Zakazenia ran wywolane przez maczugowce reprezentujace gatunki bakterii uwazanych za oportunistyczne

• Autorzy: Olender A. , Letowska I.

Warianty tytułu

EN

Wound infections due to opportunistic Corynebacterium species

• Języki publikacji: PL

Abstrakty

PL

Przeprowadzono analizę 24 przypadków izolacji z ran w znamiennej ilości Gram-dodatnich maczugokształtnych pałeczek (C. amycolatum, C. striatum, C. grupa G, Brevibacterium sp., C. jeikeium, C. urealyticum, C. grupa F1). Scharakteryzowano oporność na antybiotyki na podstawie wartości MIC oznaczonych metodą Etest. U 83,3% szczepów występowała jednocześnie oporność na erytromycynę i klindamycynę (mechanizm MLSB), u 75% oporność na kotrimoksazol, u 71,7% oporność na chloramfenikol, u 16,7% na antybiotyki beta-lakatamowe. Wszystkie szczepy wykazywały wrażliwość na wankomycynę i teikoplaninę.

EN

Wound infections are often due to endogenous bacterial flora which penetrates into a site of injury. The establishment of the etiologie agent can be problematic, especially when opportunistic bacteria are present, suggesting contamination of clinical material. Among bacteria that can cause such diagnostic problems are opportunistic Corynebacterium spp. and coryneforms colonizing skin. The aim of the study was to analyze the 24 clinical samples collected from wounds of different location, with Gram positive rods isolated in numbers suggesting the cause of infection. Bacterial identification was performed by API Coryne and additional biochemical tests (API ZYM, API NE). It was detected that the commonest species isolated were: C. amycolatum (29,2%), C. striatum (16,7%), C. group G (16,7%) and Brevibacterium spp., C.jeikeium, C. urealyticum, C. group F1. The drug susceptibility testing was performed by E-test method. Among isolated strains, 83.3% were simultaneously resistant to erythromycin and clindamycin. In 75% cases resistance to co-trimoxazole was noted, in 71.7% resistance to chloramphenicol and in 16.7% resistance to beta-lactams were detected. In presented study the high percentage of strains resistant to macrolids and linkosamids (MLSB) was noted. All strains were susceptible to vancomycin and teicoplanin.

Słowa kluczowe

PL

flora bakteryjna; infekcja bakteryjna; zakazenia ran; maczugowce; opornosc na antybiotyki; wrazliwosc na leki; wankomycyna; teikoplanina

• Wydawca: -
• Czasopismo: Medycyna Doświadczalna i Mikrobiologia
• Rocznik: 2010
• Tom: 62
• Numer: 2
• Strony: 135-140
• Opis fizyczny: s.135-140,tab.,bibliogr.

Twórcy

autor

Olender A.

• Katedra i Zaklad Mikrobiologii Lekarskiej, Uniwersytet Medyczny w Lublinie, ul.W.Chodzki 1, 20-093 Lublin

autor

Letowska I.

Bibliografia

• 1. Blaise G, Arjen MD, Nikkels MD i inni. Corynebacterium - associated skin infections. Intern J Dermatol 2008; 47: 884-90
• 2. Boltin D, Katzir M, Boguslavsky V, Yalashvili I i inni. Corynebacterium striatum - a classic pathogen eluding diagnosis. Eur J Inter Med 2009; 20: 49-52
• 3. Bowler PG, Duerdet BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev 2001; 14: 244-69
• 4. Campanile F, Carretto E, Barbarini D i inni. Clonal multidrug-resistant Corynebacterium striatum strains Italy. Emerg Infect Dis 2009; 15: 75-8
• 5. Clinical Laboratory Standards Institute (CLSI). Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria; (ISBN 1-56238-607-7), 2009; M45-A, Vol. 26, No. 19
• 6. Coyle MB, Lipsky BA. Coryneform bacteria in infectious diseases: clinical and laboratory aspects. Clin Microbiol Rev 1990; 3: 227-46
• 7. Del Pozo JL, Tran NV, Petty PM i inni. Pilot study of association of bacteria on breast implants with capsular contracture. J Clin Microbiol 2009; 47: 1333-7
• 8. Frank DN, Wysocki A, Specht-Glick DD i inni. Microbial diversity in chronic open wounds. Wound Rep Reg 2009; 17: 163-72
• 9. Funke G, von Graevenitz A, Clarridge JEII. Clinical microbiology of coryneform bacteria. Clin Microbiol Rev 1997; 10: 125-59
• 10. Johnson AP, Mushtaq S, Warner M, Livermore DM. Activity of daptomycin against multi-resistant Gram-positive bacteria including enterococci and Staphylococcus aureus resistant to linezolid. Int J Antimicrob Agents 2004; 24: 315-9
• 11. Marti J, Anton E, Idoate A. Implantable cardioverter-defibrillator infection due to Corynebacterium xerosis. Int J Card 2008; 128: 1-2
• 12. Merhej V, Falsen E, Raoult D i inni. Corynebacterium timonense sp. nov. and Corynebacterium massilense sp. nov., isolated from human blood and human articular hip fluid. Int J Syst Evol Microbiol 2009; 59: 1953-9
• 13. Renaud FNR, Gregory A, Berreau C i inni. Identification of Turicella otitidis isolated from a patient with otorrhea associated with surgery: differentiation from Corynebacterium afermentans and Corynebacterium auris. J Clin Microbiol 1996; 34: 2625-7
• 14. Renaud FNR, Dutaur M, Daoud S i inni. Differention of Corynebacterium amycolatum, C. minutissimum, and C. striatum by carbon substrate assimilation tests. J Clin Microbiol 1998; 36: 3698-702
• 15. Superti SV, Martins DS, Caierao J i inni. Corynebacterium striatum infecting a malignant cutaneous lesion: the emergence of an opportunistic pathogen. Rev Inst Med Trop S Paulo 2009; 51: 115-6
• 16. Von Graevenitz A, Frommelt L, Punter-Streit V i inni. Diversity of coryneforms found in infections following prosthetic joint insertion and open fractures. Infection 1998; 26: 36-8
• 17. Wolcott RD, Gontcharova V, Sun У i inni. Evaluation of the bacterial diversity among and within individual venous leg ulcers using bacterial tag-encoded FLX and Titanium amplicon pyrosequencing and metagenomic approaches. BMC Microbiology 2009; 9: 226
• 18. Yague Guirao G, Mora Peris B, Martinez-Toldos MC i inni. Implication of ermX genes in macrolide- and telithromycin-resistance in Corynebacteriumjeikeium and Corynebacterium amycolatum. Rev Esp Quimioterap 2005; 3: 136-242