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2006 | 47 | 2 |
Tytuł artykułu

Screening for mutations affecting sexual reproduction after activation tagging in Arabidopsis thaliana

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In this work, a seed-set-based screening was performed on 70 lines of Arabidopsis thaliana after activation tagging mutagenesis to identify mutations in reproductive mechanisms. Five mutants showed significantly lower seed set than the wild type and confirmed the phenotype in the progeny. This phenotype was linked with the marker gene bar carried by T-DNA conferring glufosinate resistance. Genetic analysis revealed that the mutation inheritance was sporophytic in 3 mutants and gametophytic in 2 mutants. In addition, 2 mutants had an extra T-DNA copy. Thus activation tagging can be an effective strategy to identify new mutations affecting sporogenesis or gametogenesis.
Wydawca
-
Rocznik
Tom
47
Numer
2
Opis fizyczny
p.109-111,fig.,ref.
Twórcy
autor
  • DISSPA, Department of Soil, Plant and Environmental Sciences, University of Naples 'Federico II', Via Universita 133, 80055 Portici, Italy
autor
  • CNR-IGV, Institute of Plant Genetics, Research Division Portici, Via Universita, Portici, Italy
autor
  • CNR-IGV, Institute of Plant Genetics, Research Division Portici, Via Universita, Portici, Italy
autor
  • DISSPA, Department of Soil, Plant and Environmental Sciences, University of Naples 'Federico II', Via Universita 133, 80055 Portici, Italy
autor
  • Department Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana (USA)
autor
  • CNR-IGV, Institute of Plant Genetics, Research Division Portici, Via Universita, Portici, Italy
Bibliografia
  • Аkama K, Shiraishi Н, Ohta S, Nakamura K, Okada K, Shimura Y, 1992. Efficient transformation of Arabidopsis thaliana: comparison of the efficiencies with various organs, plant ecotypes and Agrobacterium strains. Plant Cell Rep 12:7-11.
  • Consiglio F, Carputo D, Monti L, Conicella C, 2004. Exploitation of genes affecting meiotic nonreduction and nuclear restitution: Arabidopsis as a model? Sex Plant Reprod 17: 97-105.
  • Drews GN, Lee D, Christensen GA, 1998. Genetic analysis of female gametophyte development and function. Plant Cell 10: 5-17.
  • Howden R, Park SK, Moore JM, Orme J, Grossniklaus U, Twell D, 1998. Selection of T-DNA tagged male and female gametophytic mutants by segregation distortion in Arabidopsis. Genetics 149: 621-631.
  • Ito T, Meyerowitz EM, 2000. Overexpression of a gene encoding a cytochrome P450, CYP78A9, induces large and seedless fruit in Arabidopsis. Plant Cell 12: 1541-1550.
  • Yadegari R, Drews GN, 2004. Female gametophyte development. Plant Cell 16: 133-141.
  • Weigel D, Ahn JH, Blazquez MA, Borevitz JO, Christensen SK, Fankhauser C, et al. 2000. Activation tagging in Arabidopsis. Plant Physiol 122: 1003-1013.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-a6bb9923-aae4-4b3a-8742-e567a08296dc
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