Amino acid substitutions of His296 alter the catalytic properties of Zymomonas mobilis 10232 levansucrase

• Autorzy: Li S.Y. , Chen M. , Li G. , Yan Y.L. , Yu H.Y. , Zhan Y.H. , Peng Z.X. , Wang J. , Lin M.
• Języki publikacji: EN

Abstrakty

EN

His296 of Zymomonas mobilis levansucrase (EC 2.4.1.10) is crucial for the catalysis of the transfructosylation reaction. The three-dimensional structures of levansucrases revealed the His296 is involved in the substrate recognition and binding. In this study, nine mutants were created by site-directed mutagenesis, in which His296 was substituted with amino acids of different polarity, charge and length. The substitutions of His296 with Arg or Trp retained partial hydrolysis and transfructosylation activities. The positively charged Lys substitution resulted in a 2.5-fold increase of sucrose hydrolysis. Substitutions with short (Cys or Ser), negatively charged (Glu) or polar (Tyr) amino acids virtually abolished both the activities. Analysis of transfructosylation products indicated that the mutants synthesized different oligosaccharides, suggesting that amino acid substitutions of His296 strongly affected both the enzyme activity and transfructosylation products.

Słowa kluczowe

EN

levan; enzyme activity; transfructosylation product; kinetic parameter; catalytic property; transfructosylation activity; amino acid; hydrolysis activity; oligosaccharide; sucrose hydrolysis; levansucrase; Zymomonas mobilis

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2008
• Tom: 55
• Numer: 1
• Opis fizyczny: p.201-206,fig.,ref.

Twórcy

autor

Li S.Y.

• Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. of China

autor

Chen M.

autor

Li G.

autor

Yan Y.L.

autor

Yu H.Y.

autor

Zhan Y.H.

autor

Peng Z.X.

autor

Wang J.

autor

Lin M.

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