Mitochondria-associated satellite I RNA binds to hnRNP K protein

• Autorzy: Klimek-Tomczak K. , Mikula M. , Dzwonek A. , Paziewska A. , Wyrwicz L.S. , Hennig E.E. , Ostrowski J.
• Języki publikacji: EN

Abstrakty

EN

hnRNP K protein, which localizes to the nucleus, cytoplasm and mitochondria, is involved in the various cellular processes that compose gene expression. We used a SAGE-based assay to profile RNAs associated with hnRNP K protein in rat mitochondria. RNA was isolated from mitoplasts obtained from highly purified and RNase-treated mitochondria. Total RNA and RNA associated with hnRNP K protein were then used as input material for generating two SAGE libraries. Mitochondrion-derived tags isolated from the total mitoplast RNA library represented 86.3%, while those isolated from the library constructed from RNA associated with hnRNP K protein represented only 28.2% of selected tags. Thus, an unexpected number of nuclear-encoded RNAs were purified from mitochondria. Many of these transcripts were co-purified with hnRNP K protein, and high levels of nuclear-encoded RNAs co-immunoprecipitating with K protein corresponded to elevated hnRNP K protein levels of the organelle. The most abundant RNAs that were co-purified with hnRNP K protein represented transcripts originating from satellite I DNA. While satellite I RNA levels were higher in the nucleus and cytoplasm than in mitochondria, the most abundant binding of satellite I transcripts to hnRNP K protein was found in mitochondria. The role of satellite I RNA in mitochondria remains to be elucidated.

Słowa kluczowe

EN

green fluorescent protein; RNA; heterogenous nuclear ribonucleoprotein; mitochondrion; gene expression; ribonucleoprotein; protein; hnRNP K protein; polymerase chain reaction; cytoplasm; nucleus

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2006
• Tom: 53
• Numer: 1
• Opis fizyczny: p.169-178,fig.,ref.

Twórcy

autor

Klimek-Tomczak K.

• Medical Center for Postgraduate Education and the Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Warsaw, Poland

autor

Mikula M.

autor

Dzwonek A.

autor

Paziewska A.

autor

Wyrwicz L.S.

autor

Hennig E.E.

autor

Ostrowski J.

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