus plaques. Mol Cell Biol 5, 3403-3409.
• Croizier, G., Croizier, L., Argaud, O. & Poudevi- gne, D. (1994) Extension of Autographa cali- fornica nuclear polyhedrosis virus host range by interspecific replacement of a short DNA sequence in the pl43 helicase gene. Proc. Natl Acad. Sci U.S.A. 91, 48-52.
• Dickson, J.A. & Friesen, P.D. (1991) Identifica­tion of upstream promoter elements mediat­ing early transcription from the 35,000- molecular weight protein gene of Autographa californica nuclear polyhedrosis virus. J. Vi­rol 65, 4006-4016.
• Doms, R.W., Blumenthal, R. & Moss, B. (1990) Fusion of intra- and extracellular forms of vac­cinia virus with the cell membrane. J. Virol 64, 4884-4892.
• Franke, C.A., Rice, C.M., Strauss, J.H. & Hruby, D.E. (1985) Neomycin resistance as a domi­nant selectable marker for selection and isola­tion of vaccinia virus recombinants. Mol Cell Biol 5, 1918-1924.
• Fraser, MJ. (1986) Ultrastructural observations of virion maturation in Autographa californica nuclear polyhedrosis virus infected Spodop- lera frugiperda cell cultures. J. Ultrastruct. Mol Struct Res. 95, 189-195.
• French, T.J., Marshall, J.J. & Roy, P. (1990) As­sembly of double-shelled, virus-like particles of bluetongue virus by the simultaneous ex­pression of four structural proteins. J. Virol 64, 5695-5700.
• Fuerst, T.R., Niles, E.G., Studier, F.W. & Moss, B. (1986) Eukaryotic transient>expression sys­tem based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polyme­rase. Proc. Nctl. Acad. ScL U.S.A. 83, 8122- 8126.
• Fuerst, T.R., Earl, P.L. & Moss. B. (1987) Use of a hybrid vaccinia virus T7 RNA polymerase sys­tem for expression of target genes. Mol Cell Biol 7, 2538-2544.
• Goebel, S.J., Johnson, G.P., Perkus, E., Davis, S.W., Winslow, J.P. & Paoletti, E. (1990) The complete DNA sequence of vaccinia virus. Vi­rology 179, 247-266.
• Hink, W.F. (1989) Recently established inverte­brate cell lines; in Invertebrate Cell System Ap­plications (Mitsuhashi, J., ed.) vol. 2, CRC Press, Boca Raton.
• Hollister, J.R., Shaper, J.H. & Jarvis, D.L. (1999) Stable expression of mammalian /?l,4-galacto- syltransferase extends the N-glycosylation pathway in insect cells. Glycobiology (in press).
• Jarvis, D.L. & Finn, E.E. (1996) Modifying the in­sect cell N-glycosylation pathway with imme­diate early baculovirus expression vectors. Nature Biotechnology 14, 1288-1292.
• Kitts, P.A., Ayres, M.D. & Possee, R.D. (1990) Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors. Nucleic Acids Res. 18, 5667-5672.
• Kitts, P.A. & Possee, R.D. (1993) A method for producing recombinant baculovirus expres­sion vectors at high frequency. Biotechniques 14, 810-817.
• Luckow, V.A., Lee, S.C., Barry, G.F. & Olins, P.O. (1993) Efficient generation of infectious re­combinant baculoviruses by site-specific transposon mediated insertion of foreign genes into a baculovirus genuine propagated in Escherichia coli. J. Virol. 67, 4566-4579.
• Mackett, M., Smith, G.L. & Moss, B. (1982) Vac­cinia virus: A selectable eukaryotic cloning and expression vector. Proc. Natl. Acad. ScL U.S.A 79, 7415-7419.
• Mackett, M., Smith, G.L. & Moss. B. (1984) Gen­eral method for production and selection of in­fectious vaccinia virus recombinants express­ing foreign genes. J. Virol 49, 857-864.
• Marz, L., Altmann, F., Staudacher, E. & Kubelko, V. (1995) Protein glycosylation in insects; in Glycoproteins (Montreuil, J., Vliegenthart, J.F. G. & Schachter, H., eds.) vol. 29, Elsevier, Am­sterdam.
• Miller, L.K., Lingg. A.J. & Bulla. L.A.J. (1983) Bacterial, viral and fungal insecticides. Sci­ence 219, 715-721.
• Mori, H., Nakazawa, H., Shirai, N., Shibata, N.. Sumida, M. & Matsubara, F. (1992) Foreign gene expression by a baculovirus vector with an expanded host range. J. Gen. Virol. 73, 1877-1880.
• Moss, B. (1991) Vaccinia virus: A tool for research and vaccine development. Science 252, 1662- 1667.
• Nissen, M.S. & Friesen, P.D. (1989) Molecular analysis of the transcriptional regulatory re­gion of an early baculovirus gene. J. Virol 63, 493-503.
• Panicali, D. & Paoletti, E. (1982) Construction of poxviruses as cloning vectors: Insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia vi­rus. Proc. Natl Acad. Sci. U.S.A. 79, 4927- 4931.
• Panicali, D., Grzelecki. A. & Huang, C. (1986) Vac­cinia virus vectors utilizing the /J-galactos- idase assay for rapid selection of recombinant viruses and measurement of gene expression. Gene 47, 193-199.
• Patel, G., Nasmyth, K. & Jones, N. (1992) A new method for the isolation of recombinant bacu­lovirus. Nucleic Acids Res. 20, 97-104.
• Peakman, T.C., Harris, R.A. & Gewert, D.R. (1992) Highly efficient generation of recombi­nant baculoviruses by enzymatically mediated site-specific in vitro recombination. Nucleic Acids Res. 20, 495-500.
• Roy, P. (1992) From genes to complex structures of bluetongue virus and their efficacy as vac­cines. Vet. Microbiol 33, 155-168.
• Rupprecht, C.E., Wiktor. T.J., Johnston, D.H., Hamir. A.N., Dietzschold, B., Wunner, W.H.,
• Glickman, L.T. & Koprowski, H. (1986) Oral immunization and protection of racoons (Pro- cyon lotor) with a vaccinia-rabies glycoprotein recombinant virus vaccine. Proc. Natl Acad. SciL U.S.A. 83, 7949-7950.
• Sabara, M., Parker, M., Aha, P., Cosco, C., Gib­bons, E., Parsons, S. & Babiuk, L.A. (1991) As­sembly of double-shelled rotaviruslike parti­cles by simultaneous expression of recombi­nant VP6 and VP7 proteins. J. Virol 65, 6994-6997.
• Schmelz, M., Sodeik, B., Ericsson, M., Wolffe, E., Shida, H., Hiller, G. & Griffiths, G. (1994) As­sembly of vaccinia virus: The second wrap­ping cisterna is derived from the trans Golgi network. J. Virol 68, 130-147.
• Smith, G.L. & Moss, B. (1983) Infectious poxvirus vectors have capacity for at least 25,000 base pairs of foreign DNA. Gene 25, 21-28.
• Sodeik, B., Doms, R.W., Ericsson, M., Hiller, G., Machamer, C.E., van't Hoff, W., van Meer, G., Moss, B. & Griffiths, G. (1993) Assembly of vaccinia virus: Role of the intermediate com­partment between the endoplasmic reticulum and the Gclgi stacks. J. Cell Biol 121, 521- 541.
• Summers, M.D. & Smith, G.E. (1987) A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agricultural Ex­perimental Station Bulletin 1555, 1-57.
• Van Der Wilk, F., Van Lent, J.W.M. & Vlak, J.M. (1987) Immunogold detection of polyhedrin, plO and virion antigens in Autographa califor- nica nuclear polyhedrosis virus-infected Spo- doptera frugiperda cells. J. Gen. Virol 68, 2615-2624.
• Van Oers, M.M., Flipsen, J.T.M., Reusken, C.B. E.M. & Vlak, J.M. (1994) Specificity of bacu­lovirus plO functions. Virology 200, 513-523.
• Vaughn, J.L., Goodwin, R.H., Tompkins, G.J. & McCawley, P. (1977) The establishment of two cell lines from the insect Spodoptera frugi­perda (Lepidoptera: Noctuidae). In Vitro 13, 213-217.
• Vaughn, J.L. & Weiss, S.A. (1991) Formulating media for the culture of insect cells. BioPharm 4, 16-19.
• Vialard, J., Lalumiere, M., Vernet, T., Briedis, D., Alkhatib, G., Henninh, D., Levin, D. & Rich­ardson, C. (1990) Synthesis of the membrane fusion and hemagglutinin proteins of measles virus, using a novel baculovirus vector con­taining the /3-galactosidase gene. J. Virol 64, 37-50.
• Wagner, R., Liedtke, S., Kretzschmar, E., Geyer, H., Geyer, R. & Klenk, H.D. (1996) Elongation of the N-glycans of fowl plague virus hemag­glutinin expressed in Spodoptera frugiperda (Sf9) cells by coexpression of human fi\,2-N- acetylglucosaminyltransferase I. Glycobiology 6, 165-175.
• Whitford, M., Stewart, S., Kuzio, J. & Faulkner, P. (1989) Identification and sequence analysis of a gene encoding gp67, an abundant enve­lope glycoprotein of the baculovirus Autogra­pha californica nuclear polyhedrosis virus. J. Virol 63, 1393-1399.
• Williams, G.V., Rohel, D.Z., Kuzio, J. & Faulkner, P. (1989) A cytopathological investigation of Autographa californica nuclear polyhedrosis virus plO gene function using insertion- deletion mutants. J. Gen. Virol 70,187-202.
• Wood, H.A. & Granados, R.R. (1991) Genetically engineered baculoviruses as agents for pest control. Annu. Rev. Microbiol 45, 69-87.
• Yuen, L. & Moss, B. (1987) Oligonucleotide se­quence signaling transcriptional termination of vaccinia virus early genes. Proc. Natl Acad. ScL U.S.A. 84, 6417-6421.
• Zhao, L.J., Irie, K., Trirawatanapong, T., Nakano, R., Nakashima, A., Morimatsu, M. & Padman- abhan, R. (1991) Synthesis of biologically ac­tive adenovirus preterminal protein in insect cells using a baculovirus vector. Gene 100, 147-154.
• Zuidema, D., Schouten, A., Usmany, M., Maule, A.J., Belsham, G.J., Roosien, J., Klinge-Rode, E.C., van Lent, J.W.M. & Vlak, J.M. (1990) Expression of cauliflower mosaic virus gene I in insect cells using a novel polyhedrin-based baculovirus expression vector. J. Gen. Virol. 71, 2201-2209.