Screening for mutations affecting sexual reproduction after activation tagging in Arabidopsis thaliana

• Autorzy: Perrella G , Cremona G , Consiglio F , Errico A , Bressan R A , Conicella C
• Języki publikacji: EN

Abstrakty

EN

In this work, a seed-set-based screening was performed on 70 lines of Arabidopsis thaliana after activation tagging mutagenesis to identify mutations in reproductive mechanisms. Five mutants showed significantly lower seed set than the wild type and confirmed the phenotype in the progeny. This phenotype was linked with the marker gene bar carried by T-DNA conferring glufosinate resistance. Genetic analysis revealed that the mutation inheritance was sporophytic in 3 mutants and gametophytic in 2 mutants. In addition, 2 mutants had an extra T-DNA copy. Thus activation tagging can be an effective strategy to identify new mutations affecting sporogenesis or gametogenesis.

Słowa kluczowe

EN

genetics; activation tagging; gametophytic mutation; Arabidopsis thaliana; new mutation; sexual reproduction; sporophytic mutation; effective strategy; genetic analysis

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2006
• Tom: 47
• Numer: 2
• Opis fizyczny: p.109-111,fig.,ref.

Twórcy

autor

Perrella G

• DISSPA, Department of Soil, Plant and Environmental Sciences, University of Naples 'Federico II', Via Universita 133, 80055 Portici, Italy

autor

Cremona G

• CNR-IGV, Institute of Plant Genetics, Research Division Portici, Via Universita, Portici, Italy

autor

Consiglio F

• CNR-IGV, Institute of Plant Genetics, Research Division Portici, Via Universita, Portici, Italy

autor

Errico A

• DISSPA, Department of Soil, Plant and Environmental Sciences, University of Naples 'Federico II', Via Universita 133, 80055 Portici, Italy

autor

Bressan R A

• Department Horticulture and Landscape Architecture, Purdue University, West Lafayette, Indiana (USA)

autor

Conicella C

• CNR-IGV, Institute of Plant Genetics, Research Division Portici, Via Universita, Portici, Italy

Bibliografia

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