Wplyw zwiekszonej zawartosci ozonu w powietrzu na wybrane wskazniki biochemiczne, hematologiczne i immunologiczne u szczurow w warunkach podawania w paszy zroznicowanych dawek witaminy E i C. II. Wplyw zwiekszonej zawar..

• Autorzy: Zielinski H , Jakubowski K.
• Języki publikacji: PL

Abstrakty

PL

Badania przeprowadzono na 180 szczurach szczepu Wistar/Hannower w trzech oddziel­nych doświadczeniach E, E+C i C. W każdym z doświadczeń zwierzęta dzielono na sześć grup w zależności od sposobu ekspozycji i dodatku witaminy E i С w paszy standardowej. Zwierzęta z grup kontrolnych oraz grup II żywiono paszą standardową dla gryzoni „Murigran", natomiast z grup III-VI w zależności od doświadczenia paszą wzbogaconą witaminą E, E+C lub C. Zwierzęta z grup II-VI eksponowano na 1.0±0.4 mg 03/ m3 powietrza przez okres 5 tygodni, 8 godz. dziennie. Po zakończeniu doświadczeń pobierano od szczurów płuca, wykonywano homogenat tkanki płucnej oraz otrzymywano z niego supernatant. W tkance płucnej metodą chromatografii gazowej oznaczono procentowy udział frakcji jedno i wielonienasyconych kwa­sów tłuszczowych, w homogenacie tkanki płucnej oznaczono poziom fluorescencji frakcji lipidowej i wodnej produktów peroksydacji lipidów, poziom dialdehydu małonowego i witaminy С metodą spektrofotometryczną, natomiast w supernatancie homogenatu określono zawartość białka całkowitego. Wyniki poddano analizie statystycznej. Stwierdzono niekorzystny wpływ ozonu na płuca zwierząt przejawiający się nasileniem procesów peroksydacji lipidów. Wykaza­no celowość żywienia szczurów paszą wzbogacaną witaminą E i С w warunkach narażenia ich na wielokrotnie powtarzane ekspozycje na powietrze o zwiększonej koncentracji ozonu.

EN

Three separate experiments (E, E+C and C) were carried out on 180 Wistar/Hannower rats. The rats were divided into six groups in each experiment depending on their exposure and the vitamin E and С supplement in the standard feed. The control animals and the rats from group II were fed the standard ŇMurigranÓ feed for rodents but animals in groups III— VI were fed feed enriched with vitamin E, E+C or C. The experimental animals from groups II-VI were exposed to 1.0ń0.4 mg 03/m3 air for 8 hours a day over 5 weeks. After the experiment, the rat lungs were separated, lung tissue was homogenised and supernatant was obtained. The gas chromatography technique was used to determine the share of mono- and polyunsaturated fatty acids in the lung tissue. The lipid and water percentage in the lipid peroxidation product fluorescence level, and the concentrations of malonyldialdehyde and vitamin С were determined with the use of the spectrophotometric technique. However, the total protein was determined in the homogenised lung tissue supernatant. The results were analysed statistically. A negative ozone influence on the rat lungs was observed resulting in the intensification of lipid peroxidation processes. Based on the experiments, it may be conclu­ded that feeding animals with the feed supplemented with vitamins E and С is beneficial for rats when exposed to air with elevated ozone concentrations.

Słowa kluczowe

PL

zwierzeta doswiadczalne; szczury; zywienie zwierzat; pasze; wskazniki biochemiczne; wskazniki hematologiczne; wskazniki immunologiczne; pluca; zawartosc witamin; witamina C; witamina E; zanieczyszczenia powietrza; ozon

EN

experimental animal; rat; animal feeding; feed; biochemical index; hematological index; immunological index; lung; vitamin content; vitamin C; vitamin E; air contaminant; ozone

• Wydawca: -
• Czasopismo: Acta Academiae Agriculturae ac Technicae Olstenensis. Veterinaria
• Rocznik: 1997
• Tom: 25
• Strony: 29-46
• Opis fizyczny: s.29-46,rys.,tab.,bibliogr.

Twórcy

autor

Zielinski H

• Instytut Rozrodu Zwierzat i Badan Zywnosci PAN, Olsztyn

autor

Jakubowski K.

Bibliografia

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