Charakterystyka wybranych markerow patogennosci shigatoksycznych szczepow Escherichia coli wyosobnionych z surowej wolowiny przy uzyciu PCR

• Autorzy: Weiner M

Warianty tytułu

EN

Characterization of selected virulence markers of Shiga toxin-producing Escherichia coli originated from raw beef by the use of PCR

• Języki publikacji: PL

Abstrakty

EN

The aim of the study was the characterization of selected virulence markers of Shiga toxin-producing Escherichia coli originated from raw beef by the use of PCR. The identification of Stx variants (stx₂c, stx₂d, stx₂e and stx₂f) was done with the stx2 -positive E. coli strains, resulting in the detection of the stx2d gene in 13 STEC isolates out of which 3 strains also had the stx₂c gene. None of the isolates possessed the stx2f or stx2e genes. The intimin marker (γ variant) was observed in all E. coli O157:H7, whereas β variant in E. coli O26 isolates. The genotypic factors such as katP, toxB and efa1 were detected in all O157:H7 as well as in one O26 isolate. Fifteen STEC were iha-positive and five saa-positive. None of the saa-positive isolates belonged to the O157 or O26 groups. The simultaneous presence of the lpfAO₁₅₇/OI₋₁₄₁ and lpfAO₁₅₇/OI₋₁₅₄ genes was noted in 8 O157:H7 and in one O26 STEC. The lpfAO₁₅₇/OI₋₁₄₁ gene alone was present in 4 other STEC tested, including one E. coli O26. None of E. coli O157:H7 had the lpfAO113 marker that was observed in the all remaining STEC. In one isolate belonging to the E. coli O26 group, for the first time a simultaneous presence of the lpfAO₁₅₇/OI₋₁₄₁, lpfAO₁₅₇/OI₋₁₅₄ and lpfAO₁₁₃ was noted.

Słowa kluczowe

PL

zywnosc; mieso wolowe; zanieczyszczenia bakteryjne; Escherichia coli; szczepy shigatoksyczne; markery patogennosci; lancuchowa reakcja polimerazy

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2008
• Tom: 64
• Numer: 07
• Strony: 906-911
• Opis fizyczny: s.906-911,tab.,bibliogr.

Twórcy

autor

Weiner M

• Panstwowy Instytut Weterynaryjny - Panstwowy Instytut Badawczy, Al.Partyzantow 57, 24-100 Pulawy

Bibliografia

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